Trichomonas vaginalis: Identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction

Javier Vargas-Villarreal, Benito D. Mata-Cárdenas, Francisco González-Salazar, Héctor G. Lozano-Garza, Elva I. Cortes-Gutierrez, Rebeca Palacios-Corona, Herminia G. Martínez-Rodríguez, Enrique Ramírez-Bon, Salvador Said-Fernández

Resultado de la investigación

9 Citas (Scopus)

Resumen

Trichomonad total extracts (TTE), or vesicular (P30) and soluble (S30) subcellular fractions from 3 pathogenic Trichomonas vaginalis strains (GT-3, GT-13, and GT-15), lysed both human and Sprague-Dawley rat erythrocytes in a time- and dose-dependent manner. The entire hemolytic activity of TTE was located in P30, showing 2 peaks of maximum activity, one at pH 6.0 and another at pH 8.0, in the presence of 1 mM Ca2+. Hemolytic activity on rat erythrocytes was greater at pH 6.0 (6.71 ± 0.33 hemolytic units [HU]/mg/hr to 11.60 ± 0.24 HU/mg/hr) than at pH 8.0 (3.81 ± 0.30 HU/mg/hr to 5.75 ± 0.65 HU/mg/hr), and it was greater than that on human red blood cells at pH 6.0 (2.67 ± 0.19 HU/mg/hr to 4.08 ± 0.15 HU/mg/hr) or pH 8.0 (2.24 ± 0.09 HU/mg/hr to 2.81 ± 0.06 HU/mg/hr). The alkaline and acidic hemolytic activity diminished (60-93% at pH 6.0 and 78-93% at pH 8.0) by the effect of 80 μM Rosenthal's inhibitor, which also inhibited 27-45% and 29-54% trichomonad alkaline and acidic phospholipase A activities, respectively. Vesicles, vacuoles, and hydrogenosomes were rich in P30. Trichomonas vaginalis has a hemolytic PLA, which could be involved in its cytopathogenic mechanism.
Idioma originalEnglish
Páginas (desde-hasta)105-112
Número de páginas8
PublicaciónJournal of Parasitology
DOI
EstadoPublished - 1 feb 2003
Publicado de forma externa

Huella dactilar

Trichomonas vaginalis
subcellular fractions
Subcellular Fractions
Phospholipases A
vesicle
inhibitor
blood
Trichomonadida
erythrocytes
Erythrocytes
phospholipases
effect
dose
rats
extracts
Vacuoles
vacuoles
Sprague Dawley Rats
calcium

Citar esto

Vargas-Villarreal, J., Mata-Cárdenas, B. D., González-Salazar, F., Lozano-Garza, H. G., Cortes-Gutierrez, E. I., Palacios-Corona, R., ... Said-Fernández, S. (2003). Trichomonas vaginalis: Identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction. Journal of Parasitology, 105-112. https://doi.org/10.1645/0022-3395(2003)089[0105:TVIOAP]2.0.CO;2
Vargas-Villarreal, Javier ; Mata-Cárdenas, Benito D. ; González-Salazar, Francisco ; Lozano-Garza, Héctor G. ; Cortes-Gutierrez, Elva I. ; Palacios-Corona, Rebeca ; Martínez-Rodríguez, Herminia G. ; Ramírez-Bon, Enrique ; Said-Fernández, Salvador. / Trichomonas vaginalis: Identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction. En: Journal of Parasitology. 2003 ; pp. 105-112.
@article{239801f16be142e498d00f13801be506,
title = "Trichomonas vaginalis: Identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction",
abstract = "Trichomonad total extracts (TTE), or vesicular (P30) and soluble (S30) subcellular fractions from 3 pathogenic Trichomonas vaginalis strains (GT-3, GT-13, and GT-15), lysed both human and Sprague-Dawley rat erythrocytes in a time- and dose-dependent manner. The entire hemolytic activity of TTE was located in P30, showing 2 peaks of maximum activity, one at pH 6.0 and another at pH 8.0, in the presence of 1 mM Ca2+. Hemolytic activity on rat erythrocytes was greater at pH 6.0 (6.71 ± 0.33 hemolytic units [HU]/mg/hr to 11.60 ± 0.24 HU/mg/hr) than at pH 8.0 (3.81 ± 0.30 HU/mg/hr to 5.75 ± 0.65 HU/mg/hr), and it was greater than that on human red blood cells at pH 6.0 (2.67 ± 0.19 HU/mg/hr to 4.08 ± 0.15 HU/mg/hr) or pH 8.0 (2.24 ± 0.09 HU/mg/hr to 2.81 ± 0.06 HU/mg/hr). The alkaline and acidic hemolytic activity diminished (60-93{\%} at pH 6.0 and 78-93{\%} at pH 8.0) by the effect of 80 μM Rosenthal's inhibitor, which also inhibited 27-45{\%} and 29-54{\%} trichomonad alkaline and acidic phospholipase A activities, respectively. Vesicles, vacuoles, and hydrogenosomes were rich in P30. Trichomonas vaginalis has a hemolytic PLA, which could be involved in its cytopathogenic mechanism.",
author = "Javier Vargas-Villarreal and Mata-C{\'a}rdenas, {Benito D.} and Francisco Gonz{\'a}lez-Salazar and Lozano-Garza, {H{\'e}ctor G.} and Cortes-Gutierrez, {Elva I.} and Rebeca Palacios-Corona and Mart{\'i}nez-Rodr{\'i}guez, {Herminia G.} and Enrique Ram{\'i}rez-Bon and Salvador Said-Fern{\'a}ndez",
year = "2003",
month = "2",
day = "1",
doi = "10.1645/0022-3395(2003)089[0105:TVIOAP]2.0.CO;2",
language = "English",
pages = "105--112",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",

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Vargas-Villarreal, J, Mata-Cárdenas, BD, González-Salazar, F, Lozano-Garza, HG, Cortes-Gutierrez, EI, Palacios-Corona, R, Martínez-Rodríguez, HG, Ramírez-Bon, E & Said-Fernández, S 2003, 'Trichomonas vaginalis: Identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction', Journal of Parasitology, pp. 105-112. https://doi.org/10.1645/0022-3395(2003)089[0105:TVIOAP]2.0.CO;2

Trichomonas vaginalis: Identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction. / Vargas-Villarreal, Javier; Mata-Cárdenas, Benito D.; González-Salazar, Francisco; Lozano-Garza, Héctor G.; Cortes-Gutierrez, Elva I.; Palacios-Corona, Rebeca; Martínez-Rodríguez, Herminia G.; Ramírez-Bon, Enrique; Said-Fernández, Salvador.

En: Journal of Parasitology, 01.02.2003, p. 105-112.

Resultado de la investigación

TY - JOUR

T1 - Trichomonas vaginalis: Identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction

AU - Vargas-Villarreal, Javier

AU - Mata-Cárdenas, Benito D.

AU - González-Salazar, Francisco

AU - Lozano-Garza, Héctor G.

AU - Cortes-Gutierrez, Elva I.

AU - Palacios-Corona, Rebeca

AU - Martínez-Rodríguez, Herminia G.

AU - Ramírez-Bon, Enrique

AU - Said-Fernández, Salvador

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Trichomonad total extracts (TTE), or vesicular (P30) and soluble (S30) subcellular fractions from 3 pathogenic Trichomonas vaginalis strains (GT-3, GT-13, and GT-15), lysed both human and Sprague-Dawley rat erythrocytes in a time- and dose-dependent manner. The entire hemolytic activity of TTE was located in P30, showing 2 peaks of maximum activity, one at pH 6.0 and another at pH 8.0, in the presence of 1 mM Ca2+. Hemolytic activity on rat erythrocytes was greater at pH 6.0 (6.71 ± 0.33 hemolytic units [HU]/mg/hr to 11.60 ± 0.24 HU/mg/hr) than at pH 8.0 (3.81 ± 0.30 HU/mg/hr to 5.75 ± 0.65 HU/mg/hr), and it was greater than that on human red blood cells at pH 6.0 (2.67 ± 0.19 HU/mg/hr to 4.08 ± 0.15 HU/mg/hr) or pH 8.0 (2.24 ± 0.09 HU/mg/hr to 2.81 ± 0.06 HU/mg/hr). The alkaline and acidic hemolytic activity diminished (60-93% at pH 6.0 and 78-93% at pH 8.0) by the effect of 80 μM Rosenthal's inhibitor, which also inhibited 27-45% and 29-54% trichomonad alkaline and acidic phospholipase A activities, respectively. Vesicles, vacuoles, and hydrogenosomes were rich in P30. Trichomonas vaginalis has a hemolytic PLA, which could be involved in its cytopathogenic mechanism.

AB - Trichomonad total extracts (TTE), or vesicular (P30) and soluble (S30) subcellular fractions from 3 pathogenic Trichomonas vaginalis strains (GT-3, GT-13, and GT-15), lysed both human and Sprague-Dawley rat erythrocytes in a time- and dose-dependent manner. The entire hemolytic activity of TTE was located in P30, showing 2 peaks of maximum activity, one at pH 6.0 and another at pH 8.0, in the presence of 1 mM Ca2+. Hemolytic activity on rat erythrocytes was greater at pH 6.0 (6.71 ± 0.33 hemolytic units [HU]/mg/hr to 11.60 ± 0.24 HU/mg/hr) than at pH 8.0 (3.81 ± 0.30 HU/mg/hr to 5.75 ± 0.65 HU/mg/hr), and it was greater than that on human red blood cells at pH 6.0 (2.67 ± 0.19 HU/mg/hr to 4.08 ± 0.15 HU/mg/hr) or pH 8.0 (2.24 ± 0.09 HU/mg/hr to 2.81 ± 0.06 HU/mg/hr). The alkaline and acidic hemolytic activity diminished (60-93% at pH 6.0 and 78-93% at pH 8.0) by the effect of 80 μM Rosenthal's inhibitor, which also inhibited 27-45% and 29-54% trichomonad alkaline and acidic phospholipase A activities, respectively. Vesicles, vacuoles, and hydrogenosomes were rich in P30. Trichomonas vaginalis has a hemolytic PLA, which could be involved in its cytopathogenic mechanism.

U2 - 10.1645/0022-3395(2003)089[0105:TVIOAP]2.0.CO;2

DO - 10.1645/0022-3395(2003)089[0105:TVIOAP]2.0.CO;2

M3 - Article

SP - 105

EP - 112

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

ER -