Antibody against Small Aggregated Peptide Specifically Recognizes Toxic Aβ-42 Oligomers in Alzheimer's Disease

R.U. Bodani, U. Sengupta, D.L. Castillo-Carranza, M.J. Guerrero-Muñoz, J.E. Gerson, J. Rudra, R. Kayed

Resultado de la investigaciónrevisión exhaustiva

15 Citas (Scopus)

Resumen

Amyloid-beta (Aβ) oligomers have emerged as the most toxic species in Alzheimer's disease (AD) and other amyloid pathologies. Also, Aβ-42 peptide is more aggregation-prone compared to other Aβ isoforms. Thus, we synthesized a small peptide of repeated sequence containing the last three amino acids, Val-40, Ile-41, and Ala-42 of Aβ-42 that was subsequently aggregated and used to generate a novel antibody, VIA. In this study, we examined human AD and Tg2576 mouse brain samples using VIA in combination with other amyloid-specific antibodies and confirmed the specificity of VIA to oligomeric Aβ-42. Moreover, we found that VIA does not recognize classic amyloid plaques composed of fibrillar Aβ or Aβ-40 ex vivo. Since VIA recognizes a distinct epitope specific to Aβ-42 oligomers, it may have broad use for examining the accumulation of these oligomers in AD and other neurodegenerative diseases. VIA may also be used in immunotherapy studies to prevent neurodegenerative effects associated with Aβ-42 oligomers.

Idioma originalEnglish
Páginas (desde-hasta)1981-1989
Número de páginas9
PublicaciónACS Chemical Neuroscience
Volumen6
N.º12
DOI
EstadoPublished - 16 dic 2015
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Bioquímica
  • Fisiología
  • Neurociencia cognitiva
  • Biología celular

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