Neuron-like phenotypic changes in pancreatic β-cells induced by NGF, FGF, and dbcAMP

Román Vidaltamayo, Ma Carmen Sánchez-Soto, Tamara Rosenbaum, Teresa Martínez-Merlos, Marcia Hiriart

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Abstract

We studied the effects of nerve growth factor (NGF), fibroblast growth factor (FGF), and dibutyryl-cAMP (dbcAMP) on rat pancreatic β-cell morphology and of NGF and dbcAMP on insulin secretion. After 2 wk in culture, nearly 3% of β-cells extended neurite-like processes spontaneously; when cells were treated with NGF, almost 30% of them extended processes. In the presence of dbcAMP, almost all β-cells flattened, and the extension of neurite-like processes was more pronounced in fetal than in adult cells. The most prominent effect, regardless of age, was observed in cells treated with NGF and dbcAMP together, since the percentage of neurite-like bearing β-cells increased to 50%. β-cells cultured under these conditions maintained their immunoreactivity to insulin and nearly all β-cells and their neurite-like processes were also positive to GABA, tubulin, tau protein, and N-CAM. FGF increased the percentage of adult β-cells bearing neurite-like processes to 13%, and FGF and dbcAMP applied together to 40%. β-cells treated with NGF and dbcAMP for 5 to 7 d preserved their capability to secrete the hormone in response to different extracellular glucose concentrations. Insulin secretion of dbcAMP-treated β-cells was 2.5-fold higher than in control cells. NGF-treated cells were able to discriminate between different glucose concentrations, a property lost in control cells with time in culture.
Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalEndocrine
Publication statusPublished - 1 Feb 1996
Externally publishedYes

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Fibroblast Growth Factors
Nerve Growth Factor
Neurons
Neurites
Insulin
Glucose
tau Proteins
Tubulin
gamma-Aminobutyric Acid
Cultured Cells

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Vidaltamayo, R., Sánchez-Soto, M. C., Rosenbaum, T., Martínez-Merlos, T., & Hiriart, M. (1996). Neuron-like phenotypic changes in pancreatic β-cells induced by NGF, FGF, and dbcAMP. Endocrine, 19-26.
Vidaltamayo, Román ; Sánchez-Soto, Ma Carmen ; Rosenbaum, Tamara ; Martínez-Merlos, Teresa ; Hiriart, Marcia. / Neuron-like phenotypic changes in pancreatic β-cells induced by NGF, FGF, and dbcAMP. In: Endocrine. 1996 ; pp. 19-26.
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Vidaltamayo, R, Sánchez-Soto, MC, Rosenbaum, T, Martínez-Merlos, T & Hiriart, M 1996, 'Neuron-like phenotypic changes in pancreatic β-cells induced by NGF, FGF, and dbcAMP', Endocrine, pp. 19-26.

Neuron-like phenotypic changes in pancreatic β-cells induced by NGF, FGF, and dbcAMP. / Vidaltamayo, Román; Sánchez-Soto, Ma Carmen; Rosenbaum, Tamara; Martínez-Merlos, Teresa; Hiriart, Marcia.

In: Endocrine, 01.02.1996, p. 19-26.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Neuron-like phenotypic changes in pancreatic β-cells induced by NGF, FGF, and dbcAMP

AU - Vidaltamayo, Román

AU - Sánchez-Soto, Ma Carmen

AU - Rosenbaum, Tamara

AU - Martínez-Merlos, Teresa

AU - Hiriart, Marcia

PY - 1996/2/1

Y1 - 1996/2/1

N2 - We studied the effects of nerve growth factor (NGF), fibroblast growth factor (FGF), and dibutyryl-cAMP (dbcAMP) on rat pancreatic β-cell morphology and of NGF and dbcAMP on insulin secretion. After 2 wk in culture, nearly 3% of β-cells extended neurite-like processes spontaneously; when cells were treated with NGF, almost 30% of them extended processes. In the presence of dbcAMP, almost all β-cells flattened, and the extension of neurite-like processes was more pronounced in fetal than in adult cells. The most prominent effect, regardless of age, was observed in cells treated with NGF and dbcAMP together, since the percentage of neurite-like bearing β-cells increased to 50%. β-cells cultured under these conditions maintained their immunoreactivity to insulin and nearly all β-cells and their neurite-like processes were also positive to GABA, tubulin, tau protein, and N-CAM. FGF increased the percentage of adult β-cells bearing neurite-like processes to 13%, and FGF and dbcAMP applied together to 40%. β-cells treated with NGF and dbcAMP for 5 to 7 d preserved their capability to secrete the hormone in response to different extracellular glucose concentrations. Insulin secretion of dbcAMP-treated β-cells was 2.5-fold higher than in control cells. NGF-treated cells were able to discriminate between different glucose concentrations, a property lost in control cells with time in culture.

AB - We studied the effects of nerve growth factor (NGF), fibroblast growth factor (FGF), and dibutyryl-cAMP (dbcAMP) on rat pancreatic β-cell morphology and of NGF and dbcAMP on insulin secretion. After 2 wk in culture, nearly 3% of β-cells extended neurite-like processes spontaneously; when cells were treated with NGF, almost 30% of them extended processes. In the presence of dbcAMP, almost all β-cells flattened, and the extension of neurite-like processes was more pronounced in fetal than in adult cells. The most prominent effect, regardless of age, was observed in cells treated with NGF and dbcAMP together, since the percentage of neurite-like bearing β-cells increased to 50%. β-cells cultured under these conditions maintained their immunoreactivity to insulin and nearly all β-cells and their neurite-like processes were also positive to GABA, tubulin, tau protein, and N-CAM. FGF increased the percentage of adult β-cells bearing neurite-like processes to 13%, and FGF and dbcAMP applied together to 40%. β-cells treated with NGF and dbcAMP for 5 to 7 d preserved their capability to secrete the hormone in response to different extracellular glucose concentrations. Insulin secretion of dbcAMP-treated β-cells was 2.5-fold higher than in control cells. NGF-treated cells were able to discriminate between different glucose concentrations, a property lost in control cells with time in culture.

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SN - 1355-008X

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Vidaltamayo R, Sánchez-Soto MC, Rosenbaum T, Martínez-Merlos T, Hiriart M. Neuron-like phenotypic changes in pancreatic β-cells induced by NGF, FGF, and dbcAMP. Endocrine. 1996 Feb 1;19-26.