Induction of nestin early expression as a hallmark for mesenchymal stem cells expression of PDX-1 as a pre-disposing factor for their conversion into insulin producing cells

Marisela Martinez-Gamboa, Delia Elba Cruz-Vega, Jorge Moreno-Cuevas, Maria Teresa Gonzalez-Garza*

*Autor correspondiente de este trabajo

Resultado de la investigaciónrevisión exhaustiva

3 Citas (Scopus)

Resumen

Diabetes constitutes a worldwide epidemic that affects all ethnic groups. Cell therapy is one of the best alternatives of treatment, by providing an effective way to regenerate insulin-producing cells lost during the course of the disease, but many issues remain to be solved. Several groups have been working in the development of a protocol capable of differentiating Mesenchymal Stem Cells (MSCs) into physiologically sound Insulin Producing Cells (IPCs). In order to obtain a simple, fast and direct method, we propose in this manuscript the induction of MSCs to express NESTIN in a short time period (2 h), proceeded by incubation in a low glucose induced medium (24 h) and lastly by incubation in a high glucose medium. Samples from cell cultures incubated in high glucose medium from 12 to 168 h were obtained to detect the expression of INSULIN-1, INSULIN -2, PDX-1 and GLUT-2 genes. Induced cells were exposed to a glucose challenge, in order to assess the production of insulin. This method allowed us to obtain cells expressing PDX-1, which resembles a progenitor insulin-producing cell.

Idioma originalEnglish
Páginas (desde-hasta)76-82
Número de páginas7
PublicaciónInternational Journal of Stem Cells
Volumen10
N.º1
DOI
EstadoPublished - 1 may. 2017
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Biología del desarrollo
  • Biología celular

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