Reproducibility of non-contact specular microscopy with regards to the number of evaluated cells

Maria Elisa Pizarro-Barrera, Manuel A. Garza-León, F. B D De La Vega, Ramón Naranjo-Tackman

Research output: Contribution to journalArticle

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Abstract

Objective: To evaluate the corneal endothelium density according to the number of evaluated cells and to find the most constant number of cells. Methods: Prospective transverse and observational study. Non-contact specular microscopies were realized, counting 20, 30, 40, 50 and 70 cells. The data were analyzed to find the number of cells closer to the average and the variability of the results. Results: Thirty specular microscopies were evaluated, from healthy volunteers. 66% male, with an average age of 28.3 ± 13.72 (range of 17-84). The average of endothelial cells in the group of 20 cells was 2541.66 ± 451.64 cells/mm2, in the 30 cells group was 2552.70 ± 431.12, in the 40 cells group was 2439.53 ± 417.36, in the 50 cells group was 2542.16 ± 97.46 and in the 70 cells group it was 2569.60 ± 361.72. The general average was 2549.26 ± 407.29. There was no significant statistical difference between the groups. Conclusions: In this group of healthy patients, there's a high reproducibility in all endothelial cell counts and there's no difference between the number of cells studied.
Original languageEnglish
Pages (from-to)148-151
Number of pages4
JournalRevista Mexicana de Oftalmologia
Publication statusPublished - 1 May 2007
Externally publishedYes

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Microscopy
Cell Count
Endothelial Cells
Corneal Endothelium
Observational Studies
Healthy Volunteers
Prospective Studies

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Pizarro-Barrera, Maria Elisa ; Garza-León, Manuel A. ; De La Vega, F. B D ; Naranjo-Tackman, Ramón. / Reproducibility of non-contact specular microscopy with regards to the number of evaluated cells. In: Revista Mexicana de Oftalmologia. 2007 ; pp. 148-151.
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abstract = "Objective: To evaluate the corneal endothelium density according to the number of evaluated cells and to find the most constant number of cells. Methods: Prospective transverse and observational study. Non-contact specular microscopies were realized, counting 20, 30, 40, 50 and 70 cells. The data were analyzed to find the number of cells closer to the average and the variability of the results. Results: Thirty specular microscopies were evaluated, from healthy volunteers. 66{\%} male, with an average age of 28.3 ± 13.72 (range of 17-84). The average of endothelial cells in the group of 20 cells was 2541.66 ± 451.64 cells/mm2, in the 30 cells group was 2552.70 ± 431.12, in the 40 cells group was 2439.53 ± 417.36, in the 50 cells group was 2542.16 ± 97.46 and in the 70 cells group it was 2569.60 ± 361.72. The general average was 2549.26 ± 407.29. There was no significant statistical difference between the groups. Conclusions: In this group of healthy patients, there's a high reproducibility in all endothelial cell counts and there's no difference between the number of cells studied.",
author = "Pizarro-Barrera, {Maria Elisa} and Garza-Le{\'o}n, {Manuel A.} and {De La Vega}, {F. B D} and Ram{\'o}n Naranjo-Tackman",
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Reproducibility of non-contact specular microscopy with regards to the number of evaluated cells. / Pizarro-Barrera, Maria Elisa; Garza-León, Manuel A.; De La Vega, F. B D; Naranjo-Tackman, Ramón.

In: Revista Mexicana de Oftalmologia, 01.05.2007, p. 148-151.

Research output: Contribution to journalArticle

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T1 - Reproducibility of non-contact specular microscopy with regards to the number of evaluated cells

AU - Pizarro-Barrera, Maria Elisa

AU - Garza-León, Manuel A.

AU - De La Vega, F. B D

AU - Naranjo-Tackman, Ramón

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Objective: To evaluate the corneal endothelium density according to the number of evaluated cells and to find the most constant number of cells. Methods: Prospective transverse and observational study. Non-contact specular microscopies were realized, counting 20, 30, 40, 50 and 70 cells. The data were analyzed to find the number of cells closer to the average and the variability of the results. Results: Thirty specular microscopies were evaluated, from healthy volunteers. 66% male, with an average age of 28.3 ± 13.72 (range of 17-84). The average of endothelial cells in the group of 20 cells was 2541.66 ± 451.64 cells/mm2, in the 30 cells group was 2552.70 ± 431.12, in the 40 cells group was 2439.53 ± 417.36, in the 50 cells group was 2542.16 ± 97.46 and in the 70 cells group it was 2569.60 ± 361.72. The general average was 2549.26 ± 407.29. There was no significant statistical difference between the groups. Conclusions: In this group of healthy patients, there's a high reproducibility in all endothelial cell counts and there's no difference between the number of cells studied.

AB - Objective: To evaluate the corneal endothelium density according to the number of evaluated cells and to find the most constant number of cells. Methods: Prospective transverse and observational study. Non-contact specular microscopies were realized, counting 20, 30, 40, 50 and 70 cells. The data were analyzed to find the number of cells closer to the average and the variability of the results. Results: Thirty specular microscopies were evaluated, from healthy volunteers. 66% male, with an average age of 28.3 ± 13.72 (range of 17-84). The average of endothelial cells in the group of 20 cells was 2541.66 ± 451.64 cells/mm2, in the 30 cells group was 2552.70 ± 431.12, in the 40 cells group was 2439.53 ± 417.36, in the 50 cells group was 2542.16 ± 97.46 and in the 70 cells group it was 2569.60 ± 361.72. The general average was 2549.26 ± 407.29. There was no significant statistical difference between the groups. Conclusions: In this group of healthy patients, there's a high reproducibility in all endothelial cell counts and there's no difference between the number of cells studied.

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