Laccase-based catalytic microreactor for BPA biotransformation

Juan Eduardo Sosa-Hernández*, Elsa M. Gutierrez, Jhosseph S. Ochoa Sierra, Osvaldo Aquines Gutiérrez, Felipe Robledo-Padilla, Elda M. Melchor-Martínez, Hafiz M.N. Iqbal, Roberto Parra-Salvídar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A laccase-based catalytic reactor was developed into a polydimethylsiloxane (PDMS) microfluidic device, allowing the degradation of different concentrations of the emergent pollutant, Bisphenol-A (BPA), at a rate similar to free enzyme. Among the immobilizing agents used, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was capable of immobilizing a more significant amount of the laccase enzyme in comparison to glutaraldehyde (GA), and the passive method (2989, 1537, and 1905 U/mL, respectively). The immobilized enzyme inside the microfluidic device could degrade 55 ppm of BPA at a reaction rate of 0.5309 U/mL*min with a contaminant initial concentration of 100 ppm at room temperature. In conclusion, the design of a microfluidic device and the immobilization of the laccase enzyme successfully allowed a high capacity of BPA degradation.

Original languageEnglish
Article numbere24483
Pages (from-to)e24483
JournalHeliyon
Volume10
Issue number2
DOIs
Publication statusPublished - 30 Jan 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'Laccase-based catalytic microreactor for BPA biotransformation'. Together they form a unique fingerprint.

Cite this