Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP

M. González del Pliego, E. Aguirre-Benítez, M. Del Carmen Sánchez-Soto, M. Larrieta, A. Velázquez-Carranza, R. Vidaltamayo, M. Meza-Mora, A. Zepeda-Rodríguez, J. Hernández-Falcón, M. Hiriart

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Nerve growth factor (NGF) induces morphological and physiological changes in cultured pancreatic β-cells, including the extension of neurite-like processes. This latter effect is potentiated by dibutyryl cAMP (dbcAMP). β-cells cultured under these conditions maintain their immunoreactivity to insulin and gamma-amino-butyric acid (GABA). NGF, dbcAMP, and high glucose concentrations also increase the expression of the GABA-synthesizing enzyme glutamic acid decarboxylase-65 in cultured β-cells. The aim of this work was to study the effect of NGF alone or in combination with dbcAMP on pancreatic β-cell ultrastructural morphology, after 10 days in culture. We used light microscopy, scanning electron microscopy, and transmission electron microscopy to analyze the modifications in cell surface and neurite-like projections. Morphometric analysis showed that NGF and/or dbcAMP treatment substantially increased the insulin and GABA content in granules and rough endoplasmic reticulum. Given that pancreatic β-cells express NGF receptors and that NGF is synthesized and secreted by β-cells, these results further suggest that NGF could have trophic actions on pancreatic hormone synthesis and/or storage.
Original languageEnglish
Pages (from-to)365-378
Number of pages14
JournalCell and Tissue Research
DOIs
Publication statusPublished - 1 Dec 2001
Externally publishedYes

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Insulin-Secreting Cells
Nerve Growth Factor
Butyric Acid
Cultured Cells
Neurites
Pancreatic Hormones
Insulin
Nerve Growth Factor Receptors
Glutamate Decarboxylase
Rough Endoplasmic Reticulum
Transmission Electron Microscopy
Electron Scanning Microscopy
Microscopy
Light
Glucose
Enzymes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

González del Pliego, M., Aguirre-Benítez, E., Del Carmen Sánchez-Soto, M., Larrieta, M., Velázquez-Carranza, A., Vidaltamayo, R., ... Hiriart, M. (2001). Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP. Cell and Tissue Research, 365-378. https://doi.org/10.1007/s004410100411
González del Pliego, M. ; Aguirre-Benítez, E. ; Del Carmen Sánchez-Soto, M. ; Larrieta, M. ; Velázquez-Carranza, A. ; Vidaltamayo, R. ; Meza-Mora, M. ; Zepeda-Rodríguez, A. ; Hernández-Falcón, J. ; Hiriart, M. / Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP. In: Cell and Tissue Research. 2001 ; pp. 365-378.
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abstract = "Nerve growth factor (NGF) induces morphological and physiological changes in cultured pancreatic β-cells, including the extension of neurite-like processes. This latter effect is potentiated by dibutyryl cAMP (dbcAMP). β-cells cultured under these conditions maintain their immunoreactivity to insulin and gamma-amino-butyric acid (GABA). NGF, dbcAMP, and high glucose concentrations also increase the expression of the GABA-synthesizing enzyme glutamic acid decarboxylase-65 in cultured β-cells. The aim of this work was to study the effect of NGF alone or in combination with dbcAMP on pancreatic β-cell ultrastructural morphology, after 10 days in culture. We used light microscopy, scanning electron microscopy, and transmission electron microscopy to analyze the modifications in cell surface and neurite-like projections. Morphometric analysis showed that NGF and/or dbcAMP treatment substantially increased the insulin and GABA content in granules and rough endoplasmic reticulum. Given that pancreatic β-cells express NGF receptors and that NGF is synthesized and secreted by β-cells, these results further suggest that NGF could have trophic actions on pancreatic hormone synthesis and/or storage.",
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González del Pliego, M, Aguirre-Benítez, E, Del Carmen Sánchez-Soto, M, Larrieta, M, Velázquez-Carranza, A, Vidaltamayo, R, Meza-Mora, M, Zepeda-Rodríguez, A, Hernández-Falcón, J & Hiriart, M 2001, 'Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP', Cell and Tissue Research, pp. 365-378. https://doi.org/10.1007/s004410100411

Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP. / González del Pliego, M.; Aguirre-Benítez, E.; Del Carmen Sánchez-Soto, M.; Larrieta, M.; Velázquez-Carranza, A.; Vidaltamayo, R.; Meza-Mora, M.; Zepeda-Rodríguez, A.; Hernández-Falcón, J.; Hiriart, M.

In: Cell and Tissue Research, 01.12.2001, p. 365-378.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP

AU - González del Pliego, M.

AU - Aguirre-Benítez, E.

AU - Del Carmen Sánchez-Soto, M.

AU - Larrieta, M.

AU - Velázquez-Carranza, A.

AU - Vidaltamayo, R.

AU - Meza-Mora, M.

AU - Zepeda-Rodríguez, A.

AU - Hernández-Falcón, J.

AU - Hiriart, M.

PY - 2001/12/1

Y1 - 2001/12/1

N2 - Nerve growth factor (NGF) induces morphological and physiological changes in cultured pancreatic β-cells, including the extension of neurite-like processes. This latter effect is potentiated by dibutyryl cAMP (dbcAMP). β-cells cultured under these conditions maintain their immunoreactivity to insulin and gamma-amino-butyric acid (GABA). NGF, dbcAMP, and high glucose concentrations also increase the expression of the GABA-synthesizing enzyme glutamic acid decarboxylase-65 in cultured β-cells. The aim of this work was to study the effect of NGF alone or in combination with dbcAMP on pancreatic β-cell ultrastructural morphology, after 10 days in culture. We used light microscopy, scanning electron microscopy, and transmission electron microscopy to analyze the modifications in cell surface and neurite-like projections. Morphometric analysis showed that NGF and/or dbcAMP treatment substantially increased the insulin and GABA content in granules and rough endoplasmic reticulum. Given that pancreatic β-cells express NGF receptors and that NGF is synthesized and secreted by β-cells, these results further suggest that NGF could have trophic actions on pancreatic hormone synthesis and/or storage.

AB - Nerve growth factor (NGF) induces morphological and physiological changes in cultured pancreatic β-cells, including the extension of neurite-like processes. This latter effect is potentiated by dibutyryl cAMP (dbcAMP). β-cells cultured under these conditions maintain their immunoreactivity to insulin and gamma-amino-butyric acid (GABA). NGF, dbcAMP, and high glucose concentrations also increase the expression of the GABA-synthesizing enzyme glutamic acid decarboxylase-65 in cultured β-cells. The aim of this work was to study the effect of NGF alone or in combination with dbcAMP on pancreatic β-cell ultrastructural morphology, after 10 days in culture. We used light microscopy, scanning electron microscopy, and transmission electron microscopy to analyze the modifications in cell surface and neurite-like projections. Morphometric analysis showed that NGF and/or dbcAMP treatment substantially increased the insulin and GABA content in granules and rough endoplasmic reticulum. Given that pancreatic β-cells express NGF receptors and that NGF is synthesized and secreted by β-cells, these results further suggest that NGF could have trophic actions on pancreatic hormone synthesis and/or storage.

U2 - 10.1007/s004410100411

DO - 10.1007/s004410100411

M3 - Article

SP - 365

EP - 378

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

ER -

González del Pliego M, Aguirre-Benítez E, Del Carmen Sánchez-Soto M, Larrieta M, Velázquez-Carranza A, Vidaltamayo R et al. Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP. Cell and Tissue Research. 2001 Dec 1;365-378. https://doi.org/10.1007/s004410100411