NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts

Paul Delgado-Olguín; Haydeé Rosas-Vargas; Félix Recillas-Targa; Alejandro Zentella-Dehesa; Mario Bermúdez De León; Bulmaro Cisneros; Fabio Salamanca; Ramón Coral-Vázquez

doi:10.1016/j.bbrc.2004.05.074

NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts

Paul Delgado-Olguín, Haydeé Rosas-Vargas, Félix Recillas-Targa, Alejandro Zentella-Dehesa, Mario Bermúdez De León, Bulmaro Cisneros, Fabio Salamanca, Ramón Coral-Vázquez

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

α-Sarcoglycan striated muscle-specific protein is a member of the sarcoglycan-sarcospan complex. Positive and negative transcriptional regulation of sarcoglycan genes are important in sarcoglycan's intracellular localization and sarcolemmal stability. In the present work we assessed the function of NFI transcription factors in the regulation of α-sarcoglycan promoter through the C2C12 cell line differentiation. NFI factors act alternatively as activators and negative modulators of α-sarcoglycan promoter activity. In myoblasts NFI-A1.1 and NFI-B2 are activators, whereas NFI-C2 and NFI-X2 are negative regulators. In myotubes, all NFI members are activators, being NFI-C2 the less potent. We identified the α-sarcoglycan promoter NFI-C2 response element by testing progressive deletion constructs and point mutations in C2C12 cells over-expressing NFI-C2. Gel-shift and chromatin immunoprecipitation experiments demonstrated that NFI factors are indeed interacting in vitro and in vivo with the binding sequence. These results suggest a NFI role in C2C12 cell differentiation.

Original language	English
Pages (from-to)	1032-1039
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	319
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2004.05.074
Publication status	Published - 2 Jul 2004
Externally published	Yes

Bibliographical note

Funding Information:
P. Delgado-Olguı́n is a Ph.D. student supported by the Consejo Nacional de Ciencia y Tecnologı́a (CONACyT) through the Grant 158524, by the Dirección General de Estudios de Posgrado, UNAM, and by the IMSS fellowship award. We gratefully acknowledge funding support from Association Française contre les Myopathies (Grant MNM 2000 Group 1). This work was partially supported by CONACyT (Grant 33863-N and 42653-Q), PAPIIT-DGAPA (IN203200 and IN209403), The Third World Academy of Sciences (TWAS, Grant 01-055RG/BIO/LA), and the Fundación Miguel Alemán to F.R.T. We thank Georgina Guerrero Avendaño for excellent technical assistance.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2004.05.074

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@article{351e7c5dc1d4477ab05feec320d0845b,

title = "NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts",

abstract = "α-Sarcoglycan striated muscle-specific protein is a member of the sarcoglycan-sarcospan complex. Positive and negative transcriptional regulation of sarcoglycan genes are important in sarcoglycan's intracellular localization and sarcolemmal stability. In the present work we assessed the function of NFI transcription factors in the regulation of α-sarcoglycan promoter through the C2C12 cell line differentiation. NFI factors act alternatively as activators and negative modulators of α-sarcoglycan promoter activity. In myoblasts NFI-A1.1 and NFI-B2 are activators, whereas NFI-C2 and NFI-X2 are negative regulators. In myotubes, all NFI members are activators, being NFI-C2 the less potent. We identified the α-sarcoglycan promoter NFI-C2 response element by testing progressive deletion constructs and point mutations in C2C12 cells over-expressing NFI-C2. Gel-shift and chromatin immunoprecipitation experiments demonstrated that NFI factors are indeed interacting in vitro and in vivo with the binding sequence. These results suggest a NFI role in C2C12 cell differentiation.",

author = "Paul Delgado-Olgu{\'i}n and Hayde{\'e} Rosas-Vargas and F{\'e}lix Recillas-Targa and Alejandro Zentella-Dehesa and {De Le{\'o}n}, {Mario Berm{\'u}dez} and Bulmaro Cisneros and Fabio Salamanca and Ram{\'o}n Coral-V{\'a}zquez",

note = "Funding Information: P. Delgado-Olgu{\'ı}n is a Ph.D. student supported by the Consejo Nacional de Ciencia y Tecnolog{\'ı}a (CONACyT) through the Grant 158524, by the Direcci{\'o}n General de Estudios de Posgrado, UNAM, and by the IMSS fellowship award. We gratefully acknowledge funding support from Association Fran{\c c}aise contre les Myopathies (Grant MNM 2000 Group 1). This work was partially supported by CONACyT (Grant 33863-N and 42653-Q), PAPIIT-DGAPA (IN203200 and IN209403), The Third World Academy of Sciences (TWAS, Grant 01-055RG/BIO/LA), and the Fundaci{\'o}n Miguel Alem{\'a}n to F.R.T. We thank Georgina Guerrero Avenda{\~n}o for excellent technical assistance. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2004",

month = jul,

day = "2",

doi = "10.1016/j.bbrc.2004.05.074",

language = "English",

volume = "319",

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T1 - NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts

AU - Delgado-Olguín, Paul

AU - Rosas-Vargas, Haydeé

AU - Recillas-Targa, Félix

AU - Zentella-Dehesa, Alejandro

AU - De León, Mario Bermúdez

AU - Cisneros, Bulmaro

AU - Salamanca, Fabio

AU - Coral-Vázquez, Ramón

N1 - Funding Information: P. Delgado-Olguı́n is a Ph.D. student supported by the Consejo Nacional de Ciencia y Tecnologı́a (CONACyT) through the Grant 158524, by the Dirección General de Estudios de Posgrado, UNAM, and by the IMSS fellowship award. We gratefully acknowledge funding support from Association Française contre les Myopathies (Grant MNM 2000 Group 1). This work was partially supported by CONACyT (Grant 33863-N and 42653-Q), PAPIIT-DGAPA (IN203200 and IN209403), The Third World Academy of Sciences (TWAS, Grant 01-055RG/BIO/LA), and the Fundación Miguel Alemán to F.R.T. We thank Georgina Guerrero Avendaño for excellent technical assistance. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2004/7/2

Y1 - 2004/7/2

N2 - α-Sarcoglycan striated muscle-specific protein is a member of the sarcoglycan-sarcospan complex. Positive and negative transcriptional regulation of sarcoglycan genes are important in sarcoglycan's intracellular localization and sarcolemmal stability. In the present work we assessed the function of NFI transcription factors in the regulation of α-sarcoglycan promoter through the C2C12 cell line differentiation. NFI factors act alternatively as activators and negative modulators of α-sarcoglycan promoter activity. In myoblasts NFI-A1.1 and NFI-B2 are activators, whereas NFI-C2 and NFI-X2 are negative regulators. In myotubes, all NFI members are activators, being NFI-C2 the less potent. We identified the α-sarcoglycan promoter NFI-C2 response element by testing progressive deletion constructs and point mutations in C2C12 cells over-expressing NFI-C2. Gel-shift and chromatin immunoprecipitation experiments demonstrated that NFI factors are indeed interacting in vitro and in vivo with the binding sequence. These results suggest a NFI role in C2C12 cell differentiation.

AB - α-Sarcoglycan striated muscle-specific protein is a member of the sarcoglycan-sarcospan complex. Positive and negative transcriptional regulation of sarcoglycan genes are important in sarcoglycan's intracellular localization and sarcolemmal stability. In the present work we assessed the function of NFI transcription factors in the regulation of α-sarcoglycan promoter through the C2C12 cell line differentiation. NFI factors act alternatively as activators and negative modulators of α-sarcoglycan promoter activity. In myoblasts NFI-A1.1 and NFI-B2 are activators, whereas NFI-C2 and NFI-X2 are negative regulators. In myotubes, all NFI members are activators, being NFI-C2 the less potent. We identified the α-sarcoglycan promoter NFI-C2 response element by testing progressive deletion constructs and point mutations in C2C12 cells over-expressing NFI-C2. Gel-shift and chromatin immunoprecipitation experiments demonstrated that NFI factors are indeed interacting in vitro and in vivo with the binding sequence. These results suggest a NFI role in C2C12 cell differentiation.

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M3 - Article

SN - 0006-291X

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EP - 1039

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

NFI-C2 negatively regulates α-sarcoglycan promoter activity in C2C12 myoblasts

Abstract

Bibliographical note

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