TY - JOUR
T1 - Influence of whole-body washing of critically ill patients with chlorhexidine on Acinetobacter baumannii isolates
AU - Mendoza-Olazarán, Soraya
AU - Camacho-Ortiz, Adrian
AU - Martínez-Reséndez, Michel Fernando
AU - Llaca-Díaz, Jorge Martín
AU - Pérez-Rodríguez, Edelmiro
AU - Garza-González, Elvira
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/8
Y1 - 2014/8
N2 - Background Acinetobacter baumannii is 1 of the most important nosocomial pathogens and the causative agent of numerous types of infections, especially in intensive care units (ICUs). Our aim was to evaluate the effect of 2% chlorhexidine gluconate (CHG) whole-body washing of ICU patients on A baumannii in a tertiary care hospital. Methods During the 6-month intervention period, 327 patients were subjected to whole-body bath with 2% CHG-impregnated wipes. bla
IMP (active on imipenem), bla
VIM (Verona integron-encoded metallo-ß-lactamase), and bla
oxacillinase (OXA) of A baumannii were typed. Isolates were genotyped by pulsed-field gel electrophoresis. Minimum inhibitory concentrations (MIC) to CHG were determined by the agar dilution method and drug susceptibility determined using the broth microdilution method. Biofilm formation was determined by crystal violet staining. Results We analyzed 80 isolates during the baseline period and 69 isolates during the intervention period. There was a decrease in the MIC
50 and MIC
90 values for CHG for isolates (8 mg/L and 16 mg/L, respectively). All isolates typed positive for OXA
51-like and 86% typed positive for OXA
24-like pulsed-field gel electrophoresis identified 2 main clone types. During the intervention period the frequency of clone A decreased and that of clone B increased. Both clones were OXA
24-like positive. Conclusions The A baumannii isolates recovered from patients who received body washing with 2% CHG presented with a significant decrease in CHG MIC values associated with a change in clonality correlating with increased biofilm production.
AB - Background Acinetobacter baumannii is 1 of the most important nosocomial pathogens and the causative agent of numerous types of infections, especially in intensive care units (ICUs). Our aim was to evaluate the effect of 2% chlorhexidine gluconate (CHG) whole-body washing of ICU patients on A baumannii in a tertiary care hospital. Methods During the 6-month intervention period, 327 patients were subjected to whole-body bath with 2% CHG-impregnated wipes. bla
IMP (active on imipenem), bla
VIM (Verona integron-encoded metallo-ß-lactamase), and bla
oxacillinase (OXA) of A baumannii were typed. Isolates were genotyped by pulsed-field gel electrophoresis. Minimum inhibitory concentrations (MIC) to CHG were determined by the agar dilution method and drug susceptibility determined using the broth microdilution method. Biofilm formation was determined by crystal violet staining. Results We analyzed 80 isolates during the baseline period and 69 isolates during the intervention period. There was a decrease in the MIC
50 and MIC
90 values for CHG for isolates (8 mg/L and 16 mg/L, respectively). All isolates typed positive for OXA
51-like and 86% typed positive for OXA
24-like pulsed-field gel electrophoresis identified 2 main clone types. During the intervention period the frequency of clone A decreased and that of clone B increased. Both clones were OXA
24-like positive. Conclusions The A baumannii isolates recovered from patients who received body washing with 2% CHG presented with a significant decrease in CHG MIC values associated with a change in clonality correlating with increased biofilm production.
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U2 - 10.1016/j.ajic.2014.04.009
DO - 10.1016/j.ajic.2014.04.009
M3 - Article
C2 - 24913762
VL - 42
SP - 874
EP - 878
JO - American Journal of Infection Control
JF - American Journal of Infection Control
SN - 0196-6553
IS - 8
ER -