NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts

Paul Delgado-Olguín, Haydeé Rosas-Vargas, Félix Recillas-Targa, Alejandro Zentella-Dehesa, Mario Bermúdez De León, Bulmaro Cisneros, Fabio Salamanca, Ramón Coral-Vázquez

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

α-Sarcoglycan striated muscle-specific protein is a member of the sarcoglycan-sarcospan complex. Positive and negative transcriptional regulation of sarcoglycan genes are important in sarcoglycan's intracellular localization and sarcolemmal stability. In the present work we assessed the function of NFI transcription factors in the regulation of α-sarcoglycan promoter through the C2C12 cell line differentiation. NFI factors act alternatively as activators and negative modulators of α-sarcoglycan promoter activity. In myoblasts NFI-A1.1 and NFI-B2 are activators, whereas NFI-C2 and NFI-X2 are negative regulators. In myotubes, all NFI members are activators, being NFI-C2 the less potent. We identified the α-sarcoglycan promoter NFI-C2 response element by testing progressive deletion constructs and point mutations in C2C12 cells over-expressing NFI-C2. Gel-shift and chromatin immunoprecipitation experiments demonstrated that NFI factors are indeed interacting in vitro and in vivo with the binding sequence. These results suggest a NFI role in C2C12 cell differentiation. © 2004 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)1032-1039
Number of pages8
JournalBiochemical and Biophysical Research Communications
DOIs
Publication statusPublished - 2 Jul 2004
Externally publishedYes

Fingerprint

Sarcoglycans
Myoblasts
Cell Differentiation
NFI Transcription Factors
Striated Muscle
Muscle Proteins
Sequence Deletion
Chromatin Immunoprecipitation
Skeletal Muscle Fibers
Response Elements
Point Mutation
Modulators
Chromatin
Muscle
Genes
Gels
Cells
Cell Line
Testing

Cite this

Delgado-Olguín, P., Rosas-Vargas, H., Recillas-Targa, F., Zentella-Dehesa, A., De León, M. B., Cisneros, B., ... Coral-Vázquez, R. (2004). NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts. Biochemical and Biophysical Research Communications, 1032-1039. https://doi.org/10.1016/j.bbrc.2004.05.074
Delgado-Olguín, Paul ; Rosas-Vargas, Haydeé ; Recillas-Targa, Félix ; Zentella-Dehesa, Alejandro ; De León, Mario Bermúdez ; Cisneros, Bulmaro ; Salamanca, Fabio ; Coral-Vázquez, Ramón. / NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts. In: Biochemical and Biophysical Research Communications. 2004 ; pp. 1032-1039.
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abstract = "α-Sarcoglycan striated muscle-specific protein is a member of the sarcoglycan-sarcospan complex. Positive and negative transcriptional regulation of sarcoglycan genes are important in sarcoglycan's intracellular localization and sarcolemmal stability. In the present work we assessed the function of NFI transcription factors in the regulation of α-sarcoglycan promoter through the C2C12 cell line differentiation. NFI factors act alternatively as activators and negative modulators of α-sarcoglycan promoter activity. In myoblasts NFI-A1.1 and NFI-B2 are activators, whereas NFI-C2 and NFI-X2 are negative regulators. In myotubes, all NFI members are activators, being NFI-C2 the less potent. We identified the α-sarcoglycan promoter NFI-C2 response element by testing progressive deletion constructs and point mutations in C2C12 cells over-expressing NFI-C2. Gel-shift and chromatin immunoprecipitation experiments demonstrated that NFI factors are indeed interacting in vitro and in vivo with the binding sequence. These results suggest a NFI role in C2C12 cell differentiation. {\circledC} 2004 Elsevier Inc. All rights reserved.",
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Delgado-Olguín, P, Rosas-Vargas, H, Recillas-Targa, F, Zentella-Dehesa, A, De León, MB, Cisneros, B, Salamanca, F & Coral-Vázquez, R 2004, 'NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts', Biochemical and Biophysical Research Communications, pp. 1032-1039. https://doi.org/10.1016/j.bbrc.2004.05.074

NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts. / Delgado-Olguín, Paul; Rosas-Vargas, Haydeé; Recillas-Targa, Félix; Zentella-Dehesa, Alejandro; De León, Mario Bermúdez; Cisneros, Bulmaro; Salamanca, Fabio; Coral-Vázquez, Ramón.

In: Biochemical and Biophysical Research Communications, 02.07.2004, p. 1032-1039.

Research output: Contribution to journalArticle

TY - JOUR

T1 - NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts

AU - Delgado-Olguín, Paul

AU - Rosas-Vargas, Haydeé

AU - Recillas-Targa, Félix

AU - Zentella-Dehesa, Alejandro

AU - De León, Mario Bermúdez

AU - Cisneros, Bulmaro

AU - Salamanca, Fabio

AU - Coral-Vázquez, Ramón

PY - 2004/7/2

Y1 - 2004/7/2

N2 - α-Sarcoglycan striated muscle-specific protein is a member of the sarcoglycan-sarcospan complex. Positive and negative transcriptional regulation of sarcoglycan genes are important in sarcoglycan's intracellular localization and sarcolemmal stability. In the present work we assessed the function of NFI transcription factors in the regulation of α-sarcoglycan promoter through the C2C12 cell line differentiation. NFI factors act alternatively as activators and negative modulators of α-sarcoglycan promoter activity. In myoblasts NFI-A1.1 and NFI-B2 are activators, whereas NFI-C2 and NFI-X2 are negative regulators. In myotubes, all NFI members are activators, being NFI-C2 the less potent. We identified the α-sarcoglycan promoter NFI-C2 response element by testing progressive deletion constructs and point mutations in C2C12 cells over-expressing NFI-C2. Gel-shift and chromatin immunoprecipitation experiments demonstrated that NFI factors are indeed interacting in vitro and in vivo with the binding sequence. These results suggest a NFI role in C2C12 cell differentiation. © 2004 Elsevier Inc. All rights reserved.

AB - α-Sarcoglycan striated muscle-specific protein is a member of the sarcoglycan-sarcospan complex. Positive and negative transcriptional regulation of sarcoglycan genes are important in sarcoglycan's intracellular localization and sarcolemmal stability. In the present work we assessed the function of NFI transcription factors in the regulation of α-sarcoglycan promoter through the C2C12 cell line differentiation. NFI factors act alternatively as activators and negative modulators of α-sarcoglycan promoter activity. In myoblasts NFI-A1.1 and NFI-B2 are activators, whereas NFI-C2 and NFI-X2 are negative regulators. In myotubes, all NFI members are activators, being NFI-C2 the less potent. We identified the α-sarcoglycan promoter NFI-C2 response element by testing progressive deletion constructs and point mutations in C2C12 cells over-expressing NFI-C2. Gel-shift and chromatin immunoprecipitation experiments demonstrated that NFI factors are indeed interacting in vitro and in vivo with the binding sequence. These results suggest a NFI role in C2C12 cell differentiation. © 2004 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.bbrc.2004.05.074

DO - 10.1016/j.bbrc.2004.05.074

M3 - Article

SP - 1032

EP - 1039

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

ER -