Galectin labeling of cells from paraffinized tissues may serve as a diagnostic tool in the detection and classification of thyroid carcinomas

Rebeca Palacios-Corona, Francisco González-Salazar, Ricardo M. Cerda-Flores, Javier Vargas-Villarreal, Eduardo González-Murillo, Hugo Gutiérrez-Hermosillo, Hugo Gómez-Rueda, Lorena Tamez-Peña, Gerardo Rivera-Silva, Héctor Eloy Tamez-Pérez

Resultado de la investigación

Resumen

This study seeks to determine whether the relative levels of attachment to galectins 1 and 3 of cells from thyroid tissues embedded in paraffin blocks can differentiate thyroid tumors from normal tissues. A total of 48 thyroid paraffin sample blocks from 4 groups of patients were analyzed: 12 samples served as controls, 12 samples were from patients with thyroid adenoma, 12 samples were from patients with thyroid follicular carcinoma, and 12 samples were from patients with thyroid papillary carcinoma. The relative attachment of cells to galectins 1 and 3 antigens was determined using the InnoCyte™ ECM Cell Adhesion kit at different cell sample concentrations. All of the samples from thyroid tissue preparations showed attachment to galectins 1 and 3. The samples from tissues with a diagnosis of adenoma, follicular and papillary carcinoma showed an increased adherence to galectins 1 and 3 relative to the controls. Significant differences were found between the means of the adherent cells from the adenomas compared with the follicular and papillary carcinoma samples. When the outcomes from the galectins 1 and 3 cell surface binding were compared, no statistical differences were found. The cells from adenoma and carcinoma samples show more adhesion to galectins 1 and 3 than cells from the control samples. The samples prepared from follicular and papillary carcinomas show more cells adherent to galectins 1 and 3 than those from the adenomas. © 2012 Springer Science+Business Media, LLC.
Idioma originalEnglish
Páginas (desde-hasta)161-167
Número de páginas7
PublicaciónEndocrine Pathology
DOI
EstadoPublished - 1 sep 2012
Publicado de forma externa

    Huella digital

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Citar esto

Palacios-Corona, R., González-Salazar, F., Cerda-Flores, R. M., Vargas-Villarreal, J., González-Murillo, E., Gutiérrez-Hermosillo, H., Gómez-Rueda, H., Tamez-Peña, L., Rivera-Silva, G., & Tamez-Pérez, H. E. (2012). Galectin labeling of cells from paraffinized tissues may serve as a diagnostic tool in the detection and classification of thyroid carcinomas. Endocrine Pathology, 161-167. https://doi.org/10.1007/s12022-012-9215-x