Genetic Variability of Chikungunya Virus in Southern Mexico

Kame Galán-Huerta, Viviana Zomosa-Signoret, Román Vidaltamayo, Sandra Caballero-Sosa, Ildefonso Fernández-Salas, Javier Ramos-Jiménez, Ana María Rivas-Estilla

Research output: Contribution to journalArticle

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Abstract

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes Chikungunya
fever. CHIKV entered Mexico through the state of Chiapas in October 2014. To fully understand the Chikungunya fever outbreak that occurred in southern Chiapas during 2015, we evaluated 22 PCR-confirmed CHIKV-positive patients, identified CHIKV genetic variability, reconstructed viral dispersal, and assessed possible viral mutations. Viruses were isolated and E2, 6K, and E1 genes were sequenced. We applied phylogenetic and phylogeographic approaches, modeled mutations, and estimated selective pressure. Different CHIKV strains circulated in Chiapas during summer 2015. Three isolates grouped themselves in a well-supported clade. Estimates show that the outbreak started in Ciudad Hidalgo and posteriorly dispersed towards Tapachula and neighboring municipalities. We found six non-synonymous mutations in our isolates. Two mutations occurred in one isolate and the remaining mutations occurred in single isolates. Mutations E2 T116I and E2 K221R changed the protein surface in contact with the host cell receptors. We could not find positive selected sites in our CHIKV sequences from southern Chiapas. This is the first viral phylogeographic reconstruction in Mexico characterizing the CHIKV outbreak in southern Chiapas.
Original languageEnglish
Article number714
Number of pages16
JournalViruses
Volume11
Issue number8
DOIs
Publication statusPublished - 5 Aug 2019

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Chikungunya virus
Mexico
Mutation
Disease Outbreaks
Alphavirus
Culicidae
Membrane Proteins
Viruses
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

Cite this

Galán-Huerta, K., Zomosa-Signoret, V., Vidaltamayo, R., Caballero-Sosa, S., Fernández-Salas, I., Ramos-Jiménez, J., & Rivas-Estilla, A. M. (2019). Genetic Variability of Chikungunya Virus in Southern Mexico. Viruses, 11(8), [714]. https://doi.org/10.3390/v11080714
Galán-Huerta, Kame ; Zomosa-Signoret, Viviana ; Vidaltamayo, Román ; Caballero-Sosa, Sandra ; Fernández-Salas, Ildefonso ; Ramos-Jiménez, Javier ; Rivas-Estilla, Ana María. / Genetic Variability of Chikungunya Virus in Southern Mexico. In: Viruses. 2019 ; Vol. 11, No. 8.
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Galán-Huerta, K, Zomosa-Signoret, V, Vidaltamayo, R, Caballero-Sosa, S, Fernández-Salas, I, Ramos-Jiménez, J & Rivas-Estilla, AM 2019, 'Genetic Variability of Chikungunya Virus in Southern Mexico', Viruses, vol. 11, no. 8, 714. https://doi.org/10.3390/v11080714

Genetic Variability of Chikungunya Virus in Southern Mexico. / Galán-Huerta, Kame; Zomosa-Signoret, Viviana; Vidaltamayo, Román; Caballero-Sosa, Sandra; Fernández-Salas, Ildefonso ; Ramos-Jiménez, Javier; Rivas-Estilla, Ana María.

In: Viruses, Vol. 11, No. 8, 714, 05.08.2019.

Research output: Contribution to journalArticle

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Galán-Huerta K, Zomosa-Signoret V, Vidaltamayo R, Caballero-Sosa S, Fernández-Salas I, Ramos-Jiménez J et al. Genetic Variability of Chikungunya Virus in Southern Mexico. Viruses. 2019 Aug 5;11(8). 714. https://doi.org/10.3390/v11080714