The inflammatory process modulates the expression and localization of WT1 in podocytes leading to kidney damage

Mariela Arellano-Rodriguez, Pablo Zapata-Benavides*, Norma Cesilia Arellano-Rodriguez, Juan Manuel Izaguirre-Alvarez, Moises Armides Franco-Molina, Felipe De Jesus Torres Del Muro, Edgar Mendoza-Gamboa, Adolfo Soto-Dominguez, Santiago Saavedra-Alonso, Cristina Rodriguez-Padilla

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Background/Aim: Wilms' tumor 1 (WT1) is involved in the development of the urogenital system and is expressed in podocytes throughout life. Inflammation of renal glomeruli causes renal damage-induced nephrotic syndrome and steroid-resistant nephrotic syndrome have mutations in the WT1 gene. The aim of this work was to determine if the inflammatory process modulates the expression and localization of WT1 in podocytes that cause kidney damage using lipopolysaccharide (LPS)-treated mice as a sepsis model. Materials and Methods: In investigation of renal damage, proteinuria and histology were analyzed. WT1 modulation was analyzed by indirect immunofluorescence, immunohistochemistry and western blot assays, and proinflammatory cytokines were analyzed by quantitative polymerase chain reaction assay. Results: WT1 expression decreased most at 24 and 36 h after the induction of inflammation and phosphorylated WT1 was mainly localized in the cytoplasm, reduced nephrin mRNA expression and increased mRNA expression of tumor necrosis factor a and interleukin 1β. Conclusion: These results indicate that the immune system plays an important role in the modulation of WT1, leading to kidney damage.

Original languageEnglish
Pages (from-to)3137-3146
Number of pages10
JournalIn Vivo
Volume35
Issue number6
DOIs
Publication statusPublished - Dec 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2021 International Institute of Anticancer Research. All rights reserved.

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology
  • Pharmacology

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