Stenotrophomonas maltophilia in Mexico: antimicrobial resistance, biofilm formation and clonal diversity

Samantha Flores-Treviño, Jessica Lizzeth Gutiérrez-Ferman, Rayo Morfín-Otero, Eduardo Rodríguez-Noriega, Diego Estrada-Rivadeneyra, Catalina Rivas-Morales, Jorge M Llaca-Díaz, Adrián Camacho-Ortíz, Soraya Mendoza-Olazarán, Elvira Garza-González

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Stenotrophomonas maltophilia is an important multidrug-resistant nosocomial pathogen associated with high mortality. Our aim was to examine antimicrobial susceptibility, biofilm production and clonal relatedness of clinical isolates of S. maltophilia. S. maltophilia isolates were collected between 2006 and 2013 from two tertiary care hospitals in Mexico. Antimicrobial susceptibility was evaluated by the broth microdilution method. PCR was used to determine the presence of β-lactamase genes L1 and L2. Biofilm formation was assessed with crystal violet staining. Clonal relatedness was determined by PFGE. Among the 119 collected S. maltophilia isolates, 73 (61.3%) were from the respiratory tract. Resistance levels exceeded 75% for imipenem, meropenem, ampicillin, aztreonam, gentamicin and tobramycin. Resistance to trimethoprim-sulfamethoxazole was 32.8%. L1 and L2 genes were detected in 77.1% (91/118) and 66.9% (79/118) of isolates, respectively. All S. maltophilia strains were able to produce biofilms. Strains were classified as weak (47.9%, 57/119), moderate (38.7%, 46/119), or strong (13.4%, 16/119) biofilm producers. A total of 89 distinct PFGE types were identified and 21.6% (22/102) of the isolates were distributed in nine clusters. This is the first study in Mexico to reveal characteristics of clinical isolates of S. maltophilia. Clonal diversity data indicate low cross-transmission of S. maltophilia in a hospital setting. The high antibiotic resistance underscores the need for continuous surveillance of S. maltophilia in hospital settings in Mexico.

Original languageEnglish
Pages (from-to)1524-30
Number of pages7
JournalJournal of Medical Microbiology
Volume63
Issue numberPt 11
DOIs
Publication statusPublished - Nov 2014
Externally publishedYes

Fingerprint

Stenotrophomonas maltophilia
Biofilms
Mexico
meropenem
Gentian Violet
Aztreonam
Tobramycin
Imipenem
Sulfamethoxazole Drug Combination Trimethoprim
Tertiary Healthcare
Ampicillin
Microbial Drug Resistance
Gentamicins
Tertiary Care Centers
Respiratory System
Genes
Staining and Labeling
Polymerase Chain Reaction
Mortality

Bibliographical note

© 2014 The Authors.

Cite this

Flores-Treviño, S., Gutiérrez-Ferman, J. L., Morfín-Otero, R., Rodríguez-Noriega, E., Estrada-Rivadeneyra, D., Rivas-Morales, C., ... Garza-González, E. (2014). Stenotrophomonas maltophilia in Mexico: antimicrobial resistance, biofilm formation and clonal diversity. Journal of Medical Microbiology, 63(Pt 11), 1524-30. https://doi.org/10.1099/jmm.0.074385-0
Flores-Treviño, Samantha ; Gutiérrez-Ferman, Jessica Lizzeth ; Morfín-Otero, Rayo ; Rodríguez-Noriega, Eduardo ; Estrada-Rivadeneyra, Diego ; Rivas-Morales, Catalina ; Llaca-Díaz, Jorge M ; Camacho-Ortíz, Adrián ; Mendoza-Olazarán, Soraya ; Garza-González, Elvira. / Stenotrophomonas maltophilia in Mexico : antimicrobial resistance, biofilm formation and clonal diversity. In: Journal of Medical Microbiology. 2014 ; Vol. 63, No. Pt 11. pp. 1524-30.
@article{7dc0b6ef32494385b310a6ce38332043,
title = "Stenotrophomonas maltophilia in Mexico: antimicrobial resistance, biofilm formation and clonal diversity",
abstract = "Stenotrophomonas maltophilia is an important multidrug-resistant nosocomial pathogen associated with high mortality. Our aim was to examine antimicrobial susceptibility, biofilm production and clonal relatedness of clinical isolates of S. maltophilia. S. maltophilia isolates were collected between 2006 and 2013 from two tertiary care hospitals in Mexico. Antimicrobial susceptibility was evaluated by the broth microdilution method. PCR was used to determine the presence of β-lactamase genes L1 and L2. Biofilm formation was assessed with crystal violet staining. Clonal relatedness was determined by PFGE. Among the 119 collected S. maltophilia isolates, 73 (61.3{\%}) were from the respiratory tract. Resistance levels exceeded 75{\%} for imipenem, meropenem, ampicillin, aztreonam, gentamicin and tobramycin. Resistance to trimethoprim-sulfamethoxazole was 32.8{\%}. L1 and L2 genes were detected in 77.1{\%} (91/118) and 66.9{\%} (79/118) of isolates, respectively. All S. maltophilia strains were able to produce biofilms. Strains were classified as weak (47.9{\%}, 57/119), moderate (38.7{\%}, 46/119), or strong (13.4{\%}, 16/119) biofilm producers. A total of 89 distinct PFGE types were identified and 21.6{\%} (22/102) of the isolates were distributed in nine clusters. This is the first study in Mexico to reveal characteristics of clinical isolates of S. maltophilia. Clonal diversity data indicate low cross-transmission of S. maltophilia in a hospital setting. The high antibiotic resistance underscores the need for continuous surveillance of S. maltophilia in hospital settings in Mexico.",
author = "Samantha Flores-Trevi{\~n}o and Guti{\'e}rrez-Ferman, {Jessica Lizzeth} and Rayo Morf{\'i}n-Otero and Eduardo Rodr{\'i}guez-Noriega and Diego Estrada-Rivadeneyra and Catalina Rivas-Morales and Llaca-D{\'i}az, {Jorge M} and Adri{\'a}n Camacho-Ort{\'i}z and Soraya Mendoza-Olazar{\'a}n and Elvira Garza-Gonz{\'a}lez",
note = "{\circledC} 2014 The Authors.",
year = "2014",
month = "11",
doi = "10.1099/jmm.0.074385-0",
language = "English",
volume = "63",
pages = "1524--30",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "Pt 11",

}

Flores-Treviño, S, Gutiérrez-Ferman, JL, Morfín-Otero, R, Rodríguez-Noriega, E, Estrada-Rivadeneyra, D, Rivas-Morales, C, Llaca-Díaz, JM, Camacho-Ortíz, A, Mendoza-Olazarán, S & Garza-González, E 2014, 'Stenotrophomonas maltophilia in Mexico: antimicrobial resistance, biofilm formation and clonal diversity', Journal of Medical Microbiology, vol. 63, no. Pt 11, pp. 1524-30. https://doi.org/10.1099/jmm.0.074385-0

Stenotrophomonas maltophilia in Mexico : antimicrobial resistance, biofilm formation and clonal diversity. / Flores-Treviño, Samantha; Gutiérrez-Ferman, Jessica Lizzeth; Morfín-Otero, Rayo; Rodríguez-Noriega, Eduardo; Estrada-Rivadeneyra, Diego; Rivas-Morales, Catalina; Llaca-Díaz, Jorge M; Camacho-Ortíz, Adrián; Mendoza-Olazarán, Soraya; Garza-González, Elvira.

In: Journal of Medical Microbiology, Vol. 63, No. Pt 11, 11.2014, p. 1524-30.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stenotrophomonas maltophilia in Mexico

T2 - antimicrobial resistance, biofilm formation and clonal diversity

AU - Flores-Treviño, Samantha

AU - Gutiérrez-Ferman, Jessica Lizzeth

AU - Morfín-Otero, Rayo

AU - Rodríguez-Noriega, Eduardo

AU - Estrada-Rivadeneyra, Diego

AU - Rivas-Morales, Catalina

AU - Llaca-Díaz, Jorge M

AU - Camacho-Ortíz, Adrián

AU - Mendoza-Olazarán, Soraya

AU - Garza-González, Elvira

N1 - © 2014 The Authors.

PY - 2014/11

Y1 - 2014/11

N2 - Stenotrophomonas maltophilia is an important multidrug-resistant nosocomial pathogen associated with high mortality. Our aim was to examine antimicrobial susceptibility, biofilm production and clonal relatedness of clinical isolates of S. maltophilia. S. maltophilia isolates were collected between 2006 and 2013 from two tertiary care hospitals in Mexico. Antimicrobial susceptibility was evaluated by the broth microdilution method. PCR was used to determine the presence of β-lactamase genes L1 and L2. Biofilm formation was assessed with crystal violet staining. Clonal relatedness was determined by PFGE. Among the 119 collected S. maltophilia isolates, 73 (61.3%) were from the respiratory tract. Resistance levels exceeded 75% for imipenem, meropenem, ampicillin, aztreonam, gentamicin and tobramycin. Resistance to trimethoprim-sulfamethoxazole was 32.8%. L1 and L2 genes were detected in 77.1% (91/118) and 66.9% (79/118) of isolates, respectively. All S. maltophilia strains were able to produce biofilms. Strains were classified as weak (47.9%, 57/119), moderate (38.7%, 46/119), or strong (13.4%, 16/119) biofilm producers. A total of 89 distinct PFGE types were identified and 21.6% (22/102) of the isolates were distributed in nine clusters. This is the first study in Mexico to reveal characteristics of clinical isolates of S. maltophilia. Clonal diversity data indicate low cross-transmission of S. maltophilia in a hospital setting. The high antibiotic resistance underscores the need for continuous surveillance of S. maltophilia in hospital settings in Mexico.

AB - Stenotrophomonas maltophilia is an important multidrug-resistant nosocomial pathogen associated with high mortality. Our aim was to examine antimicrobial susceptibility, biofilm production and clonal relatedness of clinical isolates of S. maltophilia. S. maltophilia isolates were collected between 2006 and 2013 from two tertiary care hospitals in Mexico. Antimicrobial susceptibility was evaluated by the broth microdilution method. PCR was used to determine the presence of β-lactamase genes L1 and L2. Biofilm formation was assessed with crystal violet staining. Clonal relatedness was determined by PFGE. Among the 119 collected S. maltophilia isolates, 73 (61.3%) were from the respiratory tract. Resistance levels exceeded 75% for imipenem, meropenem, ampicillin, aztreonam, gentamicin and tobramycin. Resistance to trimethoprim-sulfamethoxazole was 32.8%. L1 and L2 genes were detected in 77.1% (91/118) and 66.9% (79/118) of isolates, respectively. All S. maltophilia strains were able to produce biofilms. Strains were classified as weak (47.9%, 57/119), moderate (38.7%, 46/119), or strong (13.4%, 16/119) biofilm producers. A total of 89 distinct PFGE types were identified and 21.6% (22/102) of the isolates were distributed in nine clusters. This is the first study in Mexico to reveal characteristics of clinical isolates of S. maltophilia. Clonal diversity data indicate low cross-transmission of S. maltophilia in a hospital setting. The high antibiotic resistance underscores the need for continuous surveillance of S. maltophilia in hospital settings in Mexico.

U2 - 10.1099/jmm.0.074385-0

DO - 10.1099/jmm.0.074385-0

M3 - Article

C2 - 25165124

VL - 63

SP - 1524

EP - 1530

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - Pt 11

ER -

Flores-Treviño S, Gutiérrez-Ferman JL, Morfín-Otero R, Rodríguez-Noriega E, Estrada-Rivadeneyra D, Rivas-Morales C et al. Stenotrophomonas maltophilia in Mexico: antimicrobial resistance, biofilm formation and clonal diversity. Journal of Medical Microbiology. 2014 Nov;63(Pt 11):1524-30. https://doi.org/10.1099/jmm.0.074385-0