Trichomonas vaginalis and giardia lamblia growth alterations by low-frequency electromagnetic fields

Abraham Octavio RODRÍGUEZ-DE LA FUENTE, Ricardo Gomez-Flores, José Antonio Heredia-Rojas, Edna Marbella García-Muñoz, Javier Vargas-Villarreal, Magda Elizabeth Hernández-García, Francisco González-Salazar, Jesús Norberto Garza-González, Michaela Beltcheva, Omar Heredia-Rodríguez

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: There is an increasing interest in using physical factors such as magnetic fields as antimicrobial strategy, with variable results. The current study was aimed to evaluate the influence of extremely low-frequency electromagnetic fields (ELF-EMFs) on the axenically-cultured parasite protozoans Trichomonas vaginalis and Giardia lamblia growth. Methods: Bioassays were developed using T. vaginalis, GT-13 and G. lamblia IMSS-0989 strains cultured at 37 ºC in TYI-S-33 medium. The following treatment regimens and controls were considered: (a) cells exposed to ELF-EMFs, (b) untreated cells, (c) cells treated with Metronidazole, used as positive controls, and (d) cells co-exposed to ELF-EMFs and Metronidazole. When cultures reached the end of logarithmic phase, they were exposed to ELF-EMFs for 72 h, in a standardized magnetic field exposure facility. For determining cytotoxic effects, trophozoite density was blindly evaluated in a Neubauer chamber. Results: A significant decrease in trophozoite growth was observed for T. vaginalis, in magnetic field-treated cultures. On the other hand, cultures co-exposed to ELF-EMFs and Metronidazole showed no significant differences when compared with cultures treated with Metronidazole alone. On the contrary, an increased trophozoite density was observed in G. lamblia cultures after exposure to magnetic fields. An absence of a synergistic or antagonistic effect was observed. Conclusion: ELF-EMFs induced T. vaginalis and G. lamblia growth alterations, indicating a potential effect in cell cycle progression.

Original languageEnglish
Pages (from-to)652-656
Number of pages5
JournalIranian Journal of Parasitology
Volume14
Issue number4
DOIs
Publication statusPublished - 1 Oct 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019, Tehran University of Medical Sciences (TUMS). All rights reserved.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Trichomonas vaginalis and giardia lamblia growth alterations by low-frequency electromagnetic fields'. Together they form a unique fingerprint.

Cite this