“Expresión de los genes que codifican para los transportadores de glucosa en células de Yarrowia lipolytica: GPA1 y GPA2”

Abstract

In higher and lower eukaryotic organisms, the PKA pathway is particularly relevant in glucose sensing; this pathway plays a central role in metabolism and cellular homeostasis, enabling various biological processes to be carried out. As this is a conserved signaling pathway in both higher and lower eukaryotic organisms, Yarrowia lipolytica cells have been used to study the PKA pathway. In previous laboratory studies using Y. lipolytica as a model organism, it was observed that inactivating the PKA pathway by deleting the genes encoding the α subunit (GPA1 and GPA2) results in greater proliferation of cells using glucose as a carbon source; however, such cells exhibit faster senescence.
In the present work, cells from the double mutant strain gpa1-𝚫/gpa2-𝚫 created by Briones (2021) and cells from the parental strain P01a as a control were used, and the expression pattern of the YHT (1 to 6) genes identified in Y. lipolytica, which encode hexose transporters, was analyzed to determine whether the alteration of glucose sensing by blocking the PKA pathway modifies the expression of these genes, which could explain the previously reported findings.
The expression of the genes encoding hexose transporters was analyzed by RT-PCR, and it was found that each gene shows a distinct expression pattern in both the parental strain and the double mutant strain gpa1-𝚫/gpa2-𝚫
Upon exposing the control and double mutant cells to glucose, the expression of YHT1, YHT4, and YHT5 decreased, while for YHT3 and YHT6, this phenomenon was only observed in the control cells but not in the gpa1-𝚫/gpa2-𝚫 cells. In contrast, the YHT2 gene did not show expression under any treatment in either the control strain or the double mutant strain.

Date of Award	4 Dec 2024
Original language	Spanish
Awarding Institution	Universidad de Monterrey