Stenotrophomonas maltophilia in Mexico: antimicrobial resistance, biofilm formation and clonal diversity

Samantha Flores-Treviño, Jessica Lizzeth Gutiérrez-Ferman, Rayo Morfín-Otero, Eduardo Rodríguez-Noriega, Diego Estrada-Rivadeneyra, Catalina Rivas-Morales, Jorge M Llaca-Díaz, Adrián Camacho-Ortíz, Soraya Mendoza-Olazarán, Elvira Garza-González

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14 Citations (Scopus)

Abstract

Stenotrophomonas maltophilia is an important multidrug-resistant nosocomial pathogen associated with high mortality. Our aim was to examine antimicrobial susceptibility, biofilm production and clonal relatedness of clinical isolates of S. maltophilia. S. maltophilia isolates were collected between 2006 and 2013 from two tertiary care hospitals in Mexico. Antimicrobial susceptibility was evaluated by the broth microdilution method. PCR was used to determine the presence of β-lactamase genes L1 and L2. Biofilm formation was assessed with crystal violet staining. Clonal relatedness was determined by PFGE. Among the 119 collected S. maltophilia isolates, 73 (61.3%) were from the respiratory tract. Resistance levels exceeded 75% for imipenem, meropenem, ampicillin, aztreonam, gentamicin and tobramycin. Resistance to trimethoprim-sulfamethoxazole was 32.8%. L1 and L2 genes were detected in 77.1% (91/118) and 66.9% (79/118) of isolates, respectively. All S. maltophilia strains were able to produce biofilms. Strains were classified as weak (47.9%, 57/119), moderate (38.7%, 46/119), or strong (13.4%, 16/119) biofilm producers. A total of 89 distinct PFGE types were identified and 21.6% (22/102) of the isolates were distributed in nine clusters. This is the first study in Mexico to reveal characteristics of clinical isolates of S. maltophilia. Clonal diversity data indicate low cross-transmission of S. maltophilia in a hospital setting. The high antibiotic resistance underscores the need for continuous surveillance of S. maltophilia in hospital settings in Mexico.

Original languageEnglish
Pages (from-to)1524-30
Number of pages7
JournalJournal of Medical Microbiology
Volume63
Issue numberPt 11
DOIs
Publication statusPublished - Nov 2014
Externally publishedYes

Bibliographical note

© 2014 The Authors.

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    Flores-Treviño, S., Gutiérrez-Ferman, J. L., Morfín-Otero, R., Rodríguez-Noriega, E., Estrada-Rivadeneyra, D., Rivas-Morales, C., Llaca-Díaz, J. M., Camacho-Ortíz, A., Mendoza-Olazarán, S., & Garza-González, E. (2014). Stenotrophomonas maltophilia in Mexico: antimicrobial resistance, biofilm formation and clonal diversity. Journal of Medical Microbiology, 63(Pt 11), 1524-30. https://doi.org/10.1099/jmm.0.074385-0