“Efecto de la cafeína en la regulación transcripcional de la respuesta antioxidante en células de Yarrowia lipolytica”

Abstract

Oxidative stress (OS) is a condition caused by an excessive presence of reactive oxygen species (ROS) and an insufficient amount of antioxidant mechanisms (enzymatic and non-enzymatic) to counteract it, promoting the development of chronic-degenerative diseases. Caffeine can stimulate the expression of genes that encode catalase, superoxide dismutase, and glutathione peroxidase enzymes (cellular antioxidant response) in the model organism Yarrowia lipolytica; however, the regulatory mechanisms of their expression remain unknown. Transcription factors (TFs) such as Yap1, Skn7, Msn2, and Msn4 regulate the expression of antioxidant genes under different stress conditions in various yeast species; although, the mechanism regulating the antioxidant response stimulated by caffeine is not yet understood. To verify the role played by different TFs in response to caffeine, cells of the P01a strain of Y. lipolytica yeast were subjected to different types of stress (oxidative, thermal, osmotic, caffeine exposure), and the expression pattern of the genes encoding the mentioned TFs was analyzed.
The results obtained show no differences in the expression of any of the genes (one-way ANOVA test) in cells subjected to the different types of stress, compared to the expression of genes in the control group cells (cells cultured in YPD medium). These findings suggest that caffeine does not modify the expression pattern of the genes corresponding to the TFs (Yap1, Skn7, Msn2, and Msn4).

Date of Award	4 Dec 2024
Original language	Spanish
Awarding Institution	Universidad de Monterrey