TY - JOUR
T1 - The inflammatory process modulates the expression and localization of WT1 in podocytes leading to kidney damage
AU - Arellano-Rodriguez, Mariela
AU - Zapata-Benavides, Pablo
AU - Arellano-Rodriguez, Norma Cesilia
AU - Izaguirre-Alvarez, Juan Manuel
AU - Franco-Molina, Moises Armides
AU - De Jesus Torres Del Muro, Felipe
AU - Mendoza-Gamboa, Edgar
AU - Soto-Dominguez, Adolfo
AU - Saavedra-Alonso, Santiago
AU - Rodriguez-Padilla, Cristina
N1 - Publisher Copyright:
© 2021 International Institute of Anticancer Research. All rights reserved.
PY - 2021/12
Y1 - 2021/12
N2 - Background/Aim: Wilms' tumor 1 (WT1) is involved in the development of the urogenital system and is expressed in podocytes throughout life. Inflammation of renal glomeruli causes renal damage-induced nephrotic syndrome and steroid-resistant nephrotic syndrome have mutations in the WT1 gene. The aim of this work was to determine if the inflammatory process modulates the expression and localization of WT1 in podocytes that cause kidney damage using lipopolysaccharide (LPS)-treated mice as a sepsis model. Materials and Methods: In investigation of renal damage, proteinuria and histology were analyzed. WT1 modulation was analyzed by indirect immunofluorescence, immunohistochemistry and western blot assays, and proinflammatory cytokines were analyzed by quantitative polymerase chain reaction assay. Results: WT1 expression decreased most at 24 and 36 h after the induction of inflammation and phosphorylated WT1 was mainly localized in the cytoplasm, reduced nephrin mRNA expression and increased mRNA expression of tumor necrosis factor a and interleukin 1β. Conclusion: These results indicate that the immune system plays an important role in the modulation of WT1, leading to kidney damage.
AB - Background/Aim: Wilms' tumor 1 (WT1) is involved in the development of the urogenital system and is expressed in podocytes throughout life. Inflammation of renal glomeruli causes renal damage-induced nephrotic syndrome and steroid-resistant nephrotic syndrome have mutations in the WT1 gene. The aim of this work was to determine if the inflammatory process modulates the expression and localization of WT1 in podocytes that cause kidney damage using lipopolysaccharide (LPS)-treated mice as a sepsis model. Materials and Methods: In investigation of renal damage, proteinuria and histology were analyzed. WT1 modulation was analyzed by indirect immunofluorescence, immunohistochemistry and western blot assays, and proinflammatory cytokines were analyzed by quantitative polymerase chain reaction assay. Results: WT1 expression decreased most at 24 and 36 h after the induction of inflammation and phosphorylated WT1 was mainly localized in the cytoplasm, reduced nephrin mRNA expression and increased mRNA expression of tumor necrosis factor a and interleukin 1β. Conclusion: These results indicate that the immune system plays an important role in the modulation of WT1, leading to kidney damage.
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U2 - 10.21873/invivo.12608
DO - 10.21873/invivo.12608
M3 - Article
C2 - 34697144
SN - 0258-851X
VL - 35
SP - 3137
EP - 3146
JO - In Vivo
JF - In Vivo
IS - 6
ER -