In vitro antiamoebic activity of cnestis ferruginea and dianthus basuticus on entamoeba histolytica trophozoites

Teslim O. Ojuromi; Tom A.O. Ashafa; Jesus N. Garza-Gonzalez; Francisco Gonzalez-Salazar

doi:10.26538/tjnpr/v5i3.22

In vitro antiamoebic activity of cnestis ferruginea and dianthus basuticus on entamoeba histolytica trophozoites

Teslim O. Ojuromi, Tom A.O. Ashafa, Jesus N. Garza-Gonzalez, Francisco Gonzalez-Salazar^*

^*Corresponding author for this work

Departamento de Ciencias Básicas

Research output: Contribution to journal › Article › peer-review

Abstract

Amoebiasis is still a great disease burden in developing countries; this may cause around 100,000 deaths every year worldwide. The appearance of the strains of parasites resistant to conventional drugs used to treat this disease compels the search for new antiparasitic agents. As a result, a growing emphasis has been placed on plants used in traditional medicine to discover new agents with antiparasitic activity. The study determined the antiamoebic activity of Cnestis ferruginea and Dianthus basuticus found in Nigeria and South Africa. The assays were performed on cultures of Entamoeba histolytica growing in the logarithmic phase that were exposed to various concentrations of the ethanol extract of D. basuticus and C. ferruginea plants. The probit of the cultures were calculated, and the IC₅₀ was determined for each extract for antiamoebic activity. The ethanol extract of both C. ferruginea and D. basuticus demonstrated a remarkable in-vitro inhibitory activity against E. histolytica trophozoites. The 50% inhibitory concentrations (IC₅₀) of C. ferruginea and D. basuticus on E. histolytica cultures were 15.07 µg/mL and 12.45 µg/mL, respectively. The results showed that D. basuticus and C. ferruginea have inhibitory activity against Entamoeba histolytica.

Original language	English
Pages (from-to)	555-558
Number of pages	4
Journal	Tropical Journal of Natural Product Research
Volume	5
Issue number	3
DOIs	https://doi.org/10.26538/tjnpr/v5i3.22
Publication status	Published - 2021

Bibliographical note

Funding Information:
The authors thank the Directorate of Research Development University of the Free State for Postdoctoral Fellowship of Dr. Ojuromi. The authors also acknowledge the support from the management of Lagos State University, Ojo, Lagos, Nigeria. Authors acknowledge the University of Monterrey, Mexico for the support to publish the manuscript.

Publisher Copyright:
© 2021 Teslim et al.

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine

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10.26538/tjnpr/v5i3.22

Cite this

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title = "In vitro antiamoebic activity of cnestis ferruginea and dianthus basuticus on entamoeba histolytica trophozoites",

abstract = "Amoebiasis is still a great disease burden in developing countries; this may cause around 100,000 deaths every year worldwide. The appearance of the strains of parasites resistant to conventional drugs used to treat this disease compels the search for new antiparasitic agents. As a result, a growing emphasis has been placed on plants used in traditional medicine to discover new agents with antiparasitic activity. The study determined the antiamoebic activity of Cnestis ferruginea and Dianthus basuticus found in Nigeria and South Africa. The assays were performed on cultures of Entamoeba histolytica growing in the logarithmic phase that were exposed to various concentrations of the ethanol extract of D. basuticus and C. ferruginea plants. The probit of the cultures were calculated, and the IC50 was determined for each extract for antiamoebic activity. The ethanol extract of both C. ferruginea and D. basuticus demonstrated a remarkable in-vitro inhibitory activity against E. histolytica trophozoites. The 50% inhibitory concentrations (IC50) of C. ferruginea and D. basuticus on E. histolytica cultures were 15.07 µg/mL and 12.45 µg/mL, respectively. The results showed that D. basuticus and C. ferruginea have inhibitory activity against Entamoeba histolytica.",

author = "Ojuromi, {Teslim O.} and Ashafa, {Tom A.O.} and Garza-Gonzalez, {Jesus N.} and Francisco Gonzalez-Salazar",

note = "Funding Information: The authors thank the Directorate of Research Development University of the Free State for Postdoctoral Fellowship of Dr. Ojuromi. The authors also acknowledge the support from the management of Lagos State University, Ojo, Lagos, Nigeria. Authors acknowledge the University of Monterrey, Mexico for the support to publish the manuscript. Publisher Copyright: {\textcopyright} 2021 Teslim et al.",

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doi = "10.26538/tjnpr/v5i3.22",

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AU - Ashafa, Tom A.O.

AU - Garza-Gonzalez, Jesus N.

AU - Gonzalez-Salazar, Francisco

N1 - Funding Information: The authors thank the Directorate of Research Development University of the Free State for Postdoctoral Fellowship of Dr. Ojuromi. The authors also acknowledge the support from the management of Lagos State University, Ojo, Lagos, Nigeria. Authors acknowledge the University of Monterrey, Mexico for the support to publish the manuscript. Publisher Copyright: © 2021 Teslim et al.

PY - 2021

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AB - Amoebiasis is still a great disease burden in developing countries; this may cause around 100,000 deaths every year worldwide. The appearance of the strains of parasites resistant to conventional drugs used to treat this disease compels the search for new antiparasitic agents. As a result, a growing emphasis has been placed on plants used in traditional medicine to discover new agents with antiparasitic activity. The study determined the antiamoebic activity of Cnestis ferruginea and Dianthus basuticus found in Nigeria and South Africa. The assays were performed on cultures of Entamoeba histolytica growing in the logarithmic phase that were exposed to various concentrations of the ethanol extract of D. basuticus and C. ferruginea plants. The probit of the cultures were calculated, and the IC50 was determined for each extract for antiamoebic activity. The ethanol extract of both C. ferruginea and D. basuticus demonstrated a remarkable in-vitro inhibitory activity against E. histolytica trophozoites. The 50% inhibitory concentrations (IC50) of C. ferruginea and D. basuticus on E. histolytica cultures were 15.07 µg/mL and 12.45 µg/mL, respectively. The results showed that D. basuticus and C. ferruginea have inhibitory activity against Entamoeba histolytica.

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In vitro antiamoebic activity of cnestis ferruginea and dianthus basuticus on entamoeba histolytica trophozoites

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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