TY - JOUR
T1 - Comparison of gene expression profiles between pansensitive and multidrug-resistant strains of mycobacterium tuberculosis
AU - Peñuelas-Urquides, K.
AU - González-Escalante, L.
AU - Villarreal-Treviño, L.
AU - Silva-Ramírez, B.
AU - Gutiérrez-Fuentes, D. J.
AU - Mojica-Espinosa, R.
AU - Rangel-Escareño, C.
AU - Uribe-Figueroa, L.
AU - Molina-Salinas, G. M.
AU - Dávila-Velderrain, J.
AU - Castorena-Torres, F.
AU - Bermúdez De León, M.
AU - Said-Fernández, S.
N1 - Funding Information:
This study was supported by the National Council for Science and Technology (CONACyT), Mexico, Grant No. 99792.
PY - 2013/9/1
Y1 - 2013/9/1
N2 - Mycobacterium tuberculosis has developed resistance to anti-tuberculosis first-line drugs. Multidrug-resistant strains complicate the control of tuberculosis and have converted it into a worldwide public health problem. Mutational studies of target genes have tried to envisage the resistance in clinical isolates; however, detection of these mutations in some cases is not sufficient to identify drug resistance, suggesting that other mechanisms are involved. Therefore, the identification of new markers of susceptibility or resistance to first-line drugs could contribute (1) to specifically diagnose the type of M. tuberculosis strain and prescribe an appropriate therapy, and (2) to elucidate the mechanisms of resistance in multidrug-resistant strains. In order to identify specific genes related to resistance in M. tuberculosis, we compared the gene expression profiles between the pansensitive H37Rv strain and a clinical CIBIN:UMF:15:99 multidrug-resistant isolate using microarray analysis. Quantitative real-time PCR confirmed that in the clinical multidrug-resistant isolate, the esxG, esxH, rpsA, esxI, and rpmI genes were upregulated, while the lipF, groES, and narG genes were downregulated. The modified genes could be involved in the mechanisms of resistance to first-line drugs in M. tuberculosis and could contribute to increased efficiency in molecular diagnosis approaches of infections with drug-resistant strains.
AB - Mycobacterium tuberculosis has developed resistance to anti-tuberculosis first-line drugs. Multidrug-resistant strains complicate the control of tuberculosis and have converted it into a worldwide public health problem. Mutational studies of target genes have tried to envisage the resistance in clinical isolates; however, detection of these mutations in some cases is not sufficient to identify drug resistance, suggesting that other mechanisms are involved. Therefore, the identification of new markers of susceptibility or resistance to first-line drugs could contribute (1) to specifically diagnose the type of M. tuberculosis strain and prescribe an appropriate therapy, and (2) to elucidate the mechanisms of resistance in multidrug-resistant strains. In order to identify specific genes related to resistance in M. tuberculosis, we compared the gene expression profiles between the pansensitive H37Rv strain and a clinical CIBIN:UMF:15:99 multidrug-resistant isolate using microarray analysis. Quantitative real-time PCR confirmed that in the clinical multidrug-resistant isolate, the esxG, esxH, rpsA, esxI, and rpmI genes were upregulated, while the lipF, groES, and narG genes were downregulated. The modified genes could be involved in the mechanisms of resistance to first-line drugs in M. tuberculosis and could contribute to increased efficiency in molecular diagnosis approaches of infections with drug-resistant strains.
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UR - https://www.mendeley.com/catalogue/33df18f6-0528-373e-a8c7-731995f31b2c/
U2 - 10.1007/s00284-013-0376-8
DO - 10.1007/s00284-013-0376-8
M3 - Article
SN - 0343-8651
VL - 67
SP - 362
EP - 371
JO - Current Microbiology
JF - Current Microbiology
IS - 3
ER -
