Effects of thalidomide and pentoxyphylline over local and remote organ injury after intestinal ischemia/reperfusion

C R Cámara-Lemarroy, F J Guzmán-de la Garza, G Alarcón-Galván, P Cordero-Pérez, L E Muñoz-Espinosa, N E Fernández-Garza

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12 Citas (Scopus)

Resumen

Objective: We investigated the effects of thalidomide alone or in combination with pentoxyphylline upon intestinal ischemia/reperfusion (I/R) injury in the rat. Materials and Methods: Twenty male Wistar rats were randomized into 5 groups: sham-operated (SHAM), control (CTL), thalidomide (400 mg/kg) treatment (THAL), pentoxyphylline (50 mg/kg) treatment and a combination group (THAL + POX). I/R was induced by clamping the superior mesenteric artery for 45 minutes, followed by 120 minutes of reperfusion. We measured serum concentrations of aspartate-aminotransferase (AST), lactate dehydrogenase (LDH), tumor necrosis factor (TNF)-α as well as lipid peroxidation and antioxidant status. Intestinal samples were morphologically analyzed, and dry to wet (W/D) ratios calculated in intestinal, lung and liver samples, as a measurement of tissue edema. Results: Serum concentrations of AST, LDH, and TNF-α were increased after I/R in the CTL compared with the SHAM group (P < .05). Lipid peroxidation was also increased, and antioxidant capacity in serum, decreased (P < .05). The W/D ratio was elevated in all tissue samples as well (P < .05). Both thalidomide and pentoxyphylline effectively reduced AST, LDH, TNF-α, and lipid peroxidation levels, as well as attenuated tissue edema and intestinal injury induced by I/R (P < .05). Combination treatment showed only modest additive effects on lung W/D ratio and TNF-α levels. Conclusion: Both drugs protected the intestine, lungs, and liver against intestinal I/R injury, probably by inhibition of TNF-α and lipid peroxidation. However, combination treatment showed small, additive effects.

Idioma originalEnglish
Páginas (desde-hasta)1624-1626
Número de páginas3
PublicaciónTransplantation Proceedings
Volumen42
N.º5
DOI
EstadoPublished - jun 2010
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Cirugía
  • Transplantes

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