Axenic cultivation and comparative phospholipase A<inf>2</inf> activity of Giardia duodenalis in a serum-free medium

Benito David Mata-Cárdenas, Magda Elizabeth Hernández-García, Francisco González-Salazar, Jesús Norberto Garza González, Rebeca Palacios-Corona, Elva I. Cortés-Gutiérrez, Javier Vargas-Villarreal

Resultado de la investigaciónrevisión exhaustiva

3 Citas (Scopus)

Resumen

Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle's amino acid solution, Diamond's vitamin-tween 80 mixtures and LCR (a lipid-cholesterol - rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A 2 (PLA A 2) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95% compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A 2 activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum.

Idioma originalEnglish
Páginas (desde-hasta)211-215
Número de páginas5
PublicaciónActa Parasitologica
Volumen57
N.º3
DOI
EstadoPublished - 1 sep 2012
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Parasitología

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