Internal ribosome entry site (IRES) from Encephalomyocarditis virus (EMCV) as a tool for shuttle expression plasmids

Sandra Aurora Telpalo-Carpio, Francisco Diaz-Mitoma, Jorge Eugenio Moreno-Cuevas, José Manuel Aguilar-Yáñez

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In eukaryotes, IRES sequences aid the recruitment of factors needed for translation to occur, enabling protein production independent of 5′ capped mRNA. Many patents and commercially available plasmids exploit their properties for polycistronic expression of recombinant proteins. However, these applications have been restricted to eukaryotic organisms, since it was thought that elements of this origin were essential for their activity. Here, using two tricistronic vectors designed for expression in mammalian hosts, we present evidence of EMCV IRES activity in prokaryotes. This finding enables the development of new and more versatile plasmid vectors for the production of recombinant proteins in multiple hosts from a single construct. Additionally, it provides new hints for the elaboration of alternative models describing the molecular mechanism of EMCV IRES mediated translation, in the absence of eukaryotic elements that were considered indispensable for its function.

Original languageEnglish
Pages (from-to)548-553
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume468
Issue number4
DOIs
Publication statusPublished - 25 Dec 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Internal ribosome entry site (IRES) from Encephalomyocarditis virus (EMCV) as a tool for shuttle expression plasmids'. Together they form a unique fingerprint.

Cite this