Synergistic antitumor activity of gramicidin/lipophilic bismuth nanoparticles (BisBAL NPs) on human cervical tumor cells

Claudio Cabral-Romero, Claudia María García-Cuellar, Rene Hernandez-Delgadillo, Yesennia Sánchez-Pérez, Irene Meester, Juan Manuel Solís-Soto, Nayely Pineda-Aguilar, Shankararaman Chellam

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3 Citations (Scopus)


The objective of this study was to study the synergistic antitumor effect of lipophilic bismuth nanoparticles (BisBAL NPs) with the antibiotic solution Neo-Poly gramicidin on human cervical tumor cells. The effect of BisBAL NPs and Neo-Poly gramicidin solution on cervical cancer cell line (HeLa) was determined by the MTT cell viability assay and fluorescence microscopy. After a 24-h exposure to 0.1× Neo-Poly gramicidin HeLa cell growth decreased 94%. Fluorescence microscopy confirmed the antitumor effect cell death was higher among treated than among non-treated cells cells. Individually, gramicidin (0.04 mg/mL) inhibited HeLa tumor cell growth most (40%), and neomycin (0.04 mg/mL) least (21%). Gramicidin (0.3 mg/mL) in combination with different concentrations (1–150 μM) of BisBAL NPs had a synergistic antitumor effect against HeLa cells, reaching an < 86% tumor growth inhibition. As far as we know, we are the first to describe the antitumor activity of the antibiotic Neo-Poly gramicidin on a human cervical cancer cell line. The action mechanism of gramicidin/BisBAL NP is based on a strong damage on cell membrane and nucleus of tumor cells. A synergistic effect of gramicidin with BisBAL NPs may be useful as an alternative therapy for cervical cancer patients.

Original languageEnglish
Article number633604
Number of pages10
JournalFrontiers in Nanotechnology
Publication statusPublished - 12 Apr 2021

Bibliographical note

Funding Information:
The authors thank the Sectorial Fund for Education Research, CONACyT, CB 2017-2018 for grant A1-S-20148.

Publisher Copyright:
Copyright © 2021 Cabral-Romero, García-Cuellar, Hernandez-Delgadillo, Sánchez-Pérez, Meester, Solís-Soto, Pineda-Aguilar and Chellam.

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Biomedical Engineering
  • Computer Science Applications


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