TY - JOUR
T1 - In-house validation of a rinse-membrane filtration method for processing fresh produce samples for downstream cultural detection of Salmonella, Escherichia coli O157:H7, and Listeria
AU - Tijerina-Rodríguez, Laura E.
AU - Solís-Soto, Luisa
AU - Heredia, Norma
AU - León, Juan S.
AU - Jaykus, Lee Ann
AU - García, Santos
N1 - Funding Information:
This work was supported by the Universidad Autónoma de Nuevo León. Laura E. Tijerina-Rodríguez acknowledges a postgraduate fellowship from Consejo Nacional de Ciencia y Tecnología. Juan. S. Leon was partially supported by National Institute of Food and Agriculture grants (2015-67017-23080 and 2018-07410).
Publisher Copyright:
Copyright © International Association for Food Protection
PY - 2020/9
Y1 - 2020/9
N2 - More efficient sampling and detection methods of pathogens on fresh produce are needed. The purpose of this study was to compare a novel rinse-membrane filtration method (RMFM) to a more traditional sponge rubbing or stomaching method in processing jalapeño peppers and cantaloupe samples for detection of Escherichia coli, Salmonella enterica, and Listeria monocytogenes. For jalapeño peppers inoculated with 106, 104, and 102 CFU of each pathogen and cantaloupes inoculated at 106 and 104 CFU, all pathogens were detected in all (100%) samples by RMFM at a 10-mL filtration volume, as well as by the stomacher and sponge rubbing methods. However, for cantaloupe inoculated at 102 CFU, detection differed by pathogen: S. enterica (20% RMFM, 60% stomacher, and 20% sponge), L. monocytogenes (40% RMFM, 60% stomacher, and 20% sponge), and E. coli O157:H7 (100% RMFM, 75% stomacher, and 75% sponge). When RMFM was compared with the other methods, in accordance with guidelines in the International Organization for Standardization 16140:2003 protocol, it produced values >95% in relative accuracy, relative specificity, and relative sensitivity. Overall, the RMFM performed similar to or better than the homogenization and sponge surface rubbing methods and is a good alternative for processing large numbers of produce samples for bacterial pathogen detection.
AB - More efficient sampling and detection methods of pathogens on fresh produce are needed. The purpose of this study was to compare a novel rinse-membrane filtration method (RMFM) to a more traditional sponge rubbing or stomaching method in processing jalapeño peppers and cantaloupe samples for detection of Escherichia coli, Salmonella enterica, and Listeria monocytogenes. For jalapeño peppers inoculated with 106, 104, and 102 CFU of each pathogen and cantaloupes inoculated at 106 and 104 CFU, all pathogens were detected in all (100%) samples by RMFM at a 10-mL filtration volume, as well as by the stomacher and sponge rubbing methods. However, for cantaloupe inoculated at 102 CFU, detection differed by pathogen: S. enterica (20% RMFM, 60% stomacher, and 20% sponge), L. monocytogenes (40% RMFM, 60% stomacher, and 20% sponge), and E. coli O157:H7 (100% RMFM, 75% stomacher, and 75% sponge). When RMFM was compared with the other methods, in accordance with guidelines in the International Organization for Standardization 16140:2003 protocol, it produced values >95% in relative accuracy, relative specificity, and relative sensitivity. Overall, the RMFM performed similar to or better than the homogenization and sponge surface rubbing methods and is a good alternative for processing large numbers of produce samples for bacterial pathogen detection.
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U2 - 10.4315/JFP-19-581
DO - 10.4315/JFP-19-581
M3 - Article
C2 - 32421788
AN - SCOPUS:85090176983
SN - 0362-028X
VL - 83
SP - 1592
EP - 1597
JO - Journal of Food Protection
JF - Journal of Food Protection
IS - 9
ER -