The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population

Baltazar González-Andrade, Ramiro Santos-Lartigue, Samantha Flores-Treviño, Natalie Sofia Ramirez-Ochoa, Paola Bocanegra-Ibarias, Francisco J Huerta-Torres, Soraya Mendoza-Olazarán, Licet Villarreal-Treviño, Adrián Camacho-Ortiz, Hipólito Villarreal-Vázquez, Elvira Garza-González

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Abstract

The aim of the present study was to estimate the relative contribution of immunogenetic and microbiological factors in the development of recurrent tonsillitis in a Mexican population. Patients (n = 138) with recurrent tonsillitis and an indication of tonsillectomy (mean age: 6.05 years ± 3.00; median age: 5 years, female: 58; age range: 1-15 years) and 195 non-related controls older than 18 years and a medical history free of recurrent tonsillitis were included. To evaluate the microbial contribution, tonsil swab samples from both groups and extracted tonsil samples from cases were cultured. Biofilm production of isolated bacteria was measured. To assess the immunogenetic component, DNA from peripheral blood was genotyped for the TNFA-308G/A single-nucleotide polymorphism (SNP) and for the IL1B -31C/T SNP. Normal microbiota, but no pathogens or potential pathogens, were identified from all control sample cultures. The most frequent pathogenic species detected in tonsils from cases were Staphylococcus aureus (48.6%, 67/138) and Haemophilus influenzae (31.9%, 44/138), which were found more frequently in patient samples than in samples from healthy volunteers (P < 0.0001). Importantly, 41/54 (75.9%) S. aureus isolates were biofilm producers (18 weak and 23 strong), whereas 17/25 (68%) H. influenzae isolates were biofilm producers (10 weak, and 7 strong biofilm producers). Patients with at least one copy of the IL1B-31*C allele had a higher risk of recurrent tonsillitis (OR = 4.03; 95% CI = 1.27-14.27; P = 0.013). TNFA-308 G/A alleles were not preferentially distributed among the groups. When considering the presence of IL1B-31*C plus S. aureus, IL1B-31*C plus S. aureus biofilm producer, IL1B-31*C plus H. influenzae or IL1B-31*C plus H. influenzae biofilm producer, the OR tended to infinite. Thus, the presence of IL1B-31*C allele plus the presence of S. aureus and/or H. influenzae could be related to the development of tonsillitis in this particular Mexican population.

Original languageEnglish
Pages (from-to)e0178115
JournalPLoS One
Volume12
Issue number5
DOIs
Publication statusPublished - 2017
Externally publishedYes

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Haemophilus influenzae
Tonsillitis
Interleukins
Biofilms
interleukins
biofilm
Staphylococcus aureus
Alleles
alleles
tonsils
Palatine Tonsil
immunogenetics
Population
Immunogenetics
Pathogens
Polymorphism
single nucleotide polymorphism
Single Nucleotide Polymorphism
sampling
Nucleotides

Cite this

González-Andrade, B., Santos-Lartigue, R., Flores-Treviño, S., Ramirez-Ochoa, N. S., Bocanegra-Ibarias, P., Huerta-Torres, F. J., ... Garza-González, E. (2017). The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population. PLoS One, 12(5), e0178115. https://doi.org/10.1371/journal.pone.0178115
González-Andrade, Baltazar ; Santos-Lartigue, Ramiro ; Flores-Treviño, Samantha ; Ramirez-Ochoa, Natalie Sofia ; Bocanegra-Ibarias, Paola ; Huerta-Torres, Francisco J ; Mendoza-Olazarán, Soraya ; Villarreal-Treviño, Licet ; Camacho-Ortiz, Adrián ; Villarreal-Vázquez, Hipólito ; Garza-González, Elvira. / The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population. In: PLoS One. 2017 ; Vol. 12, No. 5. pp. e0178115.
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abstract = "The aim of the present study was to estimate the relative contribution of immunogenetic and microbiological factors in the development of recurrent tonsillitis in a Mexican population. Patients (n = 138) with recurrent tonsillitis and an indication of tonsillectomy (mean age: 6.05 years ± 3.00; median age: 5 years, female: 58; age range: 1-15 years) and 195 non-related controls older than 18 years and a medical history free of recurrent tonsillitis were included. To evaluate the microbial contribution, tonsil swab samples from both groups and extracted tonsil samples from cases were cultured. Biofilm production of isolated bacteria was measured. To assess the immunogenetic component, DNA from peripheral blood was genotyped for the TNFA-308G/A single-nucleotide polymorphism (SNP) and for the IL1B -31C/T SNP. Normal microbiota, but no pathogens or potential pathogens, were identified from all control sample cultures. The most frequent pathogenic species detected in tonsils from cases were Staphylococcus aureus (48.6{\%}, 67/138) and Haemophilus influenzae (31.9{\%}, 44/138), which were found more frequently in patient samples than in samples from healthy volunteers (P < 0.0001). Importantly, 41/54 (75.9{\%}) S. aureus isolates were biofilm producers (18 weak and 23 strong), whereas 17/25 (68{\%}) H. influenzae isolates were biofilm producers (10 weak, and 7 strong biofilm producers). Patients with at least one copy of the IL1B-31*C allele had a higher risk of recurrent tonsillitis (OR = 4.03; 95{\%} CI = 1.27-14.27; P = 0.013). TNFA-308 G/A alleles were not preferentially distributed among the groups. When considering the presence of IL1B-31*C plus S. aureus, IL1B-31*C plus S. aureus biofilm producer, IL1B-31*C plus H. influenzae or IL1B-31*C plus H. influenzae biofilm producer, the OR tended to infinite. Thus, the presence of IL1B-31*C allele plus the presence of S. aureus and/or H. influenzae could be related to the development of tonsillitis in this particular Mexican population.",
author = "Baltazar Gonz{\'a}lez-Andrade and Ramiro Santos-Lartigue and Samantha Flores-Trevi{\~n}o and Ramirez-Ochoa, {Natalie Sofia} and Paola Bocanegra-Ibarias and Huerta-Torres, {Francisco J} and Soraya Mendoza-Olazar{\'a}n and Licet Villarreal-Trevi{\~n}o and Adri{\'a}n Camacho-Ortiz and Hip{\'o}lito Villarreal-V{\'a}zquez and Elvira Garza-Gonz{\'a}lez",
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González-Andrade, B, Santos-Lartigue, R, Flores-Treviño, S, Ramirez-Ochoa, NS, Bocanegra-Ibarias, P, Huerta-Torres, FJ, Mendoza-Olazarán, S, Villarreal-Treviño, L, Camacho-Ortiz, A, Villarreal-Vázquez, H & Garza-González, E 2017, 'The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population', PLoS One, vol. 12, no. 5, pp. e0178115. https://doi.org/10.1371/journal.pone.0178115

The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population. / González-Andrade, Baltazar; Santos-Lartigue, Ramiro; Flores-Treviño, Samantha; Ramirez-Ochoa, Natalie Sofia; Bocanegra-Ibarias, Paola; Huerta-Torres, Francisco J; Mendoza-Olazarán, Soraya; Villarreal-Treviño, Licet; Camacho-Ortiz, Adrián; Villarreal-Vázquez, Hipólito; Garza-González, Elvira.

In: PLoS One, Vol. 12, No. 5, 2017, p. e0178115.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population

AU - González-Andrade, Baltazar

AU - Santos-Lartigue, Ramiro

AU - Flores-Treviño, Samantha

AU - Ramirez-Ochoa, Natalie Sofia

AU - Bocanegra-Ibarias, Paola

AU - Huerta-Torres, Francisco J

AU - Mendoza-Olazarán, Soraya

AU - Villarreal-Treviño, Licet

AU - Camacho-Ortiz, Adrián

AU - Villarreal-Vázquez, Hipólito

AU - Garza-González, Elvira

PY - 2017

Y1 - 2017

N2 - The aim of the present study was to estimate the relative contribution of immunogenetic and microbiological factors in the development of recurrent tonsillitis in a Mexican population. Patients (n = 138) with recurrent tonsillitis and an indication of tonsillectomy (mean age: 6.05 years ± 3.00; median age: 5 years, female: 58; age range: 1-15 years) and 195 non-related controls older than 18 years and a medical history free of recurrent tonsillitis were included. To evaluate the microbial contribution, tonsil swab samples from both groups and extracted tonsil samples from cases were cultured. Biofilm production of isolated bacteria was measured. To assess the immunogenetic component, DNA from peripheral blood was genotyped for the TNFA-308G/A single-nucleotide polymorphism (SNP) and for the IL1B -31C/T SNP. Normal microbiota, but no pathogens or potential pathogens, were identified from all control sample cultures. The most frequent pathogenic species detected in tonsils from cases were Staphylococcus aureus (48.6%, 67/138) and Haemophilus influenzae (31.9%, 44/138), which were found more frequently in patient samples than in samples from healthy volunteers (P < 0.0001). Importantly, 41/54 (75.9%) S. aureus isolates were biofilm producers (18 weak and 23 strong), whereas 17/25 (68%) H. influenzae isolates were biofilm producers (10 weak, and 7 strong biofilm producers). Patients with at least one copy of the IL1B-31*C allele had a higher risk of recurrent tonsillitis (OR = 4.03; 95% CI = 1.27-14.27; P = 0.013). TNFA-308 G/A alleles were not preferentially distributed among the groups. When considering the presence of IL1B-31*C plus S. aureus, IL1B-31*C plus S. aureus biofilm producer, IL1B-31*C plus H. influenzae or IL1B-31*C plus H. influenzae biofilm producer, the OR tended to infinite. Thus, the presence of IL1B-31*C allele plus the presence of S. aureus and/or H. influenzae could be related to the development of tonsillitis in this particular Mexican population.

AB - The aim of the present study was to estimate the relative contribution of immunogenetic and microbiological factors in the development of recurrent tonsillitis in a Mexican population. Patients (n = 138) with recurrent tonsillitis and an indication of tonsillectomy (mean age: 6.05 years ± 3.00; median age: 5 years, female: 58; age range: 1-15 years) and 195 non-related controls older than 18 years and a medical history free of recurrent tonsillitis were included. To evaluate the microbial contribution, tonsil swab samples from both groups and extracted tonsil samples from cases were cultured. Biofilm production of isolated bacteria was measured. To assess the immunogenetic component, DNA from peripheral blood was genotyped for the TNFA-308G/A single-nucleotide polymorphism (SNP) and for the IL1B -31C/T SNP. Normal microbiota, but no pathogens or potential pathogens, were identified from all control sample cultures. The most frequent pathogenic species detected in tonsils from cases were Staphylococcus aureus (48.6%, 67/138) and Haemophilus influenzae (31.9%, 44/138), which were found more frequently in patient samples than in samples from healthy volunteers (P < 0.0001). Importantly, 41/54 (75.9%) S. aureus isolates were biofilm producers (18 weak and 23 strong), whereas 17/25 (68%) H. influenzae isolates were biofilm producers (10 weak, and 7 strong biofilm producers). Patients with at least one copy of the IL1B-31*C allele had a higher risk of recurrent tonsillitis (OR = 4.03; 95% CI = 1.27-14.27; P = 0.013). TNFA-308 G/A alleles were not preferentially distributed among the groups. When considering the presence of IL1B-31*C plus S. aureus, IL1B-31*C plus S. aureus biofilm producer, IL1B-31*C plus H. influenzae or IL1B-31*C plus H. influenzae biofilm producer, the OR tended to infinite. Thus, the presence of IL1B-31*C allele plus the presence of S. aureus and/or H. influenzae could be related to the development of tonsillitis in this particular Mexican population.

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DO - 10.1371/journal.pone.0178115

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SP - e0178115

JO - PLoS One

JF - PLoS One

SN - 1932-6203

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González-Andrade B, Santos-Lartigue R, Flores-Treviño S, Ramirez-Ochoa NS, Bocanegra-Ibarias P, Huerta-Torres FJ et al. The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population. PLoS One. 2017;12(5):e0178115. https://doi.org/10.1371/journal.pone.0178115