Selenite restores Pax6 expression in neuronal cells of chronically arsenic-exposed Golden Syrian hamsters

Alain Aguirre-Vázquez, Adriana Sampayo-Reyes, Laura González-Escalante, Alba Hernández, Ricard Marcos, Fabiola Castorena-Torres, Gerardo Lozano-Garza, Reyes Taméz-Guerra, Mario Bermúdez de León

Research output: Contribution to journalArticle

Abstract

Arsenic is a worldwide environmental pollutant that generates public health concerns. Various types of cancers and other diseases, including neurological disorders, have been associated with human consumption of arsenic in drinking water. At the molecular level, arsenic and its metabolites have the capacity to provoke genome instability, causing altered expression of genes. One such target of arsenic is the Pax6 gene that encodes a transcription factor in neuronal cells. The aim of this study was to evaluate the effect of two antioxidants, a-tocopheryl succinate (α-TOS) and sodium selenite, on Pax6 gene expression levels in the forebrain and cerebellum of Golden Syrian hamsters chronically exposed to arsenic in drinking water. Animals were divided into six groups. Using quantitative real-time reverse transcriptase (RT)- PCR analysis, we confirmed that arsenic downregulates Pax6 expression in nervous tissues by 53 ± 21% and 32 ± 7% in the forebrain and cerebellum, respectively. In the presence of arsenic, treatment with a-TOS did not modify Pax6 expression in nervous tissues; however, sodium selenite completely restored Pax6 expression in the arsenic-exposed hamster forebrain, but not the cerebellum. Although our results suggest the use of selenite to restore the expression of a neuronal gene in arsenic-exposed animals, its use and efficacy in the human population require further studies.
Original languageEnglish
Pages (from-to)635-639
Number of pages5
JournalActa Biochimica Polonica
DOIs
Publication statusPublished - 1 Jan 2017

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Selenious Acid
Mesocricetus
Arsenic
Prosencephalon
Genes
Cerebellum
Sodium Selenite
Nerve Tissue
Drinking Water
Animals
Tissue
Gene Expression
Environmental Pollutants
Genomic Instability
RNA-Directed DNA Polymerase
Succinic Acid
Public health
Metabolites
Nervous System Diseases
Reverse Transcriptase Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Aguirre-Vázquez, A., Sampayo-Reyes, A., González-Escalante, L., Hernández, A., Marcos, R., Castorena-Torres, F., ... de León, M. B. (2017). Selenite restores Pax6 expression in neuronal cells of chronically arsenic-exposed Golden Syrian hamsters. Acta Biochimica Polonica, 635-639. https://doi.org/10.18388/abp.2017_1607
Aguirre-Vázquez, Alain ; Sampayo-Reyes, Adriana ; González-Escalante, Laura ; Hernández, Alba ; Marcos, Ricard ; Castorena-Torres, Fabiola ; Lozano-Garza, Gerardo ; Taméz-Guerra, Reyes ; de León, Mario Bermúdez. / Selenite restores Pax6 expression in neuronal cells of chronically arsenic-exposed Golden Syrian hamsters. In: Acta Biochimica Polonica. 2017 ; pp. 635-639.
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Aguirre-Vázquez, A, Sampayo-Reyes, A, González-Escalante, L, Hernández, A, Marcos, R, Castorena-Torres, F, Lozano-Garza, G, Taméz-Guerra, R & de León, MB 2017, 'Selenite restores Pax6 expression in neuronal cells of chronically arsenic-exposed Golden Syrian hamsters', Acta Biochimica Polonica, pp. 635-639. https://doi.org/10.18388/abp.2017_1607

Selenite restores Pax6 expression in neuronal cells of chronically arsenic-exposed Golden Syrian hamsters. / Aguirre-Vázquez, Alain; Sampayo-Reyes, Adriana; González-Escalante, Laura; Hernández, Alba; Marcos, Ricard; Castorena-Torres, Fabiola; Lozano-Garza, Gerardo; Taméz-Guerra, Reyes; de León, Mario Bermúdez.

In: Acta Biochimica Polonica, 01.01.2017, p. 635-639.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Selenite restores Pax6 expression in neuronal cells of chronically arsenic-exposed Golden Syrian hamsters

AU - Aguirre-Vázquez, Alain

AU - Sampayo-Reyes, Adriana

AU - González-Escalante, Laura

AU - Hernández, Alba

AU - Marcos, Ricard

AU - Castorena-Torres, Fabiola

AU - Lozano-Garza, Gerardo

AU - Taméz-Guerra, Reyes

AU - de León, Mario Bermúdez

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Arsenic is a worldwide environmental pollutant that generates public health concerns. Various types of cancers and other diseases, including neurological disorders, have been associated with human consumption of arsenic in drinking water. At the molecular level, arsenic and its metabolites have the capacity to provoke genome instability, causing altered expression of genes. One such target of arsenic is the Pax6 gene that encodes a transcription factor in neuronal cells. The aim of this study was to evaluate the effect of two antioxidants, a-tocopheryl succinate (α-TOS) and sodium selenite, on Pax6 gene expression levels in the forebrain and cerebellum of Golden Syrian hamsters chronically exposed to arsenic in drinking water. Animals were divided into six groups. Using quantitative real-time reverse transcriptase (RT)- PCR analysis, we confirmed that arsenic downregulates Pax6 expression in nervous tissues by 53 ± 21% and 32 ± 7% in the forebrain and cerebellum, respectively. In the presence of arsenic, treatment with a-TOS did not modify Pax6 expression in nervous tissues; however, sodium selenite completely restored Pax6 expression in the arsenic-exposed hamster forebrain, but not the cerebellum. Although our results suggest the use of selenite to restore the expression of a neuronal gene in arsenic-exposed animals, its use and efficacy in the human population require further studies.

AB - Arsenic is a worldwide environmental pollutant that generates public health concerns. Various types of cancers and other diseases, including neurological disorders, have been associated with human consumption of arsenic in drinking water. At the molecular level, arsenic and its metabolites have the capacity to provoke genome instability, causing altered expression of genes. One such target of arsenic is the Pax6 gene that encodes a transcription factor in neuronal cells. The aim of this study was to evaluate the effect of two antioxidants, a-tocopheryl succinate (α-TOS) and sodium selenite, on Pax6 gene expression levels in the forebrain and cerebellum of Golden Syrian hamsters chronically exposed to arsenic in drinking water. Animals were divided into six groups. Using quantitative real-time reverse transcriptase (RT)- PCR analysis, we confirmed that arsenic downregulates Pax6 expression in nervous tissues by 53 ± 21% and 32 ± 7% in the forebrain and cerebellum, respectively. In the presence of arsenic, treatment with a-TOS did not modify Pax6 expression in nervous tissues; however, sodium selenite completely restored Pax6 expression in the arsenic-exposed hamster forebrain, but not the cerebellum. Although our results suggest the use of selenite to restore the expression of a neuronal gene in arsenic-exposed animals, its use and efficacy in the human population require further studies.

U2 - 10.18388/abp.2017_1607

DO - 10.18388/abp.2017_1607

M3 - Article

SP - 635

EP - 639

JO - Acta Biochimica Polonica

JF - Acta Biochimica Polonica

SN - 0001-527X

ER -

Aguirre-Vázquez A, Sampayo-Reyes A, González-Escalante L, Hernández A, Marcos R, Castorena-Torres F et al. Selenite restores Pax6 expression in neuronal cells of chronically arsenic-exposed Golden Syrian hamsters. Acta Biochimica Polonica. 2017 Jan 1;635-639. https://doi.org/10.18388/abp.2017_1607