Spironolactone Effect in Hepatic Ischemia/Reperfusion Injury in Wistar Rats

Julio César Jiménez Pérez, Araní Casillas Ramírez, Liliana Torres González, Linda Elsa Muñoz Espinosa, Marlene Marisol Perales Quintana, Gabriela Alarcón Galván, Homero Zapata Chavira, Francisco Javier Guzmán De La Garza, Carlos Rodrigo Cámara Lemarroy, Nancy Esthela Fernández Garza, Edelmiro Pérez Rodríguez, Paula Cordero Pérez

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

© 2016 Julio César Jiménez Pérez et al. Introduction. Ischemia/reperfusion (IR) injury, often associated with liver surgery, is an unresolved problem in the clinical practice. Spironolactone is an antagonist of aldosterone that has shown benefits over IR injury in several tissues, but its effects in hepatic IR are unknown. Objective. To evaluate the effect of spironolactone on IR-induced damage in liver. Materials and Methods. Total hepatic ischemia was induced in rats for 20 min followed by 60 min of reperfusion. Spironolactone was administered and hepatic injury, cytokine production, and oxidative stress were assessed. Results. After IR, increased transaminases levels and widespread acute inflammatory infiltrate, disorganization of hepatic hemorrhage trabeculae, and presence of apoptotic bodies were observed. Administration of SPI reduced biochemical and histological parameters of liver injury. SPI treatment increased IL-6 levels when compared with IR group but did not modify either IL-1β or TNF-α with respect to IR group. Regarding oxidative stress, increased levels of catalase activity were recorded in IR + SPI group in comparison with group without treatment, whereas MDA levels were similar in IR + SPI and IR groups. Conclusions. Spironolactone reduced the liver damage induced by IR, and this was associated with an increase in IL-6 production and catalase activity.
Original languageEnglish
JournalOxidative Medicine and Cellular Longevity
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Fingerprint

Spironolactone
Reperfusion Injury
Liver
Wistar Rats
Rats
Reperfusion
Ischemia
Oxidative stress
Catalase
Interleukin-6
Mineralocorticoid Receptor Antagonists
Transaminases
Interleukin-1
Surgery
Tissue
Cytokines
Oxidative Stress
Wounds and Injuries
Hemorrhage

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ageing
  • Cell Biology

Cite this

Jiménez Pérez, J. C., Casillas Ramírez, A., Torres González, L., Muñoz Espinosa, L. E., Perales Quintana, M. M., Alarcón Galván, G., ... Cordero Pérez, P. (2016). Spironolactone Effect in Hepatic Ischemia/Reperfusion Injury in Wistar Rats. Oxidative Medicine and Cellular Longevity. https://doi.org/10.1155/2016/3196431
Jiménez Pérez, Julio César ; Casillas Ramírez, Araní ; Torres González, Liliana ; Muñoz Espinosa, Linda Elsa ; Perales Quintana, Marlene Marisol ; Alarcón Galván, Gabriela ; Zapata Chavira, Homero ; Guzmán De La Garza, Francisco Javier ; Cámara Lemarroy, Carlos Rodrigo ; Fernández Garza, Nancy Esthela ; Pérez Rodríguez, Edelmiro ; Cordero Pérez, Paula. / Spironolactone Effect in Hepatic Ischemia/Reperfusion Injury in Wistar Rats. In: Oxidative Medicine and Cellular Longevity. 2016.
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Jiménez Pérez, JC, Casillas Ramírez, A, Torres González, L, Muñoz Espinosa, LE, Perales Quintana, MM, Alarcón Galván, G, Zapata Chavira, H, Guzmán De La Garza, FJ, Cámara Lemarroy, CR, Fernández Garza, NE, Pérez Rodríguez, E & Cordero Pérez, P 2016, 'Spironolactone Effect in Hepatic Ischemia/Reperfusion Injury in Wistar Rats', Oxidative Medicine and Cellular Longevity. https://doi.org/10.1155/2016/3196431

Spironolactone Effect in Hepatic Ischemia/Reperfusion Injury in Wistar Rats. / Jiménez Pérez, Julio César; Casillas Ramírez, Araní; Torres González, Liliana; Muñoz Espinosa, Linda Elsa; Perales Quintana, Marlene Marisol; Alarcón Galván, Gabriela; Zapata Chavira, Homero; Guzmán De La Garza, Francisco Javier; Cámara Lemarroy, Carlos Rodrigo; Fernández Garza, Nancy Esthela; Pérez Rodríguez, Edelmiro; Cordero Pérez, Paula.

In: Oxidative Medicine and Cellular Longevity, 01.01.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Spironolactone Effect in Hepatic Ischemia/Reperfusion Injury in Wistar Rats

AU - Jiménez Pérez, Julio César

AU - Casillas Ramírez, Araní

AU - Torres González, Liliana

AU - Muñoz Espinosa, Linda Elsa

AU - Perales Quintana, Marlene Marisol

AU - Alarcón Galván, Gabriela

AU - Zapata Chavira, Homero

AU - Guzmán De La Garza, Francisco Javier

AU - Cámara Lemarroy, Carlos Rodrigo

AU - Fernández Garza, Nancy Esthela

AU - Pérez Rodríguez, Edelmiro

AU - Cordero Pérez, Paula

PY - 2016/1/1

Y1 - 2016/1/1

N2 - © 2016 Julio César Jiménez Pérez et al. Introduction. Ischemia/reperfusion (IR) injury, often associated with liver surgery, is an unresolved problem in the clinical practice. Spironolactone is an antagonist of aldosterone that has shown benefits over IR injury in several tissues, but its effects in hepatic IR are unknown. Objective. To evaluate the effect of spironolactone on IR-induced damage in liver. Materials and Methods. Total hepatic ischemia was induced in rats for 20 min followed by 60 min of reperfusion. Spironolactone was administered and hepatic injury, cytokine production, and oxidative stress were assessed. Results. After IR, increased transaminases levels and widespread acute inflammatory infiltrate, disorganization of hepatic hemorrhage trabeculae, and presence of apoptotic bodies were observed. Administration of SPI reduced biochemical and histological parameters of liver injury. SPI treatment increased IL-6 levels when compared with IR group but did not modify either IL-1β or TNF-α with respect to IR group. Regarding oxidative stress, increased levels of catalase activity were recorded in IR + SPI group in comparison with group without treatment, whereas MDA levels were similar in IR + SPI and IR groups. Conclusions. Spironolactone reduced the liver damage induced by IR, and this was associated with an increase in IL-6 production and catalase activity.

AB - © 2016 Julio César Jiménez Pérez et al. Introduction. Ischemia/reperfusion (IR) injury, often associated with liver surgery, is an unresolved problem in the clinical practice. Spironolactone is an antagonist of aldosterone that has shown benefits over IR injury in several tissues, but its effects in hepatic IR are unknown. Objective. To evaluate the effect of spironolactone on IR-induced damage in liver. Materials and Methods. Total hepatic ischemia was induced in rats for 20 min followed by 60 min of reperfusion. Spironolactone was administered and hepatic injury, cytokine production, and oxidative stress were assessed. Results. After IR, increased transaminases levels and widespread acute inflammatory infiltrate, disorganization of hepatic hemorrhage trabeculae, and presence of apoptotic bodies were observed. Administration of SPI reduced biochemical and histological parameters of liver injury. SPI treatment increased IL-6 levels when compared with IR group but did not modify either IL-1β or TNF-α with respect to IR group. Regarding oxidative stress, increased levels of catalase activity were recorded in IR + SPI group in comparison with group without treatment, whereas MDA levels were similar in IR + SPI and IR groups. Conclusions. Spironolactone reduced the liver damage induced by IR, and this was associated with an increase in IL-6 production and catalase activity.

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DO - 10.1155/2016/3196431

M3 - Article

JO - Oxidative Medicine and Cellular Longevity

JF - Oxidative Medicine and Cellular Longevity

SN - 1942-0900

ER -

Jiménez Pérez JC, Casillas Ramírez A, Torres González L, Muñoz Espinosa LE, Perales Quintana MM, Alarcón Galván G et al. Spironolactone Effect in Hepatic Ischemia/Reperfusion Injury in Wistar Rats. Oxidative Medicine and Cellular Longevity. 2016 Jan 1. https://doi.org/10.1155/2016/3196431