Myotonic dystrophy type 1-associated CTG repeats disturb the expression and subcellular distribution of microtubule-associated proteins MAP1A, MAP2, and MAP6/STOP in PC12 cells

Prisiliana Velázquez-Bernardino; Francisco García-Sierra; Oscar Hernández-Hernández; Mario Bermúdez De León; Geneviève Gourdon; Mário Gomes-Pereira; Bulmaro Cisneros

doi:10.1007/s11033-011-0753-y

Myotonic dystrophy type 1-associated CTG repeats disturb the expression and subcellular distribution of microtubule-associated proteins MAP1A, MAP2, and MAP6/STOP in PC12 cells

Prisiliana Velázquez-Bernardino, Francisco García-Sierra, Oscar Hernández-Hernández, Mario Bermúdez De León, Geneviève Gourdon, Mário Gomes-Pereira, Bulmaro Cisneros

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

To study the effect of DM1-associated CTG repeats on neuronal function, we developed a PC12 cell-based model that constitutively expresses the DMPK gene 3′-untranslated region with 90 CTG repeats (CTG90 cells). As CTG90 cells exhibit impaired neurite outgrowth and as microtubule-associated proteins (MAPs) are crucial for microtubule stability, we analyzed whether MAPs are a target of CTG repeats. NGF induces mRNA expression of Map2, Map1a and Map6 in control cells (PC12 cells transfected with the empty vector), but this induction is abolished for Map2 and Map1a in CTG90 cells. MAP2 and MAP6/STOP proteins decrease in NGF-treated CTG90 cells, whereas MAP1A increases. Data suggest that CTG repeats might alter somehow the expression of MAPs, which appears to be related with CTG90 cell-deficient neurite outgrowth. Decreased MAP2 levels found in the hippocampus of a DM1 mouse model indicates that targeting of MAPs expression by CTG repeats might be relevant to DM1.

Original language	English
Pages (from-to)	415-424
Number of pages	10
Journal	Molecular Biology Reports
Volume	39
Issue number	1
DOIs	https://doi.org/10.1007/s11033-011-0753-y
Publication status	Published - 1 Jan 2012
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments This study was supported by the Muscular Dystrophy Association, Inc. Grant No. MDA-3693. Prisiliana Vélazquez-Bernardino PhD student was supported by CONACyT, control number: 185951. We are in debt with Dr. Christophe Bosc (INSERM, France) and Dr. Manuel Hernández (Cinvestav, México) for providing us with the anti-MAP6/STOP and anti-actin antibodies, respectively.

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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10.1007/s11033-011-0753-y

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Cite this

Velázquez-Bernardino, P., García-Sierra, F., Hernández-Hernández, O., Bermúdez De León, M., Gourdon, G., Gomes-Pereira, M., & Cisneros, B. (2012). Myotonic dystrophy type 1-associated CTG repeats disturb the expression and subcellular distribution of microtubule-associated proteins MAP1A, MAP2, and MAP6/STOP in PC12 cells. Molecular Biology Reports, 39(1), 415-424. https://doi.org/10.1007/s11033-011-0753-y

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title = "Myotonic dystrophy type 1-associated CTG repeats disturb the expression and subcellular distribution of microtubule-associated proteins MAP1A, MAP2, and MAP6/STOP in PC12 cells",

abstract = "To study the effect of DM1-associated CTG repeats on neuronal function, we developed a PC12 cell-based model that constitutively expresses the DMPK gene 3′-untranslated region with 90 CTG repeats (CTG90 cells). As CTG90 cells exhibit impaired neurite outgrowth and as microtubule-associated proteins (MAPs) are crucial for microtubule stability, we analyzed whether MAPs are a target of CTG repeats. NGF induces mRNA expression of Map2, Map1a and Map6 in control cells (PC12 cells transfected with the empty vector), but this induction is abolished for Map2 and Map1a in CTG90 cells. MAP2 and MAP6/STOP proteins decrease in NGF-treated CTG90 cells, whereas MAP1A increases. Data suggest that CTG repeats might alter somehow the expression of MAPs, which appears to be related with CTG90 cell-deficient neurite outgrowth. Decreased MAP2 levels found in the hippocampus of a DM1 mouse model indicates that targeting of MAPs expression by CTG repeats might be relevant to DM1.",

author = "Prisiliana Vel{\'a}zquez-Bernardino and Francisco Garc{\'i}a-Sierra and Oscar Hern{\'a}ndez-Hern{\'a}ndez and {Berm{\'u}dez De Le{\'o}n}, Mario and Genevi{\`e}ve Gourdon and M{\'a}rio Gomes-Pereira and Bulmaro Cisneros",

note = "Funding Information: Acknowledgments This study was supported by the Muscular Dystrophy Association, Inc. Grant No. MDA-3693. Prisiliana V{\'e}lazquez-Bernardino PhD student was supported by CONACyT, control number: 185951. We are in debt with Dr. Christophe Bosc (INSERM, France) and Dr. Manuel Hern{\'a}ndez (Cinvestav, M{\'e}xico) for providing us with the anti-MAP6/STOP and anti-actin antibodies, respectively. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2012",

month = jan,

day = "1",

doi = "10.1007/s11033-011-0753-y",

language = "English",

volume = "39",

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Velázquez-Bernardino, P, García-Sierra, F, Hernández-Hernández, O, Bermúdez De León, M, Gourdon, G, Gomes-Pereira, M & Cisneros, B 2012, 'Myotonic dystrophy type 1-associated CTG repeats disturb the expression and subcellular distribution of microtubule-associated proteins MAP1A, MAP2, and MAP6/STOP in PC12 cells', Molecular Biology Reports, vol. 39, no. 1, pp. 415-424. https://doi.org/10.1007/s11033-011-0753-y

Myotonic dystrophy type 1-associated CTG repeats disturb the expression and subcellular distribution of microtubule-associated proteins MAP1A, MAP2, and MAP6/STOP in PC12 cells. / Velázquez-Bernardino, Prisiliana; García-Sierra, Francisco; Hernández-Hernández, Oscar et al.
In: Molecular Biology Reports, Vol. 39, No. 1, 01.01.2012, p. 415-424.

Research output: Contribution to journal › Article › peer-review

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T1 - Myotonic dystrophy type 1-associated CTG repeats disturb the expression and subcellular distribution of microtubule-associated proteins MAP1A, MAP2, and MAP6/STOP in PC12 cells

AU - Velázquez-Bernardino, Prisiliana

AU - García-Sierra, Francisco

AU - Hernández-Hernández, Oscar

AU - Bermúdez De León, Mario

AU - Gourdon, Geneviève

AU - Gomes-Pereira, Mário

AU - Cisneros, Bulmaro

N1 - Funding Information: Acknowledgments This study was supported by the Muscular Dystrophy Association, Inc. Grant No. MDA-3693. Prisiliana Vélazquez-Bernardino PhD student was supported by CONACyT, control number: 185951. We are in debt with Dr. Christophe Bosc (INSERM, France) and Dr. Manuel Hernández (Cinvestav, México) for providing us with the anti-MAP6/STOP and anti-actin antibodies, respectively. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - To study the effect of DM1-associated CTG repeats on neuronal function, we developed a PC12 cell-based model that constitutively expresses the DMPK gene 3′-untranslated region with 90 CTG repeats (CTG90 cells). As CTG90 cells exhibit impaired neurite outgrowth and as microtubule-associated proteins (MAPs) are crucial for microtubule stability, we analyzed whether MAPs are a target of CTG repeats. NGF induces mRNA expression of Map2, Map1a and Map6 in control cells (PC12 cells transfected with the empty vector), but this induction is abolished for Map2 and Map1a in CTG90 cells. MAP2 and MAP6/STOP proteins decrease in NGF-treated CTG90 cells, whereas MAP1A increases. Data suggest that CTG repeats might alter somehow the expression of MAPs, which appears to be related with CTG90 cell-deficient neurite outgrowth. Decreased MAP2 levels found in the hippocampus of a DM1 mouse model indicates that targeting of MAPs expression by CTG repeats might be relevant to DM1.

AB - To study the effect of DM1-associated CTG repeats on neuronal function, we developed a PC12 cell-based model that constitutively expresses the DMPK gene 3′-untranslated region with 90 CTG repeats (CTG90 cells). As CTG90 cells exhibit impaired neurite outgrowth and as microtubule-associated proteins (MAPs) are crucial for microtubule stability, we analyzed whether MAPs are a target of CTG repeats. NGF induces mRNA expression of Map2, Map1a and Map6 in control cells (PC12 cells transfected with the empty vector), but this induction is abolished for Map2 and Map1a in CTG90 cells. MAP2 and MAP6/STOP proteins decrease in NGF-treated CTG90 cells, whereas MAP1A increases. Data suggest that CTG repeats might alter somehow the expression of MAPs, which appears to be related with CTG90 cell-deficient neurite outgrowth. Decreased MAP2 levels found in the hippocampus of a DM1 mouse model indicates that targeting of MAPs expression by CTG repeats might be relevant to DM1.

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Velázquez-Bernardino P, García-Sierra F, Hernández-Hernández O, Bermúdez De León M, Gourdon G, Gomes-Pereira M et al. Myotonic dystrophy type 1-associated CTG repeats disturb the expression and subcellular distribution of microtubule-associated proteins MAP1A, MAP2, and MAP6/STOP in PC12 cells. Molecular Biology Reports. 2012 Jan 1;39(1):415-424. doi: 10.1007/s11033-011-0753-y

Myotonic dystrophy type 1-associated CTG repeats disturb the expression and subcellular distribution of microtubule-associated proteins MAP1A, MAP2, and MAP6/STOP in PC12 cells

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