In-house validation of a rinse-membrane filtration method for processing fresh produce samples for downstream cultural detection of Salmonella, Escherichia coli O157:H7, and Listeria

Laura E. Tijerina-Rodríguez, Luisa Solís-Soto, Norma Heredia, Juan S. León, Lee Ann Jaykus, Santos García

Resultado de la investigaciónrevisión exhaustiva

Resumen

More efficient sampling and detection methods of pathogens on fresh produce are needed. The purpose of this study was to compare a novel rinse-membrane filtration method (RMFM) to a more traditional sponge rubbing or stomaching method in processing jalapeño peppers and cantaloupe samples for detection of Escherichia coli, Salmonella enterica, and Listeria monocytogenes. For jalapeño peppers inoculated with 106, 104, and 102 CFU of each pathogen and cantaloupes inoculated at 106 and 104 CFU, all pathogens were detected in all (100%) samples by RMFM at a 10-mL filtration volume, as well as by the stomacher and sponge rubbing methods. However, for cantaloupe inoculated at 102 CFU, detection differed by pathogen: S. enterica (20% RMFM, 60% stomacher, and 20% sponge), L. monocytogenes (40% RMFM, 60% stomacher, and 20% sponge), and E. coli O157:H7 (100% RMFM, 75% stomacher, and 75% sponge). When RMFM was compared with the other methods, in accordance with guidelines in the International Organization for Standardization 16140:2003 protocol, it produced values >95% in relative accuracy, relative specificity, and relative sensitivity. Overall, the RMFM performed similar to or better than the homogenization and sponge surface rubbing methods and is a good alternative for processing large numbers of produce samples for bacterial pathogen detection.

Idioma originalEnglish
Páginas (desde-hasta)1592-1597
Número de páginas6
PublicaciónJournal of Food Protection
Volumen83
N.º9
DOI
EstadoPublished - sept. 2020
Publicado de forma externa

Nota bibliográfica

Publisher Copyright:
Copyright © International Association for Food Protection

All Science Journal Classification (ASJC) codes

  • Alimentación
  • Microbiología

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Profundice en los temas de investigación de 'In-house validation of a rinse-membrane filtration method for processing fresh produce samples for downstream cultural detection of Salmonella, Escherichia coli O157:H7, and Listeria'. En conjunto forman una huella única.

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