Desarrollo de circuitos neuronales en el sistema nervioso de ratones transgénicos EBF2-TGFP.

Translated title of the thesis: The development of neural circuits in the central nervous system of transgenic EBF"-TGFP mice.
  • María Blanca Delia Cepeda Varela
  • Lorena Melissa Cabrera Alvarado
  • Alma Cecilia Barboza Chávez
  • Román Vidaltamayo (Supervisor)

    Student thesis: Thesis

    Abstract

    The functions of the nervous system depend on the correct connection between neurons and the formation of circuits between them. The perception of the environment, decision-making, motivated behaviors and even remembering events, results from the activity of these circuits. However, the molecular mechanisms involved in the formation of these circuits and how they are organized are unknown.
    In this project we have identified that the EBF2 transcription factor is abundantly expressed in early stages of development in different areas of the murine nervous system.
    We use the GFP marker fused with a Tau protein, which is expressed under the control of the EBF2 promoter in transgenic mice. With this neuronal marker, the development of the formation of circuits that express it can be observed in different stages of embryonic and early postnatal development in the nervous system of the animal.
    EBF2 and its promoter are expressed in different areas of the nervous system, which are involved with the somatosensory and motivational and reward systems. Our results show its expression in the ventral posterior medial nucleus of the thalamus (VPM), in certain components of the trigeminal system, in the parabrachial nucleus and gelatinous substance of the spine, in the habenula (Hb), the medullary stria (sm), the bed of the stria terminalis (BNST), into the dorsal tegmental nucleus (DTg), and axons from the medial forebrain bundle through the lateral hypothalamus.
    More specifically: it is possible to map the entire trigeminothalamic tract from its origin at the bridge in the main trigeminal nucleus to the MPV of the contralateral thalamus, from intermediate embryonic stages to early postnatal stages (P21). In the same way, we can follow the projection of the DDCS circuit from the neurons in the BNST to the Hb, where they are projected towards the IPN through the RF, all this using a single marker.
    Date of Award20 May 2020
    Original languageSpanish
    Awarding Institution
    • Universidad de Monterrey
    SupervisorRomán Vidaltamayo (Supervisor)

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