Immune Response to Nocardia brasiliensisAntigens in an Experimental Model of Actinomycetoma in BALB/c Mice

Ernesto Torres López, MARIO CESAR SALINAS-CARMONA, ALMA I RAMOS-CANO, ANGEL LICON-TRILLO, DANIEL GONZALÉZ-SPENCER

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Abstract

Nine- to twelve-week-old BALB/c mice were injected in footpads with 107 CFU of a Nocardia brasiliensis cell
suspension. Typical actinomycetoma lesions, characterized by severe local inflammation with abscess and
fistula formation, were fully established by day 28 after infection. These changes presented for 90 days, and
then tissue repair with scar formation slowly appeared, with complete healing after 150 days of infection. Some
animals developed bone destruction in the affected area. Histopathology showed an intense inflammatory
response, with polymorphonuclear cells and hyaloid material around the colonies of the bacteria, some of
which were discharged from draining abscesses. Sera from experimental animals were analyzed by Western
blotting, and immunodominant antigens P61 and P24 were found as major targets for antibody response.
Anti-P24 immunoglobulin M (IgM) isotype antibodies were present as early as 7 days, IgG peaking 45 days
after infection. Lymphocyte proliferation with spleen and popliteal lymph node cells demonstrated thymidine
incorporation at 7 days after infection, the stimulation index decreasing by day 60. Levels of interleukin-1
(IL-1), IL-2, IL-4, IL-6, tumor necrosis factor alpha, and gamma interferon (IFN-g) were determined by
enzyme-linked immunosorbent assay in the sera of infected animals. The circulating levels of IFN-g increased
more than 10 times the basal levels; levels of IL-4, IL-6 and IL-10 also increased during the first 4 days of
infection.
Original languageEnglish
Pages (from-to)2428
Number of pages2432
JournalInfection and Immunity
Volume67
Issue number5
Early online dateMay 1999
Publication statusPublished - May 1999

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Mycetoma
Nocardia
Theoretical Models
Infection
Interleukin-4
Abscess
Interleukin-6
Immunodominant Epitopes
Immunosorbents
Immunoglobulin Isotypes
Serum
Interleukin-1
Interferon-alpha
Interleukin-10
Interferon-gamma
Antibody Formation
Interleukin-2
Cicatrix
Immunoglobulin M
Spleen

Cite this

Torres López, E., SALINAS-CARMONA, MARIO. CESAR., RAMOS-CANO, ALMA. I., LICON-TRILLO, ANGEL., & GONZALÉZ-SPENCER, DANIEL. (1999). Immune Response to Nocardia brasiliensisAntigens in an Experimental Model of Actinomycetoma in BALB/c Mice. Infection and Immunity, 67(5), 2428.
Torres López, Ernesto ; SALINAS-CARMONA, MARIO CESAR ; RAMOS-CANO, ALMA I ; LICON-TRILLO, ANGEL ; GONZALÉZ-SPENCER, DANIEL . / Immune Response to Nocardia brasiliensisAntigens in an Experimental Model of Actinomycetoma in BALB/c Mice. In: Infection and Immunity. 1999 ; Vol. 67, No. 5. pp. 2428.
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abstract = "Nine- to twelve-week-old BALB/c mice were injected in footpads with 107 CFU of a Nocardia brasiliensis cellsuspension. Typical actinomycetoma lesions, characterized by severe local inflammation with abscess andfistula formation, were fully established by day 28 after infection. These changes presented for 90 days, andthen tissue repair with scar formation slowly appeared, with complete healing after 150 days of infection. Someanimals developed bone destruction in the affected area. Histopathology showed an intense inflammatoryresponse, with polymorphonuclear cells and hyaloid material around the colonies of the bacteria, some ofwhich were discharged from draining abscesses. Sera from experimental animals were analyzed by Westernblotting, and immunodominant antigens P61 and P24 were found as major targets for antibody response.Anti-P24 immunoglobulin M (IgM) isotype antibodies were present as early as 7 days, IgG peaking 45 daysafter infection. Lymphocyte proliferation with spleen and popliteal lymph node cells demonstrated thymidineincorporation at 7 days after infection, the stimulation index decreasing by day 60. Levels of interleukin-1(IL-1), IL-2, IL-4, IL-6, tumor necrosis factor alpha, and gamma interferon (IFN-g) were determined byenzyme-linked immunosorbent assay in the sera of infected animals. The circulating levels of IFN-g increasedmore than 10 times the basal levels; levels of IL-4, IL-6 and IL-10 also increased during the first 4 days ofinfection.",
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Torres López, E, SALINAS-CARMONA, MARIOCESAR, RAMOS-CANO, ALMAI, LICON-TRILLO, ANGEL & GONZALÉZ-SPENCER, DANIEL 1999, 'Immune Response to Nocardia brasiliensisAntigens in an Experimental Model of Actinomycetoma in BALB/c Mice', Infection and Immunity, vol. 67, no. 5, pp. 2428.

Immune Response to Nocardia brasiliensisAntigens in an Experimental Model of Actinomycetoma in BALB/c Mice. / Torres López, Ernesto; SALINAS-CARMONA, MARIO CESAR; RAMOS-CANO, ALMA I; LICON-TRILLO, ANGEL ; GONZALÉZ-SPENCER, DANIEL .

In: Infection and Immunity, Vol. 67, No. 5, 05.1999, p. 2428.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immune Response to Nocardia brasiliensisAntigens in an Experimental Model of Actinomycetoma in BALB/c Mice

AU - Torres López, Ernesto

AU - SALINAS-CARMONA, MARIO CESAR

AU - RAMOS-CANO, ALMA I

AU - LICON-TRILLO, ANGEL

AU - GONZALÉZ-SPENCER, DANIEL

PY - 1999/5

Y1 - 1999/5

N2 - Nine- to twelve-week-old BALB/c mice were injected in footpads with 107 CFU of a Nocardia brasiliensis cellsuspension. Typical actinomycetoma lesions, characterized by severe local inflammation with abscess andfistula formation, were fully established by day 28 after infection. These changes presented for 90 days, andthen tissue repair with scar formation slowly appeared, with complete healing after 150 days of infection. Someanimals developed bone destruction in the affected area. Histopathology showed an intense inflammatoryresponse, with polymorphonuclear cells and hyaloid material around the colonies of the bacteria, some ofwhich were discharged from draining abscesses. Sera from experimental animals were analyzed by Westernblotting, and immunodominant antigens P61 and P24 were found as major targets for antibody response.Anti-P24 immunoglobulin M (IgM) isotype antibodies were present as early as 7 days, IgG peaking 45 daysafter infection. Lymphocyte proliferation with spleen and popliteal lymph node cells demonstrated thymidineincorporation at 7 days after infection, the stimulation index decreasing by day 60. Levels of interleukin-1(IL-1), IL-2, IL-4, IL-6, tumor necrosis factor alpha, and gamma interferon (IFN-g) were determined byenzyme-linked immunosorbent assay in the sera of infected animals. The circulating levels of IFN-g increasedmore than 10 times the basal levels; levels of IL-4, IL-6 and IL-10 also increased during the first 4 days ofinfection.

AB - Nine- to twelve-week-old BALB/c mice were injected in footpads with 107 CFU of a Nocardia brasiliensis cellsuspension. Typical actinomycetoma lesions, characterized by severe local inflammation with abscess andfistula formation, were fully established by day 28 after infection. These changes presented for 90 days, andthen tissue repair with scar formation slowly appeared, with complete healing after 150 days of infection. Someanimals developed bone destruction in the affected area. Histopathology showed an intense inflammatoryresponse, with polymorphonuclear cells and hyaloid material around the colonies of the bacteria, some ofwhich were discharged from draining abscesses. Sera from experimental animals were analyzed by Westernblotting, and immunodominant antigens P61 and P24 were found as major targets for antibody response.Anti-P24 immunoglobulin M (IgM) isotype antibodies were present as early as 7 days, IgG peaking 45 daysafter infection. Lymphocyte proliferation with spleen and popliteal lymph node cells demonstrated thymidineincorporation at 7 days after infection, the stimulation index decreasing by day 60. Levels of interleukin-1(IL-1), IL-2, IL-4, IL-6, tumor necrosis factor alpha, and gamma interferon (IFN-g) were determined byenzyme-linked immunosorbent assay in the sera of infected animals. The circulating levels of IFN-g increasedmore than 10 times the basal levels; levels of IL-4, IL-6 and IL-10 also increased during the first 4 days ofinfection.

M3 - Article

VL - 67

SP - 2428

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 5

ER -

Torres López E, SALINAS-CARMONA MARIOCESAR, RAMOS-CANO ALMAI, LICON-TRILLO ANGEL, GONZALÉZ-SPENCER DANIEL. Immune Response to Nocardia brasiliensisAntigens in an Experimental Model of Actinomycetoma in BALB/c Mice. Infection and Immunity. 1999 May;67(5):2428.