Phenotypic and molecular identification of Fonsecaea pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela

O. Carolina Rojas, Rafael B.R. León-Cachón, Antonio Alí Pérez-Maya, Marcelino Aguirre-Garza, María G. Moreno-Treviño, Gloria M. González

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chromoblastomycosis is a chronic granulomatous disease caused frequently by fungi of the Fonsecaea genus. The objective of this study was the phenotypic and molecular identification of F. pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela. Ten strains were included in this study. For phenotypic identification, we used macroscopic and microscopic morphologies, carbohydrate assimilation test, urea hydrolysis, cixcloheximide tolerance, proteolitic activity and the thermotolerance test. The antifungal activity of five drugs was evaluated against the isolates. Molecular identification was performed by sequencing the internal transcribed spacer (ITS) ribosomal DNA regions of the isolated strains. The physiological analysis and morphological features were variable and the precise identification was not possible. All isolates were susceptible to itraconazole, terbinafine, voriconazole and posaconazole. Amphotericin B was the least effective drug. The alignment of the 559-nucleotide ITS sequences from our strains compared with sequences of GenBank revealed high homology with F. pedrosoi (EU285266.1). In this study, all patients were from rural areas, six from Mexico and four from Venezuela. Ten isolates were identified by phenotypic and molecular analysis, using ITS sequence and demonstrated that nine isolates from Mexico and Venezuela were 100% homologous and one isolate showed a small genetic distance.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalMycoses
Volume58
Issue number5
DOIs
Publication statusPublished - 1 Jan 2015

Fingerprint

Chromoblastomycosis
Venezuela
Mexico
terbinafine
Ribosomal Spacer DNA
Chronic Granulomatous Disease
Itraconazole
Nucleic Acid Databases
Amphotericin B
Pharmaceutical Preparations
Urea
Hydrolysis
Fungi
Nucleotides
Carbohydrates

All Science Journal Classification (ASJC) codes

  • Dermatology
  • Infectious Diseases

Cite this

@article{0abdd85ccb2f404196bfc63387710b4b,
title = "Phenotypic and molecular identification of Fonsecaea pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela",
abstract = "Chromoblastomycosis is a chronic granulomatous disease caused frequently by fungi of the Fonsecaea genus. The objective of this study was the phenotypic and molecular identification of F. pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela. Ten strains were included in this study. For phenotypic identification, we used macroscopic and microscopic morphologies, carbohydrate assimilation test, urea hydrolysis, cixcloheximide tolerance, proteolitic activity and the thermotolerance test. The antifungal activity of five drugs was evaluated against the isolates. Molecular identification was performed by sequencing the internal transcribed spacer (ITS) ribosomal DNA regions of the isolated strains. The physiological analysis and morphological features were variable and the precise identification was not possible. All isolates were susceptible to itraconazole, terbinafine, voriconazole and posaconazole. Amphotericin B was the least effective drug. The alignment of the 559-nucleotide ITS sequences from our strains compared with sequences of GenBank revealed high homology with F. pedrosoi (EU285266.1). In this study, all patients were from rural areas, six from Mexico and four from Venezuela. Ten isolates were identified by phenotypic and molecular analysis, using ITS sequence and demonstrated that nine isolates from Mexico and Venezuela were 100{\%} homologous and one isolate showed a small genetic distance.",
author = "{Carolina Rojas}, O. and Le{\'o}n-Cach{\'o}n, {Rafael B.R.} and P{\'e}rez-Maya, {Antonio Al{\'i}} and Marcelino Aguirre-Garza and Moreno-Trevi{\~n}o, {Mar{\'i}a G.} and Gonz{\'a}lez, {Gloria M.}",
year = "2015",
month = "1",
day = "1",
doi = "10.1111/myc.12308",
language = "English",
volume = "58",
pages = "267--272",
journal = "Mycoses",
issn = "0933-7407",
publisher = "Wiley-Blackwell",
number = "5",

}

Phenotypic and molecular identification of Fonsecaea pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela. / Carolina Rojas, O.; León-Cachón, Rafael B.R.; Pérez-Maya, Antonio Alí; Aguirre-Garza, Marcelino; Moreno-Treviño, María G.; González, Gloria M.

In: Mycoses, Vol. 58, No. 5, 01.01.2015, p. 267-272.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Phenotypic and molecular identification of Fonsecaea pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela

AU - Carolina Rojas, O.

AU - León-Cachón, Rafael B.R.

AU - Pérez-Maya, Antonio Alí

AU - Aguirre-Garza, Marcelino

AU - Moreno-Treviño, María G.

AU - González, Gloria M.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Chromoblastomycosis is a chronic granulomatous disease caused frequently by fungi of the Fonsecaea genus. The objective of this study was the phenotypic and molecular identification of F. pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela. Ten strains were included in this study. For phenotypic identification, we used macroscopic and microscopic morphologies, carbohydrate assimilation test, urea hydrolysis, cixcloheximide tolerance, proteolitic activity and the thermotolerance test. The antifungal activity of five drugs was evaluated against the isolates. Molecular identification was performed by sequencing the internal transcribed spacer (ITS) ribosomal DNA regions of the isolated strains. The physiological analysis and morphological features were variable and the precise identification was not possible. All isolates were susceptible to itraconazole, terbinafine, voriconazole and posaconazole. Amphotericin B was the least effective drug. The alignment of the 559-nucleotide ITS sequences from our strains compared with sequences of GenBank revealed high homology with F. pedrosoi (EU285266.1). In this study, all patients were from rural areas, six from Mexico and four from Venezuela. Ten isolates were identified by phenotypic and molecular analysis, using ITS sequence and demonstrated that nine isolates from Mexico and Venezuela were 100% homologous and one isolate showed a small genetic distance.

AB - Chromoblastomycosis is a chronic granulomatous disease caused frequently by fungi of the Fonsecaea genus. The objective of this study was the phenotypic and molecular identification of F. pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela. Ten strains were included in this study. For phenotypic identification, we used macroscopic and microscopic morphologies, carbohydrate assimilation test, urea hydrolysis, cixcloheximide tolerance, proteolitic activity and the thermotolerance test. The antifungal activity of five drugs was evaluated against the isolates. Molecular identification was performed by sequencing the internal transcribed spacer (ITS) ribosomal DNA regions of the isolated strains. The physiological analysis and morphological features were variable and the precise identification was not possible. All isolates were susceptible to itraconazole, terbinafine, voriconazole and posaconazole. Amphotericin B was the least effective drug. The alignment of the 559-nucleotide ITS sequences from our strains compared with sequences of GenBank revealed high homology with F. pedrosoi (EU285266.1). In this study, all patients were from rural areas, six from Mexico and four from Venezuela. Ten isolates were identified by phenotypic and molecular analysis, using ITS sequence and demonstrated that nine isolates from Mexico and Venezuela were 100% homologous and one isolate showed a small genetic distance.

UR - http://www.scopus.com/inward/record.url?scp=84937633897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937633897&partnerID=8YFLogxK

U2 - 10.1111/myc.12308

DO - 10.1111/myc.12308

M3 - Article

C2 - 25728464

AN - SCOPUS:84937633897

VL - 58

SP - 267

EP - 272

JO - Mycoses

JF - Mycoses

SN - 0933-7407

IS - 5

ER -