Priming of Hypothalamic Ghrelin Signaling and Microglia Activation Exacerbate Feeding in Rats' Offspring Following Maternal Overnutrition

Roger Maldonado-Ruiz, Marcela Cardenas-Tueme, Larisa Montalvo-Martinez, Roman Vidaltamayo, Lourdes Garza-Ocanas, Diana Resendez-Perez, Alberto Camacho

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5 Citations (Scopus)

Abstract

Maternal overnutrition during pregnancy leads to metabolic alterations, including obesity, hyperphagia, and inflammation in the offspring. Nutritional priming of central inflammation and its role in ghrelin sensitivity during fed and fasted states have not been analyzed. The current study aims to identify the effect of maternal programming on microglia activation and ghrelin-induced activation of hypothalamic neurons leading to food intake response. We employed a nutritional programming model exposing female Wistar rats to a cafeteria diet (CAF) from pre-pregnancy to weaning. Food intake in male offspring was determined daily after fasting and subcutaneous injection of ghrelin. Hypothalamic ghrelin sensitivity and microglia activation was evaluated using immunodetection for Iba-1 and c-Fos markers, and Western blot for TBK1 signaling. Release of TNF-alpha, IL-6, and IL-1β after stimulation with palmitic, oleic, linoleic acid, or C6 ceramide in primary microglia culture were quantified using ELISA. We found that programmed offspring by CAF diet exhibits overfeeding after fasting and peripheral ghrelin administration, which correlates with an increase in the hypothalamic Iba-1 microglia marker and c-Fos cell activation. Additionally, in contrast to oleic, linoleic, or C6 ceramide stimulation in primary microglia culture, stimulation with palmitic acid for 24 h promotes TNF-alpha, IL-6, and IL-1β release and TBK1 activation. Notably, intracerebroventricular (i.c.v.) palmitic acid or LPS inoculation for five days promotes daily increase in food intake and food consumption after ghrelin administration. Finally, we found that i.c.v. palmitic acid substantially activates hypothalamic Iba-1 microglia marker and c-Fos. Together, our results suggest that maternal nutritional programing primes ghrelin sensitivity and microglia activation, which potentially might mirror hypothalamic administration of the saturated palmitic acid.

Original languageEnglish
Article number1241
JournalNutrients
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 2019

Bibliographical note

Funding Information:
Funding: This work was funded by National Council of Science and Technology in Mexico (CONACYT) (CB-2015-255317 to Alberto Camacho and CB-2013-220342 to Roman Vidaltamayo), 582196 CONACYT for Larisa Montalvo-Martínez, 573686 CONACYT for Roger Maldonado-Ruiz, 650620 CONACYT for Marcela Cárdenas-Tueme and División de Extensión, Consultoría e Investigación, Universidad de Monterrey grants (Plan de Investigación 2018 and 2019) to R. Vidaltamayo.

Publisher Copyright:
© 2019, MDPI AG. All rights reserved.

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

  • Food Science
  • Nutrition and Dietetics

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