Hepatoprotective effect of commercial herbal extracts on carbon tetrachloride-induced liver damage in Wistar rats

Paula Cordero-Pérez, Liliana Torres-González, Marcelino Aguirre-Garza, Carlos Camara-Lemarroy, Francisco Guzmán-De La Garza, Gabriela Alarcón-Galván, Homero Zapata-Chavira, Ma De Jesús Sotelo-Gallegos, Cipactli Nadjedja Torres-Esquivel, Ethel Sánchez-Fresno, Daniel Cantú-Sepúlveda, Gerardo González-Saldivar, Judith Bernal-Ramirez, Linda E. Muñoz-Espinosa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background : Various hepatoprotective herbal products from plants are available in Mexico, where up to 85% of patients with liver disease use some form of complementary and alternative medicine. However, only few studies have reported on the biological evaluation of these products. Objective : Using a model of carbon tetrachloride (CCl4)-induced hepatotoxicity in rats, we evaluated the effects of commercial herbal extracts used most commonly in the metropolitan area of Monterrey, Mexico. Materials and Methods : The commercial products were identified through surveys in public areas. The effect of these products given with or without CCl4in rats was evaluated by measuring the serum concentrations of aspartate amino transferase (AST) and alanine amino transferase (ALT), and histopathological analysis. Legalon®was used as the standard drug. Results : The most commonly used herbal products were Hepatisan®capsules, Boldo capsules, Hepavida®capsules, Boldo infusion, and milk thistle herbal supplement (80% silymarin). None of the products tested was hepatotoxic according to transaminase and histological analyses. AST and ALT activities were significantly lower in the Hepavida+CCl4-treated group as compared with the CCl4-only group. AST and ALT activities in the silymarin, Hepatisan, and Boldo tea groups were similar to those in the CCl4group. The CCl4group displayed submassive confluent necrosis and mixed inflammatory infiltration. Both the Hepatisan+CCl4and Boldo tea+CCl4groups exhibited ballooning degeneration, inflammatory infiltration, and lytic necrosis. The silymarin+CCl4group exhibited microvesicular steatosis. The Hepavida+CCl4- and Legalon+CCL4-treated groups had lower percentages of necrotic cells as compared with the CCl4-treated group; this treatment was hepatoprotective against necrosis. Conclusion : Only Hepavida had a hepatoprotective effect.
Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalPharmacognosy Research
DOIs
Publication statusPublished - 1 Jul 2013
Externally publishedYes

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Peumus
Carbon Tetrachloride
Transferases
Silymarin
Wistar Rats
Liver
Aspartic Acid
Alanine
Necrosis
Tea
Complementary Therapies
Mexico
Milk Thistle
Transaminases
Capsules
Liver Diseases
Serum
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

Cordero-Pérez, P., Torres-González, L., Aguirre-Garza, M., Camara-Lemarroy, C., Guzmán-De La Garza, F., Alarcón-Galván, G., ... Muñoz-Espinosa, L. E. (2013). Hepatoprotective effect of commercial herbal extracts on carbon tetrachloride-induced liver damage in Wistar rats. Pharmacognosy Research, 150-156. https://doi.org/10.4103/0974-8490.112417
Cordero-Pérez, Paula ; Torres-González, Liliana ; Aguirre-Garza, Marcelino ; Camara-Lemarroy, Carlos ; Guzmán-De La Garza, Francisco ; Alarcón-Galván, Gabriela ; Zapata-Chavira, Homero ; De Jesús Sotelo-Gallegos, Ma ; Torres-Esquivel, Cipactli Nadjedja ; Sánchez-Fresno, Ethel ; Cantú-Sepúlveda, Daniel ; González-Saldivar, Gerardo ; Bernal-Ramirez, Judith ; Muñoz-Espinosa, Linda E. / Hepatoprotective effect of commercial herbal extracts on carbon tetrachloride-induced liver damage in Wistar rats. In: Pharmacognosy Research. 2013 ; pp. 150-156.
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abstract = "Background : Various hepatoprotective herbal products from plants are available in Mexico, where up to 85{\%} of patients with liver disease use some form of complementary and alternative medicine. However, only few studies have reported on the biological evaluation of these products. Objective : Using a model of carbon tetrachloride (CCl4)-induced hepatotoxicity in rats, we evaluated the effects of commercial herbal extracts used most commonly in the metropolitan area of Monterrey, Mexico. Materials and Methods : The commercial products were identified through surveys in public areas. The effect of these products given with or without CCl4in rats was evaluated by measuring the serum concentrations of aspartate amino transferase (AST) and alanine amino transferase (ALT), and histopathological analysis. Legalon{\circledR}was used as the standard drug. Results : The most commonly used herbal products were Hepatisan{\circledR}capsules, Boldo capsules, Hepavida{\circledR}capsules, Boldo infusion, and milk thistle herbal supplement (80{\%} silymarin). None of the products tested was hepatotoxic according to transaminase and histological analyses. AST and ALT activities were significantly lower in the Hepavida+CCl4-treated group as compared with the CCl4-only group. AST and ALT activities in the silymarin, Hepatisan, and Boldo tea groups were similar to those in the CCl4group. The CCl4group displayed submassive confluent necrosis and mixed inflammatory infiltration. Both the Hepatisan+CCl4and Boldo tea+CCl4groups exhibited ballooning degeneration, inflammatory infiltration, and lytic necrosis. The silymarin+CCl4group exhibited microvesicular steatosis. The Hepavida+CCl4- and Legalon+CCL4-treated groups had lower percentages of necrotic cells as compared with the CCl4-treated group; this treatment was hepatoprotective against necrosis. Conclusion : Only Hepavida had a hepatoprotective effect.",
author = "Paula Cordero-P{\'e}rez and Liliana Torres-Gonz{\'a}lez and Marcelino Aguirre-Garza and Carlos Camara-Lemarroy and {Guzm{\'a}n-De La Garza}, Francisco and Gabriela Alarc{\'o}n-Galv{\'a}n and Homero Zapata-Chavira and {De Jes{\'u}s Sotelo-Gallegos}, Ma and Torres-Esquivel, {Cipactli Nadjedja} and Ethel S{\'a}nchez-Fresno and Daniel Cant{\'u}-Sep{\'u}lveda and Gerardo Gonz{\'a}lez-Saldivar and Judith Bernal-Ramirez and Mu{\~n}oz-Espinosa, {Linda E.}",
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Cordero-Pérez, P, Torres-González, L, Aguirre-Garza, M, Camara-Lemarroy, C, Guzmán-De La Garza, F, Alarcón-Galván, G, Zapata-Chavira, H, De Jesús Sotelo-Gallegos, M, Torres-Esquivel, CN, Sánchez-Fresno, E, Cantú-Sepúlveda, D, González-Saldivar, G, Bernal-Ramirez, J & Muñoz-Espinosa, LE 2013, 'Hepatoprotective effect of commercial herbal extracts on carbon tetrachloride-induced liver damage in Wistar rats', Pharmacognosy Research, pp. 150-156. https://doi.org/10.4103/0974-8490.112417

Hepatoprotective effect of commercial herbal extracts on carbon tetrachloride-induced liver damage in Wistar rats. / Cordero-Pérez, Paula; Torres-González, Liliana; Aguirre-Garza, Marcelino; Camara-Lemarroy, Carlos; Guzmán-De La Garza, Francisco; Alarcón-Galván, Gabriela; Zapata-Chavira, Homero; De Jesús Sotelo-Gallegos, Ma; Torres-Esquivel, Cipactli Nadjedja; Sánchez-Fresno, Ethel; Cantú-Sepúlveda, Daniel; González-Saldivar, Gerardo; Bernal-Ramirez, Judith; Muñoz-Espinosa, Linda E.

In: Pharmacognosy Research, 01.07.2013, p. 150-156.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hepatoprotective effect of commercial herbal extracts on carbon tetrachloride-induced liver damage in Wistar rats

AU - Cordero-Pérez, Paula

AU - Torres-González, Liliana

AU - Aguirre-Garza, Marcelino

AU - Camara-Lemarroy, Carlos

AU - Guzmán-De La Garza, Francisco

AU - Alarcón-Galván, Gabriela

AU - Zapata-Chavira, Homero

AU - De Jesús Sotelo-Gallegos, Ma

AU - Torres-Esquivel, Cipactli Nadjedja

AU - Sánchez-Fresno, Ethel

AU - Cantú-Sepúlveda, Daniel

AU - González-Saldivar, Gerardo

AU - Bernal-Ramirez, Judith

AU - Muñoz-Espinosa, Linda E.

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Background : Various hepatoprotective herbal products from plants are available in Mexico, where up to 85% of patients with liver disease use some form of complementary and alternative medicine. However, only few studies have reported on the biological evaluation of these products. Objective : Using a model of carbon tetrachloride (CCl4)-induced hepatotoxicity in rats, we evaluated the effects of commercial herbal extracts used most commonly in the metropolitan area of Monterrey, Mexico. Materials and Methods : The commercial products were identified through surveys in public areas. The effect of these products given with or without CCl4in rats was evaluated by measuring the serum concentrations of aspartate amino transferase (AST) and alanine amino transferase (ALT), and histopathological analysis. Legalon®was used as the standard drug. Results : The most commonly used herbal products were Hepatisan®capsules, Boldo capsules, Hepavida®capsules, Boldo infusion, and milk thistle herbal supplement (80% silymarin). None of the products tested was hepatotoxic according to transaminase and histological analyses. AST and ALT activities were significantly lower in the Hepavida+CCl4-treated group as compared with the CCl4-only group. AST and ALT activities in the silymarin, Hepatisan, and Boldo tea groups were similar to those in the CCl4group. The CCl4group displayed submassive confluent necrosis and mixed inflammatory infiltration. Both the Hepatisan+CCl4and Boldo tea+CCl4groups exhibited ballooning degeneration, inflammatory infiltration, and lytic necrosis. The silymarin+CCl4group exhibited microvesicular steatosis. The Hepavida+CCl4- and Legalon+CCL4-treated groups had lower percentages of necrotic cells as compared with the CCl4-treated group; this treatment was hepatoprotective against necrosis. Conclusion : Only Hepavida had a hepatoprotective effect.

AB - Background : Various hepatoprotective herbal products from plants are available in Mexico, where up to 85% of patients with liver disease use some form of complementary and alternative medicine. However, only few studies have reported on the biological evaluation of these products. Objective : Using a model of carbon tetrachloride (CCl4)-induced hepatotoxicity in rats, we evaluated the effects of commercial herbal extracts used most commonly in the metropolitan area of Monterrey, Mexico. Materials and Methods : The commercial products were identified through surveys in public areas. The effect of these products given with or without CCl4in rats was evaluated by measuring the serum concentrations of aspartate amino transferase (AST) and alanine amino transferase (ALT), and histopathological analysis. Legalon®was used as the standard drug. Results : The most commonly used herbal products were Hepatisan®capsules, Boldo capsules, Hepavida®capsules, Boldo infusion, and milk thistle herbal supplement (80% silymarin). None of the products tested was hepatotoxic according to transaminase and histological analyses. AST and ALT activities were significantly lower in the Hepavida+CCl4-treated group as compared with the CCl4-only group. AST and ALT activities in the silymarin, Hepatisan, and Boldo tea groups were similar to those in the CCl4group. The CCl4group displayed submassive confluent necrosis and mixed inflammatory infiltration. Both the Hepatisan+CCl4and Boldo tea+CCl4groups exhibited ballooning degeneration, inflammatory infiltration, and lytic necrosis. The silymarin+CCl4group exhibited microvesicular steatosis. The Hepavida+CCl4- and Legalon+CCL4-treated groups had lower percentages of necrotic cells as compared with the CCl4-treated group; this treatment was hepatoprotective against necrosis. Conclusion : Only Hepavida had a hepatoprotective effect.

U2 - 10.4103/0974-8490.112417

DO - 10.4103/0974-8490.112417

M3 - Article

SP - 150

EP - 156

JO - Pharmacognosy Research

JF - Pharmacognosy Research

SN - 0974-8490

ER -