Laccase-based catalytic microreactor for BPA biotransformation

Juan Eduardo Sosa-Hernández; Elsa M. Gutierrez; Jhosseph S. Ochoa Sierra; Osvaldo Aquines Gutiérrez; Felipe Robledo-Padilla; Elda M. Melchor-Martínez; Hafiz M.N. Iqbal; Roberto Parra-Salvídar

doi:10.1016/j.heliyon.2024.e24483

Laccase-based catalytic microreactor for BPA biotransformation

Juan Eduardo Sosa-Hernández^*, Elsa M. Gutierrez, Jhosseph S. Ochoa Sierra, Osvaldo Aquines Gutiérrez, Felipe Robledo-Padilla, Elda M. Melchor-Martínez, Hafiz M.N. Iqbal, Roberto Parra-Salvídar^*

^*Corresponding author for this work

Departamento de Física y Matemáticas

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A laccase-based catalytic reactor was developed into a polydimethylsiloxane (PDMS) microfluidic device, allowing the degradation of different concentrations of the emergent pollutant, Bisphenol-A (BPA), at a rate similar to free enzyme. Among the immobilizing agents used, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was capable of immobilizing a more significant amount of the laccase enzyme in comparison to glutaraldehyde (GA), and the passive method (2989, 1537, and 1905 U/mL, respectively). The immobilized enzyme inside the microfluidic device could degrade 55 ppm of BPA at a reaction rate of 0.5309 U/mL*min with a contaminant initial concentration of 100 ppm at room temperature. In conclusion, the design of a microfluidic device and the immobilization of the laccase enzyme successfully allowed a high capacity of BPA degradation.

Original language	English
Article number	e24483
Pages (from-to)	e24483
Journal	Heliyon
Volume	10
Issue number	2
DOIs	https://doi.org/10.1016/j.heliyon.2024.e24483
Publication status	Published - 30 Jan 2024

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title = "Laccase-based catalytic microreactor for BPA biotransformation",

abstract = "A laccase-based catalytic reactor was developed into a polydimethylsiloxane (PDMS) microfluidic device, allowing the degradation of different concentrations of the emergent pollutant, Bisphenol-A (BPA), at a rate similar to free enzyme. Among the immobilizing agents used, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was capable of immobilizing a more significant amount of the laccase enzyme in comparison to glutaraldehyde (GA), and the passive method (2989, 1537, and 1905 U/mL, respectively). The immobilized enzyme inside the microfluidic device could degrade 55 ppm of BPA at a reaction rate of 0.5309 U/mL*min with a contaminant initial concentration of 100 ppm at room temperature. In conclusion, the design of a microfluidic device and the immobilization of the laccase enzyme successfully allowed a high capacity of BPA degradation.",

author = "Sosa-Hern{\'a}ndez, {Juan Eduardo} and Gutierrez, {Elsa M.} and {Ochoa Sierra}, {Jhosseph S.} and {Aquines Guti{\'e}rrez}, Osvaldo and Felipe Robledo-Padilla and Melchor-Mart{\'i}nez, {Elda M.} and Iqbal, {Hafiz M.N.} and Roberto Parra-Salv{\'i}dar",

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doi = "10.1016/j.heliyon.2024.e24483",

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AU - Sosa-Hernández, Juan Eduardo

AU - Gutierrez, Elsa M.

AU - Ochoa Sierra, Jhosseph S.

AU - Aquines Gutiérrez, Osvaldo

AU - Robledo-Padilla, Felipe

AU - Melchor-Martínez, Elda M.

AU - Iqbal, Hafiz M.N.

AU - Parra-Salvídar, Roberto

PY - 2024/1/30

Y1 - 2024/1/30

N2 - A laccase-based catalytic reactor was developed into a polydimethylsiloxane (PDMS) microfluidic device, allowing the degradation of different concentrations of the emergent pollutant, Bisphenol-A (BPA), at a rate similar to free enzyme. Among the immobilizing agents used, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was capable of immobilizing a more significant amount of the laccase enzyme in comparison to glutaraldehyde (GA), and the passive method (2989, 1537, and 1905 U/mL, respectively). The immobilized enzyme inside the microfluidic device could degrade 55 ppm of BPA at a reaction rate of 0.5309 U/mL*min with a contaminant initial concentration of 100 ppm at room temperature. In conclusion, the design of a microfluidic device and the immobilization of the laccase enzyme successfully allowed a high capacity of BPA degradation.

AB - A laccase-based catalytic reactor was developed into a polydimethylsiloxane (PDMS) microfluidic device, allowing the degradation of different concentrations of the emergent pollutant, Bisphenol-A (BPA), at a rate similar to free enzyme. Among the immobilizing agents used, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was capable of immobilizing a more significant amount of the laccase enzyme in comparison to glutaraldehyde (GA), and the passive method (2989, 1537, and 1905 U/mL, respectively). The immobilized enzyme inside the microfluidic device could degrade 55 ppm of BPA at a reaction rate of 0.5309 U/mL*min with a contaminant initial concentration of 100 ppm at room temperature. In conclusion, the design of a microfluidic device and the immobilization of the laccase enzyme successfully allowed a high capacity of BPA degradation.

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Laccase-based catalytic microreactor for BPA biotransformation

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