In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana

Zhelmy Martín-Quintal, Rosa Moo-Puc, Francisco González-Salazar, Manuel J. Chan-Bacab, Luis W. Torres-Tapia, Sergio R. Peraza-Sánchez

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Tridax procumbens is an active herb against leishmaniasis. Aim of the study: Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World. Materials and methods: Extracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT. Results: We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50% inhibitory concentration (IC50) of 3 μg/ml, while oxylipin 1 exhibited the highest inhibition at IC50= 0.478 μg/ml. Conclusions: In this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells. © 2009 Elsevier Ireland Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalJournal of Ethnopharmacology
DOIs
Publication statusPublished - 21 Apr 2009
Externally publishedYes

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Leishmania mexicana
Inhibitory Concentration 50
Leishmaniasis
Oxylipins
Cutaneous Leishmaniasis
Trypan Blue
Leishmania
Growth
Ireland
Methanol
Cell Survival
In Vitro Techniques
Safety

Cite this

Martín-Quintal, Z., Moo-Puc, R., González-Salazar, F., Chan-Bacab, M. J., Torres-Tapia, L. W., & Peraza-Sánchez, S. R. (2009). In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana. Journal of Ethnopharmacology, 463-467. https://doi.org/10.1016/j.jep.2009.01.037
Martín-Quintal, Zhelmy ; Moo-Puc, Rosa ; González-Salazar, Francisco ; Chan-Bacab, Manuel J. ; Torres-Tapia, Luis W. ; Peraza-Sánchez, Sergio R. / In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana. In: Journal of Ethnopharmacology. 2009 ; pp. 463-467.
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abstract = "Ethnopharmacological relevance: Tridax procumbens is an active herb against leishmaniasis. Aim of the study: Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World. Materials and methods: Extracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT. Results: We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50{\%} inhibitory concentration (IC50) of 3 μg/ml, while oxylipin 1 exhibited the highest inhibition at IC50= 0.478 μg/ml. Conclusions: In this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells. {\circledC} 2009 Elsevier Ireland Ltd. All rights reserved.",
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In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana. / Martín-Quintal, Zhelmy; Moo-Puc, Rosa; González-Salazar, Francisco; Chan-Bacab, Manuel J.; Torres-Tapia, Luis W.; Peraza-Sánchez, Sergio R.

In: Journal of Ethnopharmacology, 21.04.2009, p. 463-467.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana

AU - Martín-Quintal, Zhelmy

AU - Moo-Puc, Rosa

AU - González-Salazar, Francisco

AU - Chan-Bacab, Manuel J.

AU - Torres-Tapia, Luis W.

AU - Peraza-Sánchez, Sergio R.

PY - 2009/4/21

Y1 - 2009/4/21

N2 - Ethnopharmacological relevance: Tridax procumbens is an active herb against leishmaniasis. Aim of the study: Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World. Materials and methods: Extracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT. Results: We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50% inhibitory concentration (IC50) of 3 μg/ml, while oxylipin 1 exhibited the highest inhibition at IC50= 0.478 μg/ml. Conclusions: In this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells. © 2009 Elsevier Ireland Ltd. All rights reserved.

AB - Ethnopharmacological relevance: Tridax procumbens is an active herb against leishmaniasis. Aim of the study: Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World. Materials and methods: Extracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT. Results: We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50% inhibitory concentration (IC50) of 3 μg/ml, while oxylipin 1 exhibited the highest inhibition at IC50= 0.478 μg/ml. Conclusions: In this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells. © 2009 Elsevier Ireland Ltd. All rights reserved.

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DO - 10.1016/j.jep.2009.01.037

M3 - Article

SP - 463

EP - 467

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

ER -