Trichomonas vaginalis: Identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction

Javier Vargas-Villarreal, Benito D. Mata-Cárdenas, Francisco González-Salazar, Héctor G. Lozano-Garza, Elva I. Cortes-Gutierrez, Rebeca Palacios-Corona, Herminia G. Martínez-Rodríguez, Enrique Ramírez-Bon, Salvador Said-Fernández

Resultado de la investigación

9 Citas (Scopus)

Resumen

Trichomonad total extracts (TTE), or vesicular (P30) and soluble (S30) subcellular fractions from 3 pathogenic Trichomonas vaginalis strains (GT-3, GT-13, and GT-15), lysed both human and Sprague-Dawley rat erythrocytes in a time- and dose-dependent manner. The entire hemolytic activity of TTE was located in P30, showing 2 peaks of maximum activity, one at pH 6.0 and another at pH 8.0, in the presence of 1 mM Ca2+. Hemolytic activity on rat erythrocytes was greater at pH 6.0 (6.71 ± 0.33 hemolytic units [HU]/mg/hr to 11.60 ± 0.24 HU/mg/hr) than at pH 8.0 (3.81 ± 0.30 HU/mg/hr to 5.75 ± 0.65 HU/mg/hr), and it was greater than that on human red blood cells at pH 6.0 (2.67 ± 0.19 HU/mg/hr to 4.08 ± 0.15 HU/mg/hr) or pH 8.0 (2.24 ± 0.09 HU/mg/hr to 2.81 ± 0.06 HU/mg/hr). The alkaline and acidic hemolytic activity diminished (60-93% at pH 6.0 and 78-93% at pH 8.0) by the effect of 80 μM Rosenthal's inhibitor, which also inhibited 27-45% and 29-54% trichomonad alkaline and acidic phospholipase A activities, respectively. Vesicles, vacuoles, and hydrogenosomes were rich in P30. Trichomonas vaginalis has a hemolytic PLA, which could be involved in its cytopathogenic mechanism.
Idioma originalEnglish
Páginas (desde-hasta)105-112
Número de páginas8
PublicaciónJournal of Parasitology
DOI
EstadoPublished - 1 feb 2003
Publicado de forma externa

    Huella digital

Citar esto

Vargas-Villarreal, J., Mata-Cárdenas, B. D., González-Salazar, F., Lozano-Garza, H. G., Cortes-Gutierrez, E. I., Palacios-Corona, R., Martínez-Rodríguez, H. G., Ramírez-Bon, E., & Said-Fernández, S. (2003). Trichomonas vaginalis: Identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction. Journal of Parasitology, 105-112. https://doi.org/10.1645/0022-3395(2003)089[0105:TVIOAP]2.0.CO;2