Type VII collagen regulates expression of OATP1B3, promotes front-to-rear polarity and increases structural organisation in 3D spheroid cultures of RDEB tumour keratinocytes

Jasbani H.S. Dayal, Clare L. Cole, Celine Pourreyron, Stephen A. Watt, Yok Zuan Lim, Julio C. Salas-Alanis, Dedee F. Murrell, John A. McGrath, Bruno Stieger, Colin Jahoda, Irene M. Leigh, Andrew P. South

Resultado de la investigaciónrevisión exhaustiva

17 Citas (Scopus)

Resumen

Type VII collagen is the main component of anchoring fibrils, structures that are integral to basement membrane homeostasis in skin. Mutations in the gene encoding type VII collagen COL7A1 cause recessive dystrophic epidermolysis bullosa (RDEB) an inherited skin blistering condition complicated by frequent aggressive cutaneous squamous cell carcinoma (cSCC). OATP1B3, which is encoded by the gene SLCO1B3, is a member of the OATP (organic anion transporting polypeptide) superfamily responsible for transporting a wide range of endogenous and xenobiotic compounds. OATP1B3 expression is limited to the liver in healthy tissues, but is frequently detected in multiple cancer types and is reported to be associated with differing clinical outcome. The mechanism and functional significance of tumour-specific expression of OATP1B3 has yet to be determined. Here, we identify SLCO1B3 expression in tumour keratinocytes isolated from RDEB and UV-induced cSCC and demonstrate that SLCO1B3 expression and promoter activity are modulated by type VII collagen. We show that reduction of SLCO1B3 expression upon expression of full-length type VII collagen in RDEB cSCC coincides with acquisition of front-to-rear polarity and increased organisation of 3D spheroid cultures. In addition, we show that type VII collagen positively regulates the abundance of markers implicated in cellular polarity, namely ELMO2, PAR3, E-cadherin, B-catenin, ITGA6 and Ln332.

Idioma originalEnglish
Páginas (desde-hasta)740-751
Número de páginas12
PublicaciónJournal of Cell Science
Volumen127
N.º4
DOI
EstadoPublished - 15 feb 2014
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Biología celular

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