Oxidative Stress-Dependent Coronary Endothelial Dysfunction in Obese Mice

Ana María Gamez-Mendez, Hilda Vargas-Robles, Amelia Ríos, Bruno Escalante

Research output: Contribution to journalArticlepeer-review

Abstract

Obesity is involved in several cardiovascular diseases including coronary artery disease and endothelial dysfunction. Endothelial Endothelium vasodilator and vasoconstrictor agonists play a key role in regulation of vascular tone. In this study, we evaluated coronary vascular response in an 8 weeks diet-induced obese C57BL/6 mice model. Coronary perfusion pressure in response to acetylcholine in isolated hearts from obese mice showed increased vasoconstriction and reduced vasodilation responses compared with control mice. Vascular nitric oxide assessed in situ with DAF-2 DA showed diminished levels in coronary arteries from obese mice in both basal and acetylcholine-stimulated conditions. Also, released prostacyclin was decreased in heart perfusates from obese mice, along with plasma tetrahydrobiopterin level and endothelium nitric oxide synthase dimer/monomer ratio. Obesity increased thromboxane A2 synthesis and oxidative stress evaluated by superoxide and peroxynitrite levels, compared with control mice. Obese mice treated with apocynin, a NADPH oxidase inhibitor, reversed all parameters to normal levels. These results suggest that after 8 weeks on a high-fat diet, the increase in oxidative stress lead to imbalance in vasoactive substances and consequently to endothelial dysfunction in coronary arteries.

Original languageEnglish
Article numbere0138609
Pages (from-to)e0138609
JournalPLoS One
Volume10
Issue number9
DOIs
Publication statusPublished - 18 Sep 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 Gamez-Mendez et al.

Fingerprint

Dive into the research topics of 'Oxidative Stress-Dependent Coronary Endothelial Dysfunction in Obese Mice'. Together they form a unique fingerprint.

Cite this