Monitoring the biochemical changes occurring to human keratinocytes exposed to solar radiation by Raman spectroscopy

Ulises Lopez-Gonzalez*, Alan Casey, Hugh J. Byrne

*Autor correspondiente de este trabajo

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4 Citas (Scopus)

Resumen

Solar radiation exposure is recognised to be a significant contributor to the development of skin cancer. Monitoring the simultaneous and consecutive mechanisms of interaction could provide a greater understanding of the process of photocarcinogenesis. This work presents an analysis of the biochemical and morphological changes occurring to immortalised human epithelial keratinocyte (HaCaT) cell cultures exposed to simulated solar radiation (SSR). Cell viability was monitored with the aid of the Alamar Blue assay, morphological examination was done with haematoxylin and eosin staining (H&E) and changes to the biochemical constituents (nucleic acids and proteins) as a result of the radiation insult were demonstrated through a combination of Raman microspectroscopy and multivariate analysis of spectral patterns. The spectral results suggest that SSR induces changes to the conformational structure of DNA as an immediate result of the radiation, whereas alteration in the protein signature is mostly seen as a later response.

Idioma originalEnglish
Número de artículoe202000337
Páginas (desde-hasta)e202000337
PublicaciónJournal of Biophotonics
Volumen14
N.º2
DOI
EstadoPublished - feb 2021
Publicado de forma externa

Nota bibliográfica

Publisher Copyright:
© 2020 Wiley-VCH GmbH

All Science Journal Classification (ASJC) codes

  • Química General
  • Ciencia de los Materiales General
  • Bioquímica, Genética y Biología Molecular General
  • Ingeniería General
  • Física y Astronomía General

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