TY - JOUR
T1 - Human hematopoietic progenitor cells grow faster under rotational laminar flows.
AU - Murugappan, G.
AU - Carrillo-Cocom, L. M.
AU - Johnson, K. E.
AU - González-Barrón, M. T.
AU - Moreno-Cuevas, J. E.
AU - Alvarez, M. M.
N1 - © 2010 American Institute of Chemical Engineers
PY - 2010/9
Y1 - 2010/9
N2 - We report significant and reproducible growth acceleration of human progenitor cells when exposed to rotational flow when compared with stationary conditions. Nonenriched CD34+ umbilical cord derived human hematopoietic progenitor cells were cultured in Petri dishes located at different radial distances with respect to the central axis of a rotating platform. Growth dynamics under 3 or 5 rpm agitation was compared against that observed under typical stationary conditions. Cells cultured at 3 or 5 rpm exhibited (a) the absence of a latency phase, (b) an increase in final cell concentrations by 54-58.5%, and (c) reduced doubling time in their exponential phase by 12-16% in comparison with stationary culture. Cells grown under rotational agitation were confirmed to remain CD34+ by PCR. These results document a significant positive effect of exposure to laminar flow fields on the growth of human hematopoietic progenitor cells.
AB - We report significant and reproducible growth acceleration of human progenitor cells when exposed to rotational flow when compared with stationary conditions. Nonenriched CD34+ umbilical cord derived human hematopoietic progenitor cells were cultured in Petri dishes located at different radial distances with respect to the central axis of a rotating platform. Growth dynamics under 3 or 5 rpm agitation was compared against that observed under typical stationary conditions. Cells cultured at 3 or 5 rpm exhibited (a) the absence of a latency phase, (b) an increase in final cell concentrations by 54-58.5%, and (c) reduced doubling time in their exponential phase by 12-16% in comparison with stationary culture. Cells grown under rotational agitation were confirmed to remain CD34+ by PCR. These results document a significant positive effect of exposure to laminar flow fields on the growth of human hematopoietic progenitor cells.
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U2 - 10.1002/btpr.440
DO - 10.1002/btpr.440
M3 - Article
C2 - 20665657
AN - SCOPUS:79952199952
SN - 8756-7938
VL - 26
SP - 1465
EP - 1473
JO - Biotechnology Progress
JF - Biotechnology Progress
IS - 5
ER -