Mg2+, Mn2+, and Co2+ stimulate Entamoeba histolytica to produce chitin-like material

S. Said-Fernández, E. Campos-Góngora, F. González-Salazar, H. G. Martinez-Rodriguez, J. Vargas-Villarreal, J. M. Viader-Salvadó

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The mechanism of Entamoeba histolytica cyst cell wall synthesis is not well understood. Previous research has shown that cystlike structures formed in the presence of chitin synthase cofactors (Mg2+, Mn2+, and Co2+) resist 1% sodium dodecyl sulfate lysis (RCLS), whereas those formed in the absence of cofactors (CLS) do not, and trophozoites are immediately destroyed. This suggests that E. histolytica is able to synthesize chitin, initiating a differentiation process under axenic conditions. To test this hypothesis, polysaccharide hydrolysates from E. histolytica trophozoites, CLS, or RCLS were analyzed with high-performance liquid chromatography. The major components found in all 3 preparations were N-acetylglucosamine (NAG) and glucose (GLC), with RCLS possessing 129 and 180 times more NAG and 2.4 and 2.0 more GLC than trophozoites and CLS, respectively. After 36 hr of incubation with chitinase (16 U/ml) in a hypotonic medium (50 mOsm/kg), 68% of RCLS was lysed, and 100% lost affinity for calcofluor white M2R. The RCLS polysaccharides bound wheat germ agglutinin and appeared as long and thin or short and thick fibers. Accordingly, Mg2+, Mn2+, and Co2+ stimulated E. histolytica to synthesize a chitin-li]ke material.
Original languageEnglish
Pages (from-to)919-923
Number of pages5
JournalJournal of Parasitology
Publication statusPublished - 1 Jan 2001
Externally publishedYes

Fingerprint

Entamoeba histolytica
Chitin
chitin
Trophozoites
trophozoites
polysaccharide
N-acetylglucosamine
Acetylglucosamine
glucose
Polysaccharides
polysaccharides
Chitin Synthase
lysis
chitin synthase
cyst
Glucose
Chitinases
Wheat Germ Agglutinins
wheat germ
liquid chromatography

Cite this

Said-Fernández, S., Campos-Góngora, E., González-Salazar, F., Martinez-Rodriguez, H. G., Vargas-Villarreal, J., & Viader-Salvadó, J. M. (2001). Mg2+, Mn2+, and Co2+ stimulate Entamoeba histolytica to produce chitin-like material. Journal of Parasitology, 919-923.
Said-Fernández, S. ; Campos-Góngora, E. ; González-Salazar, F. ; Martinez-Rodriguez, H. G. ; Vargas-Villarreal, J. ; Viader-Salvadó, J. M. / Mg2+, Mn2+, and Co2+ stimulate Entamoeba histolytica to produce chitin-like material. In: Journal of Parasitology. 2001 ; pp. 919-923.
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abstract = "The mechanism of Entamoeba histolytica cyst cell wall synthesis is not well understood. Previous research has shown that cystlike structures formed in the presence of chitin synthase cofactors (Mg2+, Mn2+, and Co2+) resist 1{\%} sodium dodecyl sulfate lysis (RCLS), whereas those formed in the absence of cofactors (CLS) do not, and trophozoites are immediately destroyed. This suggests that E. histolytica is able to synthesize chitin, initiating a differentiation process under axenic conditions. To test this hypothesis, polysaccharide hydrolysates from E. histolytica trophozoites, CLS, or RCLS were analyzed with high-performance liquid chromatography. The major components found in all 3 preparations were N-acetylglucosamine (NAG) and glucose (GLC), with RCLS possessing 129 and 180 times more NAG and 2.4 and 2.0 more GLC than trophozoites and CLS, respectively. After 36 hr of incubation with chitinase (16 U/ml) in a hypotonic medium (50 mOsm/kg), 68{\%} of RCLS was lysed, and 100{\%} lost affinity for calcofluor white M2R. The RCLS polysaccharides bound wheat germ agglutinin and appeared as long and thin or short and thick fibers. Accordingly, Mg2+, Mn2+, and Co2+ stimulated E. histolytica to synthesize a chitin-li]ke material.",
author = "S. Said-Fern{\'a}ndez and E. Campos-G{\'o}ngora and F. Gonz{\'a}lez-Salazar and Martinez-Rodriguez, {H. G.} and J. Vargas-Villarreal and Viader-Salvad{\'o}, {J. M.}",
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Said-Fernández, S, Campos-Góngora, E, González-Salazar, F, Martinez-Rodriguez, HG, Vargas-Villarreal, J & Viader-Salvadó, JM 2001, 'Mg2+, Mn2+, and Co2+ stimulate Entamoeba histolytica to produce chitin-like material', Journal of Parasitology, pp. 919-923.

Mg2+, Mn2+, and Co2+ stimulate Entamoeba histolytica to produce chitin-like material. / Said-Fernández, S.; Campos-Góngora, E.; González-Salazar, F.; Martinez-Rodriguez, H. G.; Vargas-Villarreal, J.; Viader-Salvadó, J. M.

In: Journal of Parasitology, 01.01.2001, p. 919-923.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mg2+, Mn2+, and Co2+ stimulate Entamoeba histolytica to produce chitin-like material

AU - Said-Fernández, S.

AU - Campos-Góngora, E.

AU - González-Salazar, F.

AU - Martinez-Rodriguez, H. G.

AU - Vargas-Villarreal, J.

AU - Viader-Salvadó, J. M.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - The mechanism of Entamoeba histolytica cyst cell wall synthesis is not well understood. Previous research has shown that cystlike structures formed in the presence of chitin synthase cofactors (Mg2+, Mn2+, and Co2+) resist 1% sodium dodecyl sulfate lysis (RCLS), whereas those formed in the absence of cofactors (CLS) do not, and trophozoites are immediately destroyed. This suggests that E. histolytica is able to synthesize chitin, initiating a differentiation process under axenic conditions. To test this hypothesis, polysaccharide hydrolysates from E. histolytica trophozoites, CLS, or RCLS were analyzed with high-performance liquid chromatography. The major components found in all 3 preparations were N-acetylglucosamine (NAG) and glucose (GLC), with RCLS possessing 129 and 180 times more NAG and 2.4 and 2.0 more GLC than trophozoites and CLS, respectively. After 36 hr of incubation with chitinase (16 U/ml) in a hypotonic medium (50 mOsm/kg), 68% of RCLS was lysed, and 100% lost affinity for calcofluor white M2R. The RCLS polysaccharides bound wheat germ agglutinin and appeared as long and thin or short and thick fibers. Accordingly, Mg2+, Mn2+, and Co2+ stimulated E. histolytica to synthesize a chitin-li]ke material.

AB - The mechanism of Entamoeba histolytica cyst cell wall synthesis is not well understood. Previous research has shown that cystlike structures formed in the presence of chitin synthase cofactors (Mg2+, Mn2+, and Co2+) resist 1% sodium dodecyl sulfate lysis (RCLS), whereas those formed in the absence of cofactors (CLS) do not, and trophozoites are immediately destroyed. This suggests that E. histolytica is able to synthesize chitin, initiating a differentiation process under axenic conditions. To test this hypothesis, polysaccharide hydrolysates from E. histolytica trophozoites, CLS, or RCLS were analyzed with high-performance liquid chromatography. The major components found in all 3 preparations were N-acetylglucosamine (NAG) and glucose (GLC), with RCLS possessing 129 and 180 times more NAG and 2.4 and 2.0 more GLC than trophozoites and CLS, respectively. After 36 hr of incubation with chitinase (16 U/ml) in a hypotonic medium (50 mOsm/kg), 68% of RCLS was lysed, and 100% lost affinity for calcofluor white M2R. The RCLS polysaccharides bound wheat germ agglutinin and appeared as long and thin or short and thick fibers. Accordingly, Mg2+, Mn2+, and Co2+ stimulated E. histolytica to synthesize a chitin-li]ke material.

M3 - Article

SP - 919

EP - 923

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

ER -

Said-Fernández S, Campos-Góngora E, González-Salazar F, Martinez-Rodriguez HG, Vargas-Villarreal J, Viader-Salvadó JM. Mg2+, Mn2+, and Co2+ stimulate Entamoeba histolytica to produce chitin-like material. Journal of Parasitology. 2001 Jan 1;919-923.