Formation of immunoglobulin light chain amyloid oligomers in primary cutaneous nodular amyloidosis

A.L. Clos, C.A. Lasagna-Reeves, D.L. Castillo-Carranza, U. Sengupta, G.R. Jackson, B. Kelly, T.M. Beachkofsky, R. Kayed

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Background Primary cutaneous nodular amyloidosis (PCNA) is thought to be a plasma cell dyscrasia. The amyloid deposits are found in the dermis and subcutis, and they contain clonal immunoglobulin light chains, produced by a local proliferation of plasma cells. New insights into amyloid diseases have revealed that the pathology is due more to the presence of small, misfolded protein species termed oligomers than to the deposition of fibrillar material. Objectives To demonstrate the presence of amyloid oligomers in PCNA and to provide evidence that cutaneous amyloid diseases share a common pathogenic pathway similar to other amyloid diseases. Methods Immunohistochemical staining with conformation-specific and sequence-specific antibodies was used to localize different amyloid species of light chain immunoglobulins in a case of PCNA. Additionally, in vitro characterization of immunoglobulin oligomers and fibrils was performed to determine, through toxicity studies in a human keratinocyte cell line, which amyloidogenic form of the immunoglobulin is toxic in PCNA. Results Amyloid oligomers were identified in PCNA. Oligomers were mainly formed by lambda light chain immunoglobulins, and kappa light chain oligomers were detected in lesser amounts. Amyloid species were detected intra- and extracellularly. In addition, amyloid oligomers and fibrils, derived from unknown protein sources, were detected. This finding suggests that immunoglobulin amyloids can act as seeds capable of inducing the aggregation of heterogeneous proteins in the skin. Furthermore, cytotoxicity studies demonstrated that immunoglobulin oligomers, but not monomers or fibrils, are toxic to human keratinocytes. Conclusions These data indicate that PCNA has common pathways with other amyloid diseases with respect to protein misfolding and pathogenesis. Immunoglobulin oligomers may prove to be targets for the treatment of PCNA.

Original languageEnglish
Pages (from-to)1349-1354
Number of pages6
JournalBritish Journal of Dermatology
Volume165
Issue number6
DOIs
Publication statusPublished - Dec 2011
Externally publishedYes

Bibliographical note

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

  • Dermatology

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