Advantages of adipose tissue stem cells over CD34+ mobilization to decrease hepatic fibrosis in Wistar rats

Marcela M. De Luna-Saldivar, Iván A. Marino-Martinez, Moisés A. Franco-Molina, Lydia G. Rivera-Morales, Gabriela Alarcón-Galván, Paula Cordero-Pérez, Augusto Rojas-Martínez, Cristina Rodríguez-Padilla, Linda E. Muñoz-Espinosa

Resultado de la investigación

4 Citas (Scopus)

Resumen

Introduction and Objectives: Chronic liver inflammation may lead to hepatic cirrhosis, limiting its regenerative capacity. The clinical standard of care is transplantation, although stem cell therapy may be an alternative option. The study aim was to induce endogenous hematopoietic stem cells (HSCs) with granulocyte colony stimulating factor (G-CSF) and/or intravenous administration of adipose tissue-derived mesenchymal stem cells (MSCs) to decrease hepatic fibrosis in an experimental model. Material and methods: A liver fibrosis model was developed with female Wistar rats via multiple intraperitoneal doses of carbon tetrachloride. Three rats were selected to confirm cirrhosis, and the rest were set into experimental groups to evaluate single and combined therapies of G-CSF-stimulated HSC mobilization and intravenous MSC administration. Results: Treatment with MSCs and G-CSF significantly improved alanine amino transferase levels, while treatment with G-CSF, MSCs, and G-CSF + MSCs decreased aspartate amino transferase levels. Hepatocyte growth factor (HGF) and interleukin 10 levels increased with MSC treatment. Transforming growth factor β levels were lower with MSC treatment. Interleukin 1β and tumor necrosis factor alpha levels decreased in all treated groups. Histopathology showed that MSCs and G-CSF reduced liver fibrosis from F4 to F2. Conclusions: MSC treatment improves liver function, decreases hepatic fibrosis, and plays an anti-inflammatory role; it promotes HGF levels and increased proliferating cell nuclear antigen when followed by MSC treatment mobilization using G-CSF. When these therapies were combined, however, fibrosis improvement was less evident. © 2019 Fundación Clínica Médica Sur, A.C.
Idioma originalEnglish
Páginas (desde-hasta)620-626
Número de páginas7
PublicaciónAnnals of Hepatology
Volumen18
N.º4
DOI
EstadoPublished - 1 jul 2019

All Science Journal Classification (ASJC) codes

  • Hepatology

Huella Profundice en los temas de investigación de 'Advantages of adipose tissue stem cells over CD34<sup>+</sup> mobilization to decrease hepatic fibrosis in Wistar rats'. En conjunto forman una huella única.

  • Citar esto

    De Luna-Saldivar, M. M., Marino-Martinez, I. A., Franco-Molina, M. A., Rivera-Morales, L. G., Alarcón-Galván, G., Cordero-Pérez, P., Rojas-Martínez, A., Rodríguez-Padilla, C., & Muñoz-Espinosa, L. E. (2019). Advantages of adipose tissue stem cells over CD34+ mobilization to decrease hepatic fibrosis in Wistar rats. Annals of Hepatology, 18(4), 620-626. https://doi.org/10.1016/j.aohep.2018.12.005