Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells

Katia Peñuelas-Urquides, Carolina Becerril-Esquivel, Laura C. Mendoza-de-León, Beatriz Silva-Ramírez, José Dávila-Velderrain, Bulmaro Cisneros, Mario Bermúdez De León

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

© 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society. Dystrophin Dp71, the smallest product encoded by the Duchenne muscular dystrophy gene, is ubiquitously expressed in all nonmuscle cells. Although Dp71 is involved in various cellular processes, the mechanisms underlying its expression have been little studied. In hepatic cells, Dp71 expression is down-regulated by the xenobiotic β-naphthoflavone. However, the effectors of this regulation remain unknown. In the present study we aimed at identifying DNA elements and transcription factors involved in Dp71 expression in hepatic cells. Relevant DNA elements on the Dp71 promoter were identified by comparing Dp71 5′-end flanking regions between species. The functionality of these elements was demonstrated by site-directed mutagenesis. Using EMSAs and ChIP, we showed that the Sp1 (specificity protein 1), Sp3 (specificity protein 3) and YY1 (Yin and Yang 1) transcription factors bind to the Dp71 promoter region. Knockdown of Sp1, Sp3 and YY1 in hepatic cells increased endogenous Dp71 expression, but reduced Dp71 promoter activity. In summary, Dp71 expression in hepatic cells is carried out, in part, by YY1-, Sp1- and Sp3-mediated transcription from the Dp71 promoter.
Original languageEnglish
Pages (from-to)1967-1976
Number of pages10
JournalBiochemical Journal
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

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YY1 Transcription Factor
Dystrophin
Gene expression
Hepatocytes
Transcription Factors
Gene Expression
Proteins
Mutagenesis
Duchenne Muscular Dystrophy
5' Flanking Region
DNA
Xenobiotics
Transcription
Site-Directed Mutagenesis
Genetic Promoter Regions
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Peñuelas-Urquides, K., Becerril-Esquivel, C., Mendoza-de-León, L. C., Silva-Ramírez, B., Dávila-Velderrain, J., Cisneros, B., & Bermúdez De León, M. (2016). Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells. Biochemical Journal, 1967-1976. https://doi.org/10.1042/BCJ20160163
Peñuelas-Urquides, Katia ; Becerril-Esquivel, Carolina ; Mendoza-de-León, Laura C. ; Silva-Ramírez, Beatriz ; Dávila-Velderrain, José ; Cisneros, Bulmaro ; Bermúdez De León, Mario. / Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells. In: Biochemical Journal. 2016 ; pp. 1967-1976.
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Peñuelas-Urquides, K, Becerril-Esquivel, C, Mendoza-de-León, LC, Silva-Ramírez, B, Dávila-Velderrain, J, Cisneros, B & Bermúdez De León, M 2016, 'Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells', Biochemical Journal, pp. 1967-1976. https://doi.org/10.1042/BCJ20160163

Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells. / Peñuelas-Urquides, Katia; Becerril-Esquivel, Carolina; Mendoza-de-León, Laura C.; Silva-Ramírez, Beatriz; Dávila-Velderrain, José; Cisneros, Bulmaro; Bermúdez De León, Mario.

In: Biochemical Journal, 01.01.2016, p. 1967-1976.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells

AU - Peñuelas-Urquides, Katia

AU - Becerril-Esquivel, Carolina

AU - Mendoza-de-León, Laura C.

AU - Silva-Ramírez, Beatriz

AU - Dávila-Velderrain, José

AU - Cisneros, Bulmaro

AU - Bermúdez De León, Mario

PY - 2016/1/1

Y1 - 2016/1/1

N2 - © 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society. Dystrophin Dp71, the smallest product encoded by the Duchenne muscular dystrophy gene, is ubiquitously expressed in all nonmuscle cells. Although Dp71 is involved in various cellular processes, the mechanisms underlying its expression have been little studied. In hepatic cells, Dp71 expression is down-regulated by the xenobiotic β-naphthoflavone. However, the effectors of this regulation remain unknown. In the present study we aimed at identifying DNA elements and transcription factors involved in Dp71 expression in hepatic cells. Relevant DNA elements on the Dp71 promoter were identified by comparing Dp71 5′-end flanking regions between species. The functionality of these elements was demonstrated by site-directed mutagenesis. Using EMSAs and ChIP, we showed that the Sp1 (specificity protein 1), Sp3 (specificity protein 3) and YY1 (Yin and Yang 1) transcription factors bind to the Dp71 promoter region. Knockdown of Sp1, Sp3 and YY1 in hepatic cells increased endogenous Dp71 expression, but reduced Dp71 promoter activity. In summary, Dp71 expression in hepatic cells is carried out, in part, by YY1-, Sp1- and Sp3-mediated transcription from the Dp71 promoter.

AB - © 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society. Dystrophin Dp71, the smallest product encoded by the Duchenne muscular dystrophy gene, is ubiquitously expressed in all nonmuscle cells. Although Dp71 is involved in various cellular processes, the mechanisms underlying its expression have been little studied. In hepatic cells, Dp71 expression is down-regulated by the xenobiotic β-naphthoflavone. However, the effectors of this regulation remain unknown. In the present study we aimed at identifying DNA elements and transcription factors involved in Dp71 expression in hepatic cells. Relevant DNA elements on the Dp71 promoter were identified by comparing Dp71 5′-end flanking regions between species. The functionality of these elements was demonstrated by site-directed mutagenesis. Using EMSAs and ChIP, we showed that the Sp1 (specificity protein 1), Sp3 (specificity protein 3) and YY1 (Yin and Yang 1) transcription factors bind to the Dp71 promoter region. Knockdown of Sp1, Sp3 and YY1 in hepatic cells increased endogenous Dp71 expression, but reduced Dp71 promoter activity. In summary, Dp71 expression in hepatic cells is carried out, in part, by YY1-, Sp1- and Sp3-mediated transcription from the Dp71 promoter.

U2 - 10.1042/BCJ20160163

DO - 10.1042/BCJ20160163

M3 - Article

SP - 1967

EP - 1976

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

ER -

Peñuelas-Urquides K, Becerril-Esquivel C, Mendoza-de-León LC, Silva-Ramírez B, Dávila-Velderrain J, Cisneros B et al. Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells. Biochemical Journal. 2016 Jan 1;1967-1976. https://doi.org/10.1042/BCJ20160163