Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP

M. González del Pliego, E. Aguirre-Benítez, M. Del Carmen Sánchez-Soto, M. Larrieta, A. Velázquez-Carranza, R. Vidaltamayo, M. Meza-Mora, A. Zepeda-Rodríguez, J. Hernández-Falcón, M. Hiriart

Producción científicarevisión exhaustiva

10 Citas (Scopus)


Nerve growth factor (NGF) induces morphological and physiological changes in cultured pancreatic β-cells, including the extension of neurite-like processes. This latter effect is potentiated by dibutyryl cAMP (dbcAMP). β-cells cultured under these conditions maintain their immunoreactivity to insulin and gamma-amino-butyric acid (GABA). NGF, dbcAMP, and high glucose concentrations also increase the expression of the GABA-synthesizing enzyme glutamic acid decarboxylase-65 in cultured β-cells. The aim of this work was to study the effect of NGF alone or in combination with dbcAMP on pancreatic β-cell ultrastructural morphology, after 10 days in culture. We used light microscopy, scanning electron microscopy, and transmission electron microscopy to analyze the modifications in cell surface and neurite-like projections. Morphometric analysis showed that NGF and/or dbcAMP treatment substantially increased the insulin and GABA content in granules and rough endoplasmic reticulum. Given that pancreatic β-cells express NGF receptors and that NGF is synthesized and secreted by β-cells, these results further suggest that NGF could have trophic actions on pancreatic hormone synthesis and/or storage.
Idioma originalEnglish
Páginas (desde-hasta)365-378
Número de páginas14
PublicaciónCell and Tissue Research
EstadoPublished - 1 dic 2001
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Patología y medicina forense
  • Histología
  • Biología celular


Profundice en los temas de investigación de 'Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP'. En conjunto forman una huella única.

Citar esto