Characterization of a cDNA clone encoding molluscan insulin‐related peptide II of Lymnaea stagnalis

August B. SMIT, Wijnand P.M. GERAERTS, Irene MEESTER, Harm van HEERIKHUIZEN, Joos JOOSSE

Resultado de la investigación

41 Citas (Scopus)

Resumen

A cDNA clone encoding molluscan insulin‐related peptide (MIP) II was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnaea stagnalis, using a heterologous screening with a previously identified MIP cDNA (renamed MIP‐I cDNA). The MIP‐II cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and A and B chains; however, in this case connected by two distinct C peptides, Cα and Cβ, instead of a single C peptide, a phenomenon which represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIP II and I differ remarkably in primary structure; in contrast, the Cα peptide domains are fully identical. MIP II has only limited sequence similarity with insulins and related peptides. Both MIP II and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I and II genes are expressed in a single type of neuron: the growth‐controlling neuroendocrine light green cells of the Lymnaea CNS. Copyright © 1991, Wiley Blackwell. All rights reserved
Idioma originalEnglish
Páginas (desde-hasta)699-703
Número de páginas5
PublicaciónEuropean Journal of Biochemistry
DOI
EstadoPublished - 1 ene 1991
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Biochemistry

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