Molecular identification of unusual Mycetoma agents isolated from patients in Venezuela

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mycetoma is a chronic granulomatous, subcutaneous disease endemic in tropical and subtropical countries. It is currently a health problem in rural areas of Africa, Asia and South America. Nine cases of mycetoma were analysed in a retrospective study. All isolates were identified by morphological features. The level of species identification was reached by molecular tools. Definitive identification of fungi was performed using sequence analysis of the ITS of the ribosomal DNA region and the ribosomal large-subunit D1/D2. Identification of actinomycetes was accomplished by the 16S rRNA gene sequence. Six unusual clinical isolates were identified: Aspergillus ustus, Cyphellophora oxyspora, Exophiala oligosperma, Madurella pseudomycetomatis, Nocardia farcinica and Nocardia wallacei. The prevalence of mycetoma in Venezuela remains unknown. This study represents the first report in the literature of mycetoma caused by unusual pathogens identified by molecular techniques.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalMycoses
Volume60
Issue number2
DOIs
Publication statusPublished - 1 Feb 2017

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Mycetoma
Venezuela
Proxy
Nocardia
Madurella
Exophiala
Large Ribosome Subunits
Chronic Granulomatous Disease
Actinobacteria
South America
Aspergillus
Ribosomal DNA
rRNA Genes
Sequence Analysis
Fungi
Retrospective Studies
Health

All Science Journal Classification (ASJC) codes

  • Dermatology
  • Infectious Diseases

Cite this

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title = "Molecular identification of unusual Mycetoma agents isolated from patients in Venezuela",
abstract = "Mycetoma is a chronic granulomatous, subcutaneous disease endemic in tropical and subtropical countries. It is currently a health problem in rural areas of Africa, Asia and South America. Nine cases of mycetoma were analysed in a retrospective study. All isolates were identified by morphological features. The level of species identification was reached by molecular tools. Definitive identification of fungi was performed using sequence analysis of the ITS of the ribosomal DNA region and the ribosomal large-subunit D1/D2. Identification of actinomycetes was accomplished by the 16S rRNA gene sequence. Six unusual clinical isolates were identified: Aspergillus ustus, Cyphellophora oxyspora, Exophiala oligosperma, Madurella pseudomycetomatis, Nocardia farcinica and Nocardia wallacei. The prevalence of mycetoma in Venezuela remains unknown. This study represents the first report in the literature of mycetoma caused by unusual pathogens identified by molecular techniques.",
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Molecular identification of unusual Mycetoma agents isolated from patients in Venezuela. / Rojas, Olga C.; León-Cachón, Rafael B.R.; Moreno-Treviño, Maria; González, Gloria M.

In: Mycoses, Vol. 60, No. 2, 01.02.2017, p. 129-135.

Research output: Contribution to journalArticle

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