β-naphthoflavone represses dystrophin Dp71 expression in Hepa-1 cells

Mario Bermúdez de León, Pablo Gómez, Guillermo Elizondo, Rocío Zatarain-Palacios, Francisco García-Sierra, Bulmaro Cisneros

Resultado de la investigación

5 Citas (Scopus)

Resumen

Dystrophin Dp71 is expressed in hepatic tissue; however, its function in this tissue remains unknown. The Dp71 promoter sequence contains conserved CACGC motifs, which constitute the invariant core sequence of xenobiotic-regulatory elements. These elements function as target sites for the aryl hydrocarbon receptor/aryl hydrocarbon nuclear translocator (Ahr/ARNT) in genes regulated by this transcription factor. Thus, Dp71 expression in hepatic cells would be regulated by Ahr signaling. In this study, the effect of the xenobiotics β-Naphthoflavone (βNF), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and Benzo[a]Pyrene (BaP) on Dp71 expression was analyzed in Hepa-1 cells. It was demonstrated that βNF, but not BaP or TCDD, represses Dp71 expression at both transcriptional and translational levels. To test directly the involvement of the Ahr signaling in the negative regulation of Dp71, we analyzed the effect of βNF on Dp71 expression in the liver of wild type (Ahr+/+) and AHR-null (Ahr-/-) mice. The Dp71 mRNA repression, caused by the βNF treatment, was also found in the liver tissue of wild type mice; however, such negative effect was reversed in the liver of AHR-null mice, which supports the participation of the Ahr signaling in Dp71 downregulation. Modulation of Dp71 expression by βNF may represent a novel mechanism of Ahr action. © 2006 Elsevier B.V. All rights reserved.
Idioma originalEnglish
Páginas (desde-hasta)152-158
Número de páginas7
PublicaciónBiochimica et Biophysica Acta - Gene Structure and Expression
DOI
EstadoPublished - 1 mar 2006
Publicado de forma externa

Huella dactilar

Dystrophin
Liver
Xenobiotics
Aryl Hydrocarbon Receptor Nuclear Translocator
Aryl Hydrocarbon Receptors
Conserved Sequence
Benzo(a)pyrene
Hepatocytes
Transcription Factors
Down-Regulation
Messenger RNA
Genes
Polychlorinated Dibenzodioxins

Citar esto

Bermúdez de León, Mario ; Gómez, Pablo ; Elizondo, Guillermo ; Zatarain-Palacios, Rocío ; García-Sierra, Francisco ; Cisneros, Bulmaro. / β-naphthoflavone represses dystrophin Dp71 expression in Hepa-1 cells. En: Biochimica et Biophysica Acta - Gene Structure and Expression. 2006 ; pp. 152-158.
@article{378219f8129a43a1a4453ffb0f350742,
title = "β-naphthoflavone represses dystrophin Dp71 expression in Hepa-1 cells",
abstract = "Dystrophin Dp71 is expressed in hepatic tissue; however, its function in this tissue remains unknown. The Dp71 promoter sequence contains conserved CACGC motifs, which constitute the invariant core sequence of xenobiotic-regulatory elements. These elements function as target sites for the aryl hydrocarbon receptor/aryl hydrocarbon nuclear translocator (Ahr/ARNT) in genes regulated by this transcription factor. Thus, Dp71 expression in hepatic cells would be regulated by Ahr signaling. In this study, the effect of the xenobiotics β-Naphthoflavone (βNF), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and Benzo[a]Pyrene (BaP) on Dp71 expression was analyzed in Hepa-1 cells. It was demonstrated that βNF, but not BaP or TCDD, represses Dp71 expression at both transcriptional and translational levels. To test directly the involvement of the Ahr signaling in the negative regulation of Dp71, we analyzed the effect of βNF on Dp71 expression in the liver of wild type (Ahr+/+) and AHR-null (Ahr-/-) mice. The Dp71 mRNA repression, caused by the βNF treatment, was also found in the liver tissue of wild type mice; however, such negative effect was reversed in the liver of AHR-null mice, which supports the participation of the Ahr signaling in Dp71 downregulation. Modulation of Dp71 expression by βNF may represent a novel mechanism of Ahr action. {\circledC} 2006 Elsevier B.V. All rights reserved.",
author = "{Berm{\'u}dez de Le{\'o}n}, Mario and Pablo G{\'o}mez and Guillermo Elizondo and Roc{\'i}o Zatarain-Palacios and Francisco Garc{\'i}a-Sierra and Bulmaro Cisneros",
year = "2006",
month = "3",
day = "1",
doi = "10.1016/j.bbaexp.2006.03.005",
language = "English",
pages = "152--158",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
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β-naphthoflavone represses dystrophin Dp71 expression in Hepa-1 cells. / Bermúdez de León, Mario; Gómez, Pablo; Elizondo, Guillermo; Zatarain-Palacios, Rocío; García-Sierra, Francisco; Cisneros, Bulmaro.

En: Biochimica et Biophysica Acta - Gene Structure and Expression, 01.03.2006, p. 152-158.

Resultado de la investigación

TY - JOUR

T1 - β-naphthoflavone represses dystrophin Dp71 expression in Hepa-1 cells

AU - Bermúdez de León, Mario

AU - Gómez, Pablo

AU - Elizondo, Guillermo

AU - Zatarain-Palacios, Rocío

AU - García-Sierra, Francisco

AU - Cisneros, Bulmaro

PY - 2006/3/1

Y1 - 2006/3/1

N2 - Dystrophin Dp71 is expressed in hepatic tissue; however, its function in this tissue remains unknown. The Dp71 promoter sequence contains conserved CACGC motifs, which constitute the invariant core sequence of xenobiotic-regulatory elements. These elements function as target sites for the aryl hydrocarbon receptor/aryl hydrocarbon nuclear translocator (Ahr/ARNT) in genes regulated by this transcription factor. Thus, Dp71 expression in hepatic cells would be regulated by Ahr signaling. In this study, the effect of the xenobiotics β-Naphthoflavone (βNF), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and Benzo[a]Pyrene (BaP) on Dp71 expression was analyzed in Hepa-1 cells. It was demonstrated that βNF, but not BaP or TCDD, represses Dp71 expression at both transcriptional and translational levels. To test directly the involvement of the Ahr signaling in the negative regulation of Dp71, we analyzed the effect of βNF on Dp71 expression in the liver of wild type (Ahr+/+) and AHR-null (Ahr-/-) mice. The Dp71 mRNA repression, caused by the βNF treatment, was also found in the liver tissue of wild type mice; however, such negative effect was reversed in the liver of AHR-null mice, which supports the participation of the Ahr signaling in Dp71 downregulation. Modulation of Dp71 expression by βNF may represent a novel mechanism of Ahr action. © 2006 Elsevier B.V. All rights reserved.

AB - Dystrophin Dp71 is expressed in hepatic tissue; however, its function in this tissue remains unknown. The Dp71 promoter sequence contains conserved CACGC motifs, which constitute the invariant core sequence of xenobiotic-regulatory elements. These elements function as target sites for the aryl hydrocarbon receptor/aryl hydrocarbon nuclear translocator (Ahr/ARNT) in genes regulated by this transcription factor. Thus, Dp71 expression in hepatic cells would be regulated by Ahr signaling. In this study, the effect of the xenobiotics β-Naphthoflavone (βNF), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and Benzo[a]Pyrene (BaP) on Dp71 expression was analyzed in Hepa-1 cells. It was demonstrated that βNF, but not BaP or TCDD, represses Dp71 expression at both transcriptional and translational levels. To test directly the involvement of the Ahr signaling in the negative regulation of Dp71, we analyzed the effect of βNF on Dp71 expression in the liver of wild type (Ahr+/+) and AHR-null (Ahr-/-) mice. The Dp71 mRNA repression, caused by the βNF treatment, was also found in the liver tissue of wild type mice; however, such negative effect was reversed in the liver of AHR-null mice, which supports the participation of the Ahr signaling in Dp71 downregulation. Modulation of Dp71 expression by βNF may represent a novel mechanism of Ahr action. © 2006 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.bbaexp.2006.03.005

DO - 10.1016/j.bbaexp.2006.03.005

M3 - Article

SP - 152

EP - 158

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

ER -