TY - JOUR
T1 - Trichomonas vaginalis: Identification of soluble and membrane-associated phospholipase A1 and A2 activities with direct and indirect hemolytic effects
AU - Vargas-Villarreal, Javier
AU - Mata-Cárdenas, Benito David
AU - Palacios-Corona, Rebeca
AU - González-Salazar, Francisco
AU - Cortes-Gutierrez, Elva I.
AU - Martínez-Rodríguez, Herminia G.
AU - Said-Fernández, Salvador
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/2/1
Y1 - 2005/2/1
N2 - A direct hemolytic activity, dependent on phospholipase A (PLA) activity, was located in the particulate subcellular fraction (P30) of Trichomonas vaginalis. We identified soluble direct and indirect hemolytic activities in the spent medium and soluble fraction (S30) of T. vaginalis strain GT-13. Spent medium showed the highest specific indirect hemolytic activity (SIHA) at pH 6.0 (91 indirect hemolytic units [HU]/mg/hr). Spent medium and P30, but not S30, showed direct hemolytic activity. PLA activity was protein dose dependent and time dependent. The highest PLA activity was observed at pH 6.0. All trichomonad preparations showed phospholipase A
1 (PLA A
1) and phospholipase A
2 (PLA A
2) activities. Indirect and direct hemolytic activity and PLA A
1 and PLA A
2 diminished at pH 6.0 and 8.0 with increasing concentrations of Rosenthal's inhibitor. The greatest effect was observed with 80 μM at pH 6.0 on the SIHA of S30 (83% reduction) and the lowest at pH 8.0, also on the SIHA of S30 (26% reduction). In conclusion, T. vaginalis contains particulate and soluble acidic, and alkaline direct and indirect hemolytic activities, which are partially dependent on alkaline or acidic PLA A
1 and PLA A
2 enzymes. These could be responsible for the contact-dependent and -independent hemolytic and cytolytic activities of T. vaginalis.
AB - A direct hemolytic activity, dependent on phospholipase A (PLA) activity, was located in the particulate subcellular fraction (P30) of Trichomonas vaginalis. We identified soluble direct and indirect hemolytic activities in the spent medium and soluble fraction (S30) of T. vaginalis strain GT-13. Spent medium showed the highest specific indirect hemolytic activity (SIHA) at pH 6.0 (91 indirect hemolytic units [HU]/mg/hr). Spent medium and P30, but not S30, showed direct hemolytic activity. PLA activity was protein dose dependent and time dependent. The highest PLA activity was observed at pH 6.0. All trichomonad preparations showed phospholipase A
1 (PLA A
1) and phospholipase A
2 (PLA A
2) activities. Indirect and direct hemolytic activity and PLA A
1 and PLA A
2 diminished at pH 6.0 and 8.0 with increasing concentrations of Rosenthal's inhibitor. The greatest effect was observed with 80 μM at pH 6.0 on the SIHA of S30 (83% reduction) and the lowest at pH 8.0, also on the SIHA of S30 (26% reduction). In conclusion, T. vaginalis contains particulate and soluble acidic, and alkaline direct and indirect hemolytic activities, which are partially dependent on alkaline or acidic PLA A
1 and PLA A
2 enzymes. These could be responsible for the contact-dependent and -independent hemolytic and cytolytic activities of T. vaginalis.
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U2 - 10.1645/GE-3338
DO - 10.1645/GE-3338
M3 - Article
SN - 0022-3395
VL - 91
SP - 5
EP - 11
JO - Journal of Parasitology
JF - Journal of Parasitology
IS - 1
ER -