Laccase-based catalytic microreactor for BPA biotransformation

Juan Eduardo Sosa-Hernández*, Elsa M. Gutierrez, Jhosseph S. Ochoa Sierra, Osvaldo Aquines Gutiérrez, Felipe Robledo-Padilla, Elda M. Melchor-Martínez, Hafiz M.N. Iqbal, Roberto Parra-Salvídar*

*Autor correspondiente de este trabajo

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Resumen

A laccase-based catalytic reactor was developed into a polydimethylsiloxane (PDMS) microfluidic device, allowing the degradation of different concentrations of the emergent pollutant, Bisphenol-A (BPA), at a rate similar to free enzyme. Among the immobilizing agents used, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was capable of immobilizing a more significant amount of the laccase enzyme in comparison to glutaraldehyde (GA), and the passive method (2989, 1537, and 1905 U/mL, respectively). The immobilized enzyme inside the microfluidic device could degrade 55 ppm of BPA at a reaction rate of 0.5309 U/mL*min with a contaminant initial concentration of 100 ppm at room temperature. In conclusion, the design of a microfluidic device and the immobilization of the laccase enzyme successfully allowed a high capacity of BPA degradation.

Idioma originalEnglish
Número de artículoe24483
Páginas (desde-hasta)e24483
PublicaciónHeliyon
Volumen10
N.º2
DOI
EstadoPublished - 30 ene 2024

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