In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana

Zhelmy Martín-Quintal; Rosa Moo-Puc; Francisco González-Salazar; Manuel J. Chan-Bacab; Luis W. Torres-Tapia; Sergio R. Peraza-Sánchez

doi:10.1016/j.jep.2009.01.037

In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana

Zhelmy Martín-Quintal, Rosa Moo-Puc, Francisco González-Salazar, Manuel J. Chan-Bacab, Luis W. Torres-Tapia, Sergio R. Peraza-Sánchez

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41 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Tridax procumbens is an active herb against leishmaniasis. Aim of the study: Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World. Materials and methods: Extracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT. Results: We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50% inhibitory concentration (IC ₅₀) of 3 μg/ml, while oxylipin 1 exhibited the highest inhibition at IC ₅₀ = 0.478 μg/ml. Conclusions: In this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells.

Original language	English
Pages (from-to)	463-467
Number of pages	5
Journal	Journal of Ethnopharmacology
Volume	122
Issue number	3
DOIs	https://doi.org/10.1016/j.jep.2009.01.037
Publication status	Published - 21 Apr 2009
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by FOMIX-Yucatan (grant #66262) and jointly by the International Foundation for Science (IFS), Stockholm, Sweden, and the Organization for the Prohibition of Chemical Weapons (OPCW), The Hague, The Netherlands, through a grant no. F/3290-2 to M.J. Chan-Bacab.

Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

Pharmacology
Drug Discovery

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Cite this

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title = "In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana",

abstract = "Ethnopharmacological relevance: Tridax procumbens is an active herb against leishmaniasis. Aim of the study: Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World. Materials and methods: Extracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT. Results: We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50% inhibitory concentration (IC 50) of 3 μg/ml, while oxylipin 1 exhibited the highest inhibition at IC 50 = 0.478 μg/ml. Conclusions: In this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells.",

author = "Zhelmy Mart{\'i}n-Quintal and Rosa Moo-Puc and Francisco Gonz{\'a}lez-Salazar and Chan-Bacab, {Manuel J.} and Torres-Tapia, {Luis W.} and Peraza-S{\'a}nchez, {Sergio R.}",

note = "Funding Information: This work was supported by FOMIX-Yucatan (grant #66262) and jointly by the International Foundation for Science (IFS), Stockholm, Sweden, and the Organization for the Prohibition of Chemical Weapons (OPCW), The Hague, The Netherlands, through a grant no. F/3290-2 to M.J. Chan-Bacab. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2009",

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doi = "10.1016/j.jep.2009.01.037",

language = "English",

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AU - Martín-Quintal, Zhelmy

AU - Moo-Puc, Rosa

AU - González-Salazar, Francisco

AU - Chan-Bacab, Manuel J.

AU - Torres-Tapia, Luis W.

AU - Peraza-Sánchez, Sergio R.

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PY - 2009/4/21

Y1 - 2009/4/21

N2 - Ethnopharmacological relevance: Tridax procumbens is an active herb against leishmaniasis. Aim of the study: Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World. Materials and methods: Extracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT. Results: We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50% inhibitory concentration (IC 50) of 3 μg/ml, while oxylipin 1 exhibited the highest inhibition at IC 50 = 0.478 μg/ml. Conclusions: In this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells.

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In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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