Occupational toluene exposure induces cytochrome P450 2E1 mRNA expression in peripheral lymphocytes

Ania Mendoza-Cantú, Fabiola Castorena-Torres, Mario Bermúdez de león, Bulmaro Cisneros, Lizbeth López-Carrillo, Aurora E. Rojas-García, Alberto Aguilar-Salinas, Maurizio Manno, Arnulfo Albores

Research output: Contribution to journalArticle

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Abstract

Print workers are exposed to organic solvents, of which the systemic toxicant toluene is a main component. Toluene induces expression of cytochrome P450 2E1 (CYP2E1), an enzyme involved in its own metabolism and that of other protoxicants, including some procarcinogens. Therefore, we investigated the association between toluene exposure and the CYP2E1 response, as assessed by mRNA content in peripheral lymphocytes or the 6-hydroxychlorzoxazone (6OH-CHZ)/chlorzoxazone (CHZ) quotient (known as CHZ metabolic ratio) in plasma, and the role of genotype (5′-flanking region RsaI/PstI polymorphic sites) in 97 male print workers. The geometric mean (GM) of toluene concentration in the air was 52.80 ppm (10-760 ppm); 54% of the study participants were exposed to toluene concentrations that exceeded the maximum permissible exposure level (MPEL). The GM of urinary hippuric acid at the end of a work shift (0.041 g/g creatinine) was elevated relative to that before the shift (0.027 g/g creatinine; p < 0.05). The GM of the CHZ metabolic ratio was 0.33 (0-9.3), with 40% of the subjects having ratios below the GM. However, the average CYP2E1 mRNA level in peripheral lymphocytes was 1.07 (0.30-3.08), and CYP2E1 mRNA levels within subjects correlated with the toluene exposure ratio (environmental toluene concentration:urinary hippuric acid concentration) (p = 0.014). Genotype did not alter the association between the toluene exposure ratio and mRNA content. In summary, with further validation, CYP2E1 mRNA content in peripheral lymphocytes could be a sensitive and noninvasive biomarker for the continuous monitoring of toluene effects in exposed persons.
Original languageEnglish
Pages (from-to)494-499
Number of pages6
JournalEnvironmental Health Perspectives
DOIs
Publication statusPublished - 1 Apr 2006
Externally publishedYes

Fingerprint

Cytochrome P-450 CYP2E1
Toluene
Occupational Exposure
Lymphocytes
Chlorzoxazone
Messenger RNA
Creatinine
Maximum Allowable Concentration
Genotype
5' Flanking Region
Environmental Exposure
Cytochrome P-450 Enzyme System
Biomarkers
Air

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Mendoza-Cantú, A., Castorena-Torres, F., Bermúdez de león, M., Cisneros, B., López-Carrillo, L., Rojas-García, A. E., ... Albores, A. (2006). Occupational toluene exposure induces cytochrome P450 2E1 mRNA expression in peripheral lymphocytes. Environmental Health Perspectives, 494-499. https://doi.org/10.1289/ehp.8192
Mendoza-Cantú, Ania ; Castorena-Torres, Fabiola ; Bermúdez de león, Mario ; Cisneros, Bulmaro ; López-Carrillo, Lizbeth ; Rojas-García, Aurora E. ; Aguilar-Salinas, Alberto ; Manno, Maurizio ; Albores, Arnulfo. / Occupational toluene exposure induces cytochrome P450 2E1 mRNA expression in peripheral lymphocytes. In: Environmental Health Perspectives. 2006 ; pp. 494-499.
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abstract = "Print workers are exposed to organic solvents, of which the systemic toxicant toluene is a main component. Toluene induces expression of cytochrome P450 2E1 (CYP2E1), an enzyme involved in its own metabolism and that of other protoxicants, including some procarcinogens. Therefore, we investigated the association between toluene exposure and the CYP2E1 response, as assessed by mRNA content in peripheral lymphocytes or the 6-hydroxychlorzoxazone (6OH-CHZ)/chlorzoxazone (CHZ) quotient (known as CHZ metabolic ratio) in plasma, and the role of genotype (5′-flanking region RsaI/PstI polymorphic sites) in 97 male print workers. The geometric mean (GM) of toluene concentration in the air was 52.80 ppm (10-760 ppm); 54{\%} of the study participants were exposed to toluene concentrations that exceeded the maximum permissible exposure level (MPEL). The GM of urinary hippuric acid at the end of a work shift (0.041 g/g creatinine) was elevated relative to that before the shift (0.027 g/g creatinine; p < 0.05). The GM of the CHZ metabolic ratio was 0.33 (0-9.3), with 40{\%} of the subjects having ratios below the GM. However, the average CYP2E1 mRNA level in peripheral lymphocytes was 1.07 (0.30-3.08), and CYP2E1 mRNA levels within subjects correlated with the toluene exposure ratio (environmental toluene concentration:urinary hippuric acid concentration) (p = 0.014). Genotype did not alter the association between the toluene exposure ratio and mRNA content. In summary, with further validation, CYP2E1 mRNA content in peripheral lymphocytes could be a sensitive and noninvasive biomarker for the continuous monitoring of toluene effects in exposed persons.",
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Mendoza-Cantú, A, Castorena-Torres, F, Bermúdez de león, M, Cisneros, B, López-Carrillo, L, Rojas-García, AE, Aguilar-Salinas, A, Manno, M & Albores, A 2006, 'Occupational toluene exposure induces cytochrome P450 2E1 mRNA expression in peripheral lymphocytes', Environmental Health Perspectives, pp. 494-499. https://doi.org/10.1289/ehp.8192

Occupational toluene exposure induces cytochrome P450 2E1 mRNA expression in peripheral lymphocytes. / Mendoza-Cantú, Ania; Castorena-Torres, Fabiola; Bermúdez de león, Mario; Cisneros, Bulmaro; López-Carrillo, Lizbeth; Rojas-García, Aurora E.; Aguilar-Salinas, Alberto; Manno, Maurizio; Albores, Arnulfo.

In: Environmental Health Perspectives, 01.04.2006, p. 494-499.

Research output: Contribution to journalArticle

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T1 - Occupational toluene exposure induces cytochrome P450 2E1 mRNA expression in peripheral lymphocytes

AU - Mendoza-Cantú, Ania

AU - Castorena-Torres, Fabiola

AU - Bermúdez de león, Mario

AU - Cisneros, Bulmaro

AU - López-Carrillo, Lizbeth

AU - Rojas-García, Aurora E.

AU - Aguilar-Salinas, Alberto

AU - Manno, Maurizio

AU - Albores, Arnulfo

PY - 2006/4/1

Y1 - 2006/4/1

N2 - Print workers are exposed to organic solvents, of which the systemic toxicant toluene is a main component. Toluene induces expression of cytochrome P450 2E1 (CYP2E1), an enzyme involved in its own metabolism and that of other protoxicants, including some procarcinogens. Therefore, we investigated the association between toluene exposure and the CYP2E1 response, as assessed by mRNA content in peripheral lymphocytes or the 6-hydroxychlorzoxazone (6OH-CHZ)/chlorzoxazone (CHZ) quotient (known as CHZ metabolic ratio) in plasma, and the role of genotype (5′-flanking region RsaI/PstI polymorphic sites) in 97 male print workers. The geometric mean (GM) of toluene concentration in the air was 52.80 ppm (10-760 ppm); 54% of the study participants were exposed to toluene concentrations that exceeded the maximum permissible exposure level (MPEL). The GM of urinary hippuric acid at the end of a work shift (0.041 g/g creatinine) was elevated relative to that before the shift (0.027 g/g creatinine; p < 0.05). The GM of the CHZ metabolic ratio was 0.33 (0-9.3), with 40% of the subjects having ratios below the GM. However, the average CYP2E1 mRNA level in peripheral lymphocytes was 1.07 (0.30-3.08), and CYP2E1 mRNA levels within subjects correlated with the toluene exposure ratio (environmental toluene concentration:urinary hippuric acid concentration) (p = 0.014). Genotype did not alter the association between the toluene exposure ratio and mRNA content. In summary, with further validation, CYP2E1 mRNA content in peripheral lymphocytes could be a sensitive and noninvasive biomarker for the continuous monitoring of toluene effects in exposed persons.

AB - Print workers are exposed to organic solvents, of which the systemic toxicant toluene is a main component. Toluene induces expression of cytochrome P450 2E1 (CYP2E1), an enzyme involved in its own metabolism and that of other protoxicants, including some procarcinogens. Therefore, we investigated the association between toluene exposure and the CYP2E1 response, as assessed by mRNA content in peripheral lymphocytes or the 6-hydroxychlorzoxazone (6OH-CHZ)/chlorzoxazone (CHZ) quotient (known as CHZ metabolic ratio) in plasma, and the role of genotype (5′-flanking region RsaI/PstI polymorphic sites) in 97 male print workers. The geometric mean (GM) of toluene concentration in the air was 52.80 ppm (10-760 ppm); 54% of the study participants were exposed to toluene concentrations that exceeded the maximum permissible exposure level (MPEL). The GM of urinary hippuric acid at the end of a work shift (0.041 g/g creatinine) was elevated relative to that before the shift (0.027 g/g creatinine; p < 0.05). The GM of the CHZ metabolic ratio was 0.33 (0-9.3), with 40% of the subjects having ratios below the GM. However, the average CYP2E1 mRNA level in peripheral lymphocytes was 1.07 (0.30-3.08), and CYP2E1 mRNA levels within subjects correlated with the toluene exposure ratio (environmental toluene concentration:urinary hippuric acid concentration) (p = 0.014). Genotype did not alter the association between the toluene exposure ratio and mRNA content. In summary, with further validation, CYP2E1 mRNA content in peripheral lymphocytes could be a sensitive and noninvasive biomarker for the continuous monitoring of toluene effects in exposed persons.

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DO - 10.1289/ehp.8192

M3 - Article

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JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

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