In situ detection and distribution of inflammatory cytokines during the course of infection with Nocardia brasiliensis

J. M. Solis-Soto, L. E. Quintanilla-Rodriguez, I. Meester, J. C. Segoviano-Ramirez, J. L. Vazquez-Juarez, M. C. Salinas Carmona

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Actinomycetoma, caused by the intracellular bacterium Nocardia brasiliensis, is characterized by an infiltration of several inflammatory cell populations. To explore aspects of the immune response in the pathogenesis of these bacteria we injected 106 CFU in footpads of BALB/c mice. After 1, 2, 3, 4, 7, 30 and 90 days immunohistochemistry was performed to compare presence and distribution of the inflammatory cytokines TNF-alpha, IL-1 beta, IL-6, IFN-gamma, IL-4, IL-10, and TGF-beta. Analysis of serial paraffin tissue sections showed strong participation and differences in distribution of cytokine-producing cells during the course of infection. Several TNF-alpha immunoreactive lymphocytes of the dermis were present during the course of the infection, but absent in the site of inflammation. During the first 4 days, IL-1 beta immunoreactivity was observed in dendritic epidermal cells and in cells surrounding the neutrophils around the grain. In later stages of infection, immunoreactive cells to this cytokine were mainly in the periphery of the microabscesses. Strong immunoreactivity was observed with IL-6 during the course of infection. Some cells in the epidermis and dermis, as well as muscle cells and several cells at the periphery of the microabscesses, showed strong IL-6 immunoreactivity. Cells immunoreactive to IL-4, IL-10, IFN-gamma and TGF-beta were present at the site of infection and, in later stages, in cells at the periphery of the microabscesses. In conclusion a mix of proinflammatory and antiinflammatory cytokines are produced at the same time by host cells. According to their distribution, inflammatory cytokines seems to have different functions during the course of infection with the intracellular bacterium N. brasiliensis.
Original languageEnglish
Pages (from-to)573-581
Number of pages9
JournalHistology and Histopathology
Publication statusPublished - 1 May 2008

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Nocardia Infections
Cytokines
Infection
Interleukin-6
Dermis
Bacteria
Interleukin-1beta
Interleukin-4
Transforming Growth Factor beta
Interleukin-10
Tumor Necrosis Factor-alpha
Mycetoma
Nocardia
Langerhans Cells
Epidermis
Paraffin
Muscle Cells
Neutrophils
Anti-Inflammatory Agents
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

Cite this

Solis-Soto, J. M., Quintanilla-Rodriguez, L. E., Meester, I., Segoviano-Ramirez, J. C., Vazquez-Juarez, J. L., & Salinas Carmona, M. C. (2008). In situ detection and distribution of inflammatory cytokines during the course of infection with Nocardia brasiliensis. Histology and Histopathology, 573-581.
Solis-Soto, J. M. ; Quintanilla-Rodriguez, L. E. ; Meester, I. ; Segoviano-Ramirez, J. C. ; Vazquez-Juarez, J. L. ; Salinas Carmona, M. C. / In situ detection and distribution of inflammatory cytokines during the course of infection with Nocardia brasiliensis. In: Histology and Histopathology. 2008 ; pp. 573-581.
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abstract = "Actinomycetoma, caused by the intracellular bacterium Nocardia brasiliensis, is characterized by an infiltration of several inflammatory cell populations. To explore aspects of the immune response in the pathogenesis of these bacteria we injected 106 CFU in footpads of BALB/c mice. After 1, 2, 3, 4, 7, 30 and 90 days immunohistochemistry was performed to compare presence and distribution of the inflammatory cytokines TNF-alpha, IL-1 beta, IL-6, IFN-gamma, IL-4, IL-10, and TGF-beta. Analysis of serial paraffin tissue sections showed strong participation and differences in distribution of cytokine-producing cells during the course of infection. Several TNF-alpha immunoreactive lymphocytes of the dermis were present during the course of the infection, but absent in the site of inflammation. During the first 4 days, IL-1 beta immunoreactivity was observed in dendritic epidermal cells and in cells surrounding the neutrophils around the grain. In later stages of infection, immunoreactive cells to this cytokine were mainly in the periphery of the microabscesses. Strong immunoreactivity was observed with IL-6 during the course of infection. Some cells in the epidermis and dermis, as well as muscle cells and several cells at the periphery of the microabscesses, showed strong IL-6 immunoreactivity. Cells immunoreactive to IL-4, IL-10, IFN-gamma and TGF-beta were present at the site of infection and, in later stages, in cells at the periphery of the microabscesses. In conclusion a mix of proinflammatory and antiinflammatory cytokines are produced at the same time by host cells. According to their distribution, inflammatory cytokines seems to have different functions during the course of infection with the intracellular bacterium N. brasiliensis.",
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Solis-Soto, JM, Quintanilla-Rodriguez, LE, Meester, I, Segoviano-Ramirez, JC, Vazquez-Juarez, JL & Salinas Carmona, MC 2008, 'In situ detection and distribution of inflammatory cytokines during the course of infection with Nocardia brasiliensis', Histology and Histopathology, pp. 573-581.

In situ detection and distribution of inflammatory cytokines during the course of infection with Nocardia brasiliensis. / Solis-Soto, J. M.; Quintanilla-Rodriguez, L. E.; Meester, I.; Segoviano-Ramirez, J. C.; Vazquez-Juarez, J. L.; Salinas Carmona, M. C.

In: Histology and Histopathology, 01.05.2008, p. 573-581.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In situ detection and distribution of inflammatory cytokines during the course of infection with Nocardia brasiliensis

AU - Solis-Soto, J. M.

AU - Quintanilla-Rodriguez, L. E.

AU - Meester, I.

AU - Segoviano-Ramirez, J. C.

AU - Vazquez-Juarez, J. L.

AU - Salinas Carmona, M. C.

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Actinomycetoma, caused by the intracellular bacterium Nocardia brasiliensis, is characterized by an infiltration of several inflammatory cell populations. To explore aspects of the immune response in the pathogenesis of these bacteria we injected 106 CFU in footpads of BALB/c mice. After 1, 2, 3, 4, 7, 30 and 90 days immunohistochemistry was performed to compare presence and distribution of the inflammatory cytokines TNF-alpha, IL-1 beta, IL-6, IFN-gamma, IL-4, IL-10, and TGF-beta. Analysis of serial paraffin tissue sections showed strong participation and differences in distribution of cytokine-producing cells during the course of infection. Several TNF-alpha immunoreactive lymphocytes of the dermis were present during the course of the infection, but absent in the site of inflammation. During the first 4 days, IL-1 beta immunoreactivity was observed in dendritic epidermal cells and in cells surrounding the neutrophils around the grain. In later stages of infection, immunoreactive cells to this cytokine were mainly in the periphery of the microabscesses. Strong immunoreactivity was observed with IL-6 during the course of infection. Some cells in the epidermis and dermis, as well as muscle cells and several cells at the periphery of the microabscesses, showed strong IL-6 immunoreactivity. Cells immunoreactive to IL-4, IL-10, IFN-gamma and TGF-beta were present at the site of infection and, in later stages, in cells at the periphery of the microabscesses. In conclusion a mix of proinflammatory and antiinflammatory cytokines are produced at the same time by host cells. According to their distribution, inflammatory cytokines seems to have different functions during the course of infection with the intracellular bacterium N. brasiliensis.

AB - Actinomycetoma, caused by the intracellular bacterium Nocardia brasiliensis, is characterized by an infiltration of several inflammatory cell populations. To explore aspects of the immune response in the pathogenesis of these bacteria we injected 106 CFU in footpads of BALB/c mice. After 1, 2, 3, 4, 7, 30 and 90 days immunohistochemistry was performed to compare presence and distribution of the inflammatory cytokines TNF-alpha, IL-1 beta, IL-6, IFN-gamma, IL-4, IL-10, and TGF-beta. Analysis of serial paraffin tissue sections showed strong participation and differences in distribution of cytokine-producing cells during the course of infection. Several TNF-alpha immunoreactive lymphocytes of the dermis were present during the course of the infection, but absent in the site of inflammation. During the first 4 days, IL-1 beta immunoreactivity was observed in dendritic epidermal cells and in cells surrounding the neutrophils around the grain. In later stages of infection, immunoreactive cells to this cytokine were mainly in the periphery of the microabscesses. Strong immunoreactivity was observed with IL-6 during the course of infection. Some cells in the epidermis and dermis, as well as muscle cells and several cells at the periphery of the microabscesses, showed strong IL-6 immunoreactivity. Cells immunoreactive to IL-4, IL-10, IFN-gamma and TGF-beta were present at the site of infection and, in later stages, in cells at the periphery of the microabscesses. In conclusion a mix of proinflammatory and antiinflammatory cytokines are produced at the same time by host cells. According to their distribution, inflammatory cytokines seems to have different functions during the course of infection with the intracellular bacterium N. brasiliensis.

M3 - Article

SP - 573

EP - 581

JO - Histology and Histopathology

JF - Histology and Histopathology

SN - 0213-3911

ER -

Solis-Soto JM, Quintanilla-Rodriguez LE, Meester I, Segoviano-Ramirez JC, Vazquez-Juarez JL, Salinas Carmona MC. In situ detection and distribution of inflammatory cytokines during the course of infection with Nocardia brasiliensis. Histology and Histopathology. 2008 May 1;573-581.