Human hematopoietic progenitor cells grow faster under rotational laminar flows.

G. Murugappan, L. M. Carrillo-Cocom, K. E. Johnson, M. T. González-Barrón, J. E. Moreno-Cuevas, M. M. Alvarez

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

We report significant and reproducible growth acceleration of human progenitor cells when exposed to rotational flow when compared with stationary conditions. Nonenriched CD34+ umbilical cord derived human hematopoietic progenitor cells were cultured in Petri dishes located at different radial distances with respect to the central axis of a rotating platform. Growth dynamics under 3 or 5 rpm agitation was compared against that observed under typical stationary conditions. Cells cultured at 3 or 5 rpm exhibited (a) the absence of a latency phase, (b) an increase in final cell concentrations by 54-58.5%, and (c) reduced doubling time in their exponential phase by 12-16% in comparison with stationary culture. Cells grown under rotational agitation were confirmed to remain CD34+ by PCR. These results document a significant positive effect of exposure to laminar flow fields on the growth of human hematopoietic progenitor cells.

Original languageEnglish
Pages (from-to)1465-1473
Number of pages9
JournalBiotechnology Progress
Volume26
Issue number5
DOIs
Publication statusPublished - Sep 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology

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